Restriction map for
pFN21AB6957
(Circular) (Six-base) MAPSORT of: pFN21AB6957.seq Check: 3710 from: 1 to: 5344
pFN21AB6957 5344 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2462 2665 2462
Size: 203 5141
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4791 5059 1632
Size: 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 268
AfeI AGC'GCT
Cuts at: 2072 2072
Size: 5344
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 4243 5220 1203
Size: 3040 977 1327
Fragments arranged by size:
3040 1327 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 2404 1166
Size: 1238 4106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2231 1184
Size: 1047 4297
AlwNI CAG_nnn'CTG
Cuts at: 2250 3087 4659 2250
Size: 837 1572 2935
Fragments arranged by size:
2935 1572 837
ApaI G_GGCC'C
Cuts at: 2152 2159 2152
Size: 7 5337
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2653 2879 3031 5140 1493
Size: 1160 226 152 2109 1697
Fragments arranged by size:
2109 1697 1160 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2256 2552 2669 1870
Size: 73 313 296 117 4545
Fragments arranged by size:
4545 313 296 117 73
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2462 2665 4120 618
Size: 325 217 627 675 203 1455 1842
Fragments arranged by size:
1842 1455 675 627 325 217 203
BanII G_rGCy'C
Cuts at: 1391 2152 2159 2167 2382 2663 1391
Size: 761 7 8 215 281 4072
Fragments arranged by size:
4072 761 281 215 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2624 928
Size: 971 725 3648
Fragments arranged by size:
3648 971 725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3535 3569 1903
Size: 34 1598 34 3678
Fragments arranged by size:
3678 1598 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2205 2393
Size: 107 122 71 631 605 356 177 188
Cuts at: 2393 3955 136
Size: 1562 1525
Fragments arranged by size:
1562 1525 631 605 356 188 177 122
107 71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 2152 2159 3405 4561 1790
Size: 362 7 1246 1156 2573
Fragments arranged by size:
2573 1246 1156 362 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5344
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2190 2204 4004 1239
Size: 942 9 14 1800 2579
Fragments arranged by size:
2579 1800 942 14 9
Bpu10I CC'TnA_GC
Cuts at: 2540 2540
Size: 5344
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 3469 4334 4632 2054
Size: 1415 865 298 2766
Fragments arranged by size:
2766 1415 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4007 882
Size: 617 2508 2219
Fragments arranged by size:
2508 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3135 493
Size: 711 1931 2702
Fragments arranged by size:
2702 1931 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2549 3151 3533 4168
Size: 53 83 186 936 1016 602 382 635
Cuts at: 4168 275
Size: 1451
Fragments arranged by size:
1451 1016 936 635 602 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3777 4175 4449 4596 5007 3777
Size: 398 274 147 411 4114
Fragments arranged by size:
4114 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2209 2390 1186
Size: 1023 181 4140
Fragments arranged by size:
4140 1023 181
BseYI C'CCAG_C
Cuts at: 2089 2310 4547 5043 2089
Size: 221 2237 496 2390
Fragments arranged by size:
2390 2237 496 221
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4771
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2794 3555 4583 1540
Size: 275 186 793 761 1028 2301
Fragments arranged by size:
2301 1028 793 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2382 2609 3405 3490 4561 2382
Size: 227 796 85 1071 3165
Fragments arranged by size:
3165 1071 796 227 85
BsmI GAATG_Cn'
Cuts at: 2855 2948 2855
Size: 93 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2152 2159 2167 2382 2609 2663 3405
Size: 399 362 7 8 215 227 54 742
Cuts at: 3405 3490 4561 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 362 227 215 85
54 8 7
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 2412 3233 4963 2412
Size: 821 1730 2793
Fragments arranged by size:
2793 1730 821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2199 2699 844
Size: 1355 500 3489
Fragments arranged by size:
3489 1355 500
BsrBI CCG'CTC
Cuts at: 1394 1823 3231 1394
Size: 429 1408 3507
Fragments arranged by size:
3507 1408 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2181 2199 3988 4175 1700
Size: 110 371 18 1789 187 2869
Fragments arranged by size:
2869 1789 371 187 110 18
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5344
BssSI C'ACGA_G
Cuts at: 3404 4416 3404
Size: 1012 4332
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5344
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 2104 2443 1184
Size: 920 339 4085
Fragments arranged by size:
4085 920 339
BstYI r'GATC_y
Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339
Size: 702 367 385 17 1452 86 12 346
Cuts at: 5339 1972
Size: 1977
Fragments arranged by size:
1977 1452 702 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2063 2192 2063
Size: 129 5215
BtgI C'CryG_G
Cuts at: 513 1065 2100 2110 2610 513
Size: 552 1035 10 500 3247
Fragments arranged by size:
3247 1035 552 500 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2121 487
Size: 683 56 895 3710
Fragments arranged by size:
3710 895 683 56
BtsI GCAGTG_nn'
Cuts at: 2564 2860 3654 3674 2564
Size: 296 794 20 4234
Fragments arranged by size:
4234 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2648 2648
Size: 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2526 2791
Size: 54 1425 50 275 18 267 429 265
Cuts at: 2791 3680 5309 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 429 275 267 265 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2791 1537
Size: 275 979 4090
Fragments arranged by size:
4090 979 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 1353
Size: 1921 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3931 4305 4451 387
Size: 1064 138 2342 374 146 1280
Fragments arranged by size:
2342 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2380 2380
Size: 5344
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2190 2204 3407 4004 4791 5059
Size: 393 549 9 14 1203 597 787 268
Cuts at: 5059 1239
Size: 1524
Fragments arranged by size:
1524 1203 787 597 549 393 268 14
9
EcoO109I rG'GnC_Cy
Cuts at: 2029 2038 2054 2148 2149 2156 2307 2565 2029
Size: 9 16 94 1 7 151 258 4808
Fragments arranged by size:
4808 258 151 94 16 9 7 1
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 2074 4491 1678
Size: 396 2417 2531
Fragments arranged by size:
2531 2417 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KpnI G_GTAC'C
Cuts at: 2466 2669 2466
Size: 203 5141
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4458 4642 1305
Size: 3153 184 2007
Fragments arranged by size:
3153 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2404 3302 3661 3820 518
Size: 648 1238 898 359 159 2042
Fragments arranged by size:
2042 1238 898 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2132 2304 2583 2760 3437 4585 4830 1606
Size: 526 172 279 177 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 526 279 245 177 172
NcoI C'CATG_G
Cuts at: 513 1065 2110 513
Size: 552 1045 3747
Fragments arranged by size:
3747 1045 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2294 2702 4247 1132
Size: 97 1065 408 1545 2229
Fragments arranged by size:
2229 1545 1065 408 97
PasI CC'CwG_GG
Cuts at: 1348 2152 2476 1348
Size: 804 324 4216
Fragments arranged by size:
4216 804 324
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2250 2482 1108
Size: 1142 232 3970
Fragments arranged by size:
3970 1142 232
PfoI T'CCnGG_A
Cuts at: 2231 2428 2231
Size: 197 5147
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2307 2565 2307
Size: 258 5086
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PspOMI G'GGCC_C
Cuts at: 2148 2155 2148
Size: 7 5337
PstI C_TGCA'G
Cuts at: 830 2635 2696 830
Size: 1805 61 3478
Fragments arranged by size:
3478 1805 61
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
PvuII CAG'CTG
Cuts at: 2132 2583 2132
Size: 451 4893
SacI G_AGCT'C
Cuts at: 2382 2382
Size: 5344
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SanDI GG'GwC_CC
Cuts at: 2307 2307
Size: 5344
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2578 2631 2692 3827 4508 4699 826
Size: 219 1533 53 61 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191 61 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2028 2028
Size: 5344
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2258 2671 1872
Size: 386 413 4545
Fragments arranged by size:
4545 413 386
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3448 4349 4611 820
Size: 197 926 90 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 197 90
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2110 2334 2783 513
Size: 552 1045 224 449 3074
Fragments arranged by size:
3074 1045 552 449 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2489 3371 3388 3541 3726 4171
Size: 1255 758 151 882 17 153 185 445
Cuts at: 4171 325
Size: 1498
Fragments arranged by size:
1498 1255 882 758 445 185 153 151
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3590 5302 96
Size: 274 80 33 51 1443 1613 1712 138
Fragments arranged by size:
1712 1613 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2256 2669 1870
Size: 386 413 4545
Fragments arranged by size:
4545 413 386
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SapI
SexAI SfoI SrfI SwaI XcmI