Restriction map for
pFN21AB6957
(Linear) (Six-base) MAPSORT of: KIBB6957.seq Check: 3760 from: 1 to: 639
KIBB6957.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
Acc65I G'GTAC_C
Cuts at: 0 457 639
Size: 457 182
AfeI AGC'GCT
Cuts at: 0 67 639
Size: 67 572
AleI CACnn'nnGTG
Cuts at: 0 399 639
Size: 399 240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 226 639
Size: 226 413
AlwNI CAG_nnn'CTG
Cuts at: 0 245 639
Size: 245 394
ApaI G_GGCC'C
Cuts at: 0 147 154 639
Size: 147 7 485
Fragments arranged by size:
485 147 7
AvaI C'yCGr_G
Cuts at: 0 251 547 639
Size: 251 296 92
Fragments arranged by size:
296 251 92
BanI G'GyrC_C
Cuts at: 0 457 639
Size: 457 182
BanII G_rGCy'C
Cuts at: 0 147 154 162 377 639
Size: 147 7 8 215 262
Fragments arranged by size:
262 215 147 8 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 619 639
Size: 619 20
BglI GCCn_nnn'nGGC
Cuts at: 0 23 200 388 639
Size: 23 177 188 251
Fragments arranged by size:
251 188 177 23
Bme1580I G_kGCm'C
Cuts at: 0 147 154 639
Size: 147 7 485
Fragments arranged by size:
485 147 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 185 199 639
Size: 176 9 14 440
Fragments arranged by size:
440 176 14 9
Bpu10I CC'TnA_GC
Cuts at: 0 535 639
Size: 535 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 639
Size: 49 590
BsaHI Gr'CG_yC
Cuts at: 0 544 639
Size: 544 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 385 639
Size: 204 181 254
Fragments arranged by size:
254 204 181
BseYI C'CCAG_C
Cuts at: 0 84 305 639
Size: 84 221 334
Fragments arranged by size:
334 221 84
BsiHKAI G_wGCw'C
Cuts at: 0 377 604 639
Size: 377 227 35
Fragments arranged by size:
377 227 35
Bsp1286I G_dGCh'C
Cuts at: 0 147 154 162 377 604 639
Size: 147 7 8 215 227 35
Fragments arranged by size:
227 215 147 35 8 7
BspHI T'CATG_A
Cuts at: 0 407 639
Size: 407 232
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 639
Size: 194 445
BsrFI r'CCGG_y
Cuts at: 0 176 194 639
Size: 176 18 445
Fragments arranged by size:
445 176 18
BstEII G'GTnAC_C
Cuts at: 0 99 438 639
Size: 99 339 201
Fragments arranged by size:
339 201 99
Bsu36I CC'TnA_GG
Cuts at: 0 58 187 639
Size: 58 129 452
Fragments arranged by size:
452 129 58
BtgI C'CryG_G
Cuts at: 0 95 105 605 639
Size: 95 10 500 34
Fragments arranged by size:
500 95 34 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 639
Size: 116 523
BtsI GCAGTG_nn'
Cuts at: 0 559 639
Size: 559 80
EaeI y'GGCC_r
Cuts at: 0 92 521 639
Size: 92 429 118
Fragments arranged by size:
429 118 92
EcoICRI GAG'CTC
Cuts at: 0 375 639
Size: 375 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 185 199 639
Size: 176 9 14 440
Fragments arranged by size:
440 176 14 9
EcoO109I rG'GnC_Cy
Cuts at: 0 24 33 49 143 144 151 302 560
Size: 24 9 16 94 1 7 151 258
Cuts at: 560 639
Size: 79
Fragments arranged by size:
258 151 94 79 24 16 9 7
1
HaeII r_GCGC'y
Cuts at: 0 69 639
Size: 69 570
KpnI G_GTAC'C
Cuts at: 0 461 639
Size: 461 178
MslI CAynn'nnrTG
Cuts at: 0 399 639
Size: 399 240
MspA1I CmG'CkG
Cuts at: 0 127 299 578 639
Size: 127 172 279 61
Fragments arranged by size:
279 172 127 61
NcoI C'CATG_G
Cuts at: 0 105 639
Size: 105 534
NspI r_CATG'y
Cuts at: 0 289 639
Size: 289 350
PasI CC'CwG_GG
Cuts at: 0 147 471 639
Size: 147 324 168
Fragments arranged by size:
324 168 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 245 477 639
Size: 245 232 162
Fragments arranged by size:
245 232 162
PfoI T'CCnGG_A
Cuts at: 0 226 423 639
Size: 226 197 216
Fragments arranged by size:
226 216 197
PpuMI rG'GwC_Cy
Cuts at: 0 302 560 639
Size: 302 258 79
Fragments arranged by size:
302 258 79
PspOMI G'GGCC_C
Cuts at: 0 143 150 639
Size: 143 7 489
Fragments arranged by size:
489 143 7
PstI C_TGCA'G
Cuts at: 0 630 639
Size: 630 9
PvuII CAG'CTG
Cuts at: 0 127 578 639
Size: 127 451 61
Fragments arranged by size:
451 127 61
SacI G_AGCT'C
Cuts at: 0 377 639
Size: 377 262
SanDI GG'GwC_CC
Cuts at: 0 302 639
Size: 302 337
SfcI C'TryA_G
Cuts at: 0 573 626 639
Size: 573 53 13
Fragments arranged by size:
573 53 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 23 639
Size: 23 616
SmaI CCC'GGG
Cuts at: 0 253 639
Size: 253 386
SmlI C'TyrA_G
Cuts at: 0 28 639
Size: 28 611
StyI C'CwwG_G
Cuts at: 0 105 329 639
Size: 105 224 310
Fragments arranged by size:
310 224 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 333 484 639
Size: 333 151 155
Fragments arranged by size:
333 155 151
XmaI C'CCGG_G
Cuts at: 0 251 639
Size: 251 388
Enzymes that do cut:
Acc65I AfeI AleI AlfI AlwNI ApaI AvaI BanI
BanII BbsI BglI Bme1580I BpmI Bpu10I BpuEI BsaHI
BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrFI BstEII
Bsu36I BtgI BtgZI BtsI EaeI EcoICRI Eco57MI EcoO109I
HaeII KpnI MslI MspA1I NcoI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI
SfiI SmaI SmlI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI