################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AcyI AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BglI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KpnI KspI MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflII 49 CTTAAG C^TTAAG AhdI 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1316 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 57 TGGCCA TGG^CCA BanII 1186 GAGCTC G[GA]GC[CT]^C BciVI 1023 GGATAC GTATCC(6/5) BfrI 49 CTTAAG C^TTAAG BglII 1897 AGATCT A^GATCT BsaAI 1582 TACGTG [CT]AC^GT[GA] BsaI 44 GAGACC GGTCTC(1/5) BsmFI 1865 GGGAC GGGAC(10/14) Bsp1407I 754 TGTACA T^GTACA BspHI 1934 TCATGA T^CATGA BspLU11I 406 ACATGT A^CATGT BspMI 1947 ACCTGC ACCTGC(4/8) BsrFI 1833 GCCGGT [GA]^CCGG[CT] BsrGI 754 TGTACA T^GTACA Bst1107I 1943 GTATAC GTA^TAC BstBI 1910 TTCGAA TT^CGAA BstZ17I 1943 GTATAC GTA^TAC Cfr10I 1833 GCCGGT [GA]^CCGG[CT] DsaI 955 CCACGG C^C[GA][CT]GG EaeI 57 TGGCCA [CT]^GGCC[GA] Eam1105I 987 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 426 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 872 GAATTC G^AATTC HapII 1834 CCGG C^CGG HincII 1669 GTTAAC GT[CT]^[GA]AC HindII 1669 GTTAAC GT[CT]^[GA]AC HindIII 865 AAGCTT A^AGCTT HpaI 1669 GTTAAC GTT^AAC HpaII 1834 CCGG C^CGG MluNI 57 TGGCCA TGG^CCA MscI 57 TGGCCA TGG^CCA MspI 1834 CCGG C^CGG NspV 1910 TTCGAA TT^CGAA PflMI 1315 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1934 TCATGA T^CATGA SacI 1186 GAGCTC GAGCT^C ScaI 1756 AGTACT AGT^ACT SfuI 1910 TTCGAA TT^CGAA SpeI 1924 ACTAGT A^CTAGT SspBI 754 TGTACA T^GTACA Van91I 1315 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 567 CCACCCTGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AclI 975 AACGTT AA^CGTT 1666 AACGTT AflIII 406 ACATGT A^C[GA][CT]GT 1583 ACGTGT Alw44I 352 GTGCAC G^TGCAC 802 GTGCAC ApaLI 352 GTGCAC G^TGCAC 802 GTGCAC Asp700I 609 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1521 GAATGCCTTC BfaI 1640 CTAG C^TAG 1925 CTAG BsmAI 17 GAGAC GTCTC(1/5) 44 GAGAC BsrDI 795 CATTGC GCAATG(2/0) 1961 GCAATG BstYI 1897 AGATCT [GA]^GATC[CT] 1967 AGATCC EcoT14I 167 CCTTGG C^C[AT][AT]GG 1095 CCAAGG MaeI 1640 CTAG C^TAG 1925 CTAG MflI 1897 AGATCT [GA]^GATC[CT] 1967 AGATCC MwoI 247 GCTGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 523 GCAGGCATAGC NspI 406 ACATGT [GA]CATG^[CT] 1655 GCATGT Psp1406I 975 AACGTT AA^CGTT 1666 AACGTT SmlI 49 CTTAAG C^T[CT][GA]AG 670 CTTGAG SspI 276 AATATT AAT^ATT 1390 AATATT StyI 167 CCTTGG C^C[AT][AT]GG 1095 CCAAGG XhoII 1897 AGATCT [GA]^GATC[CT] 1967 AGATCC XmnI 609 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1521 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I