(Linear) (Six-base) MAPSORT of: KIEE6724.seq Check: 1683 from: 1 to: 2079 KIEE6724.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 470 740 1944 2079 Size: 470 270 1204 135 Fragments arranged by size: 1204 470 270 135 AclI AA'CG_TT Cuts at: 0 976 1667 2079 Size: 976 691 412 Fragments arranged by size: 976 691 412 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 269 412 593 628 1019 1370 2079 Size: 269 143 181 35 391 351 709 Fragments arranged by size: 709 391 351 269 181 143 35 AflII C'TTAA_G Cuts at: 0 49 2079 Size: 49 2030 AflIII A'CryG_T Cuts at: 0 406 1583 2079 Size: 406 1177 496 Fragments arranged by size: 1177 496 406 AhdI GACnn_n'nnGTC Cuts at: 0 992 2079 Size: 992 1087 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 188 220 2079 Size: 188 32 1859 Fragments arranged by size: 1859 188 32 AlwNI CAG_nnn'CTG Cuts at: 0 1321 2079 Size: 1321 758 ApaLI G'TGCA_C Cuts at: 0 352 802 2079 Size: 352 450 1277 Fragments arranged by size: 1277 450 352 ApoI r'AATT_y Cuts at: 0 158 305 612 872 1259 2079 Size: 158 147 307 260 387 820 Fragments arranged by size: 820 387 307 260 158 147 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1016 1049 2079 Size: 1016 33 1030 Fragments arranged by size: 1030 1016 33 BanII G_rGCy'C Cuts at: 0 1190 2079 Size: 1190 889 BciVI GTATCCnnnnn_n' Cuts at: 0 1017 2079 Size: 1017 1062 BglII A'GATC_T Cuts at: 0 1897 2079 Size: 1897 182 Bme1580I G_kGCm'C Cuts at: 0 356 806 2079 Size: 356 450 1273 Fragments arranged by size: 1273 450 356 BmgBI CAC'GTC Cuts at: 0 707 2079 Size: 707 1372 BmrI ACTGGGnnnn_n' Cuts at: 0 1626 2079 Size: 1626 453 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 59 245 1581 2079 Size: 49 10 186 1336 498 Fragments arranged by size: 1336 498 186 49 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 691 2079 Size: 691 1388 BsaI GGTCTCn'nnnn_ Cuts at: 0 38 2079 Size: 38 2041 BsaAI yAC'GTr Cuts at: 0 1584 2079 Size: 1584 495 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 4 34 68 98 335 365 452 482 Size: 4 30 34 30 237 30 87 30 Cuts at: 482 1792 1822 2079 Size: 1310 30 257 Fragments arranged by size: 1310 257 237 87 34 30 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 56 1093 1222 2079 Size: 56 1037 129 857 Fragments arranged by size: 1037 857 129 56 BseYI C'CCAG_C Cuts at: 0 1813 2079 Size: 1813 266 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 676 1449 1576 2079 Size: 169 507 773 127 503 Fragments arranged by size: 773 507 503 169 127 BsiHKAI G_wGCw'C Cuts at: 0 105 356 561 806 1190 1754 2079 Size: 105 251 205 245 384 564 325 Fragments arranged by size: 564 384 325 251 245 205 105 BsmI GAATG_Cn' Cuts at: 0 39 1512 1527 2079 Size: 39 1473 15 552 Fragments arranged by size: 1473 552 39 15 Bsp1286I G_dGCh'C Cuts at: 0 105 356 561 806 1190 1754 2079 Size: 105 251 205 245 384 564 325 Fragments arranged by size: 564 384 325 251 245 205 105 BspHI T'CATG_A Cuts at: 0 1934 2079 Size: 1934 145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1956 2079 Size: 1956 123 BsrDI GCAATG_nn' Cuts at: 0 794 1968 2079 Size: 794 1174 111 Fragments arranged by size: 1174 794 111 BsrFI r'CCGG_y Cuts at: 0 1833 2079 Size: 1833 246 BsrGI T'GTAC_A Cuts at: 0 754 2079 Size: 754 1325 BstBI TT'CG_AA Cuts at: 0 1911 2079 Size: 1911 168 BstYI r'GATC_y Cuts at: 0 1897 1967 2079 Size: 1897 70 112 Fragments arranged by size: 1897 112 70 BstZ17I GTA'TAC Cuts at: 0 1945 2079 Size: 1945 134 BtgI C'CryG_G Cuts at: 0 955 2079 Size: 955 1124 BtsI GCAGTG_nn' Cuts at: 0 645 2079 Size: 645 1434 EaeI y'GGCC_r Cuts at: 0 57 2079 Size: 57 2022 EarI CTCTTCn'nnn_ Cuts at: 0 123 845 995 1283 1469 2079 Size: 123 722 150 288 186 610 Fragments arranged by size: 722 610 288 186 150 123 EcoICRI GAG'CTC Cuts at: 0 1188 2079 Size: 1188 891 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 59 245 269 412 593 628 1019 Size: 49 10 186 24 143 181 35 391 Cuts at: 1019 1370 1581 2079 Size: 351 211 498 Fragments arranged by size: 498 391 351 211 186 181 143 49 35 24 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 430 2079 Size: 430 1649 EcoO109I rG'GnC_Cy Cuts at: 0 229 423 855 1318 1866 2079 Size: 229 194 432 463 548 213 Fragments arranged by size: 548 463 432 229 213 194 EcoRI G'AATT_C Cuts at: 0 872 2079 Size: 872 1207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 598 2079 Size: 598 1481 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 68 100 978 1010 1606 1638 2032 2064 Size: 68 32 878 32 596 32 394 32 Cuts at: 2064 2079 Size: 15 Fragments arranged by size: 878 596 394 68 32 32 32 32 15 HincII GTy'rAC Cuts at: 0 1671 2079 Size: 1671 408 HindIII A'AGCT_T Cuts at: 0 865 2079 Size: 865 1214 HpaI GTT'AAC Cuts at: 0 1671 2079 Size: 1671 408 MscI TGG'CCA Cuts at: 0 59 2079 Size: 59 2020 MslI CAynn'nnrTG Cuts at: 0 68 394 799 1175 1247 2079 Size: 68 326 405 376 72 832 Fragments arranged by size: 832 405 376 326 72 68 NspI r_CATG'y Cuts at: 0 410 1659 2079 Size: 410 1249 420 Fragments arranged by size: 1249 420 410 PciI A'CATG_T Cuts at: 0 406 2079 Size: 406 1673 PflMI CCAn_nnn'nTGG Cuts at: 0 1321 2079 Size: 1321 758 PpuMI rG'GwC_Cy Cuts at: 0 229 1318 1866 2079 Size: 229 1089 548 213 Fragments arranged by size: 1089 548 229 213 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1905 1937 2079 Size: 1905 32 142 Fragments arranged by size: 1905 142 32 SacI G_AGCT'C Cuts at: 0 1190 2079 Size: 1190 889 SanDI GG'GwC_CC Cuts at: 0 1866 2079 Size: 1866 213 ScaI AGT'ACT Cuts at: 0 1758 2079 Size: 1758 321 SfcI C'TryA_G Cuts at: 0 1547 1575 1715 1973 2079 Size: 1547 28 140 258 106 Fragments arranged by size: 1547 258 140 106 28 SmlI C'TyrA_G Cuts at: 0 49 670 2079 Size: 49 621 1409 Fragments arranged by size: 1409 621 49 SpeI A'CTAG_T Cuts at: 0 1924 2079 Size: 1924 155 SspI AAT'ATT Cuts at: 0 278 1392 2079 Size: 278 1114 687 Fragments arranged by size: 1114 687 278 StyI C'CwwG_G Cuts at: 0 167 1095 2079 Size: 167 928 984 Fragments arranged by size: 984 928 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 140 2079 Size: 140 1939 TatI w'GTAC_w Cuts at: 0 754 1756 2079 Size: 754 1002 323 Fragments arranged by size: 1002 754 323 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 335 367 2079 Size: 335 32 1712 Fragments arranged by size: 1712 335 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 574 2079 Size: 574 1505 XmnI GAAnn'nnTTC Cuts at: 0 613 1525 2079 Size: 613 912 554 Fragments arranged by size: 912 613 554 Enzymes that do cut: AccI AclI AcuI AflII AflIII AhdI AloI AlwNI ApaLI ApoI BaeI BanII BciVI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstBI BstYI BstZ17I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII HpaI MscI MslI NspI PciI PflMI PpuMI PsrI SacI SanDI ScaI SfcI SmlI SpeI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AgeI AleI AlfI ApaI AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI BstAPI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI