################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI Asp700I Asp718I AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI DsaI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HindIII KasI KpnI KspI MaeII MamI MluI MroI NarI NcoI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AccI 574 GTCGAC GT^[AC][GT]AC AcsI 286 AAATTC [GA]^AATT[CT] AcyI 184 GGCGTC G[GA]^CG[CT]C AhdI 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 596 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 880 GGGCCC GGGCC^C ApoI 286 AAATTC [GA]^AATT[CT] AseI 415 ATTAAT AT^TAAT AsnI 415 ATTAAT AT^TAAT AspEI 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1080 GAGCAC G[AT]GC[AT]^C AspI 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 6 CCCGGG C^[CT]CG[GA]G AvaII 709 GGTCC G^G[AT]CC BalI 198 TGGCCA TGG^CCA BcgI 1160 GCAACAATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 236 AGATCT A^GATCT BlpI 99 GCTGAGC GC^T[ACGT]AGC Bpu1102I 99 GCTGAGC GC^T[ACGT]AGC BsaHI 184 GGCGTC G[GA]^CG[CT]C BsiEI 41 CGGCCG CG[GA][CT]^CG BsiHKAI 1080 GAGCAC G[AT]GC[AT]^C BsoBI 6 CCCGGG C^[CT]CG[GA]G BspDI 949 ATCGAT AT^CGAT BspHI 1298 TCATGA T^CATGA BspMI 744 ACCTGC ACCTGC(4/8) BsrFI 46 GCCGGC [GA]^CCGG[CT] BssSI 1039 CACGAG CACGAG(-5/-1) BstYI 236 AGATCT [GA]^GATC[CT] CelII 99 GCTGAGC GC^T[ACGT]AGC Cfr10I 46 GCCGGC [GA]^CCGG[CT] ClaI 949 ATCGAT AT^CGAT DraII 879 GGGGCCC [GA]G^G[ACGT]CC[CT] EagI 41 CGGCCG C^GGCCG Eam1105I 663 GACATATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 232 GAAGAG CTCTTC(1/4) EclXI 41 CGGCCG C^GGCCG Eco52I 41 CGGCCG C^GGCCG EcoNI 1020 CCTATGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 879 GGGGCCC [GA]G^G[ACGT]CC[CT] FauI 176 CCCGC CCCGC(4/6) HgaI 185 GCGTC GACGC(5/10) Hin1I 184 GGCGTC G[GA]^CG[CT]C HpaI 1320 GTTAAC GTT^AAC Ksp632I 232 GAAGAG CTCTTC(1/4) MfeI 319 CAATTG C^AATTG MflI 236 AGATCT [GA]^GATC[CT] MluNI 198 TGGCCA TGG^CCA MscI 198 TGGCCA TGG^CCA MspA1I 202 CAGCTG C[AC]G^C[GT]G MunI 319 CAATTG C^AATTG NaeI 46 GCCGGC GCC^GGC NdeI 665 CATATG CA^TATG NgoMIV 46 GCCGGC G^CCGGC NotI 40 GCGGCCGC GC^GGCCGC PflFI 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 415 ATTAAT AT^TAAT PvuII 202 CAGCTG CAG^CTG RcaI 1298 TCATGA T^CATGA SalI 574 GTCGAC G^TCGAC ScaI 1003 AGTACT AGT^ACT SfcI 835 CTACAG C^T[GA][CT]AG SmaI 6 CCCGGG CCC^GGG SmlI 713 CTCAAG C^T[CT][GA]AG SspI 1177 AATATT AAT^ATT Tth111I 365 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 236 AGATCT [GA]^GATC[CT] XmaCI 6 CCCGGG C^CCGGG XmaI 6 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 866 GTAC GT^AC 1004 GTAC BanII 25 GGGCTC G[GA]GC[CT]^C 880 GGGCCC BbvI 138 GCAGC GCAGC(8/12) 204 GCTGC BfaI 79 CTAG C^TAG 1138 CTAG BsgI 205 CTGCAC GTGCAG(16/14) 975 CTGCAC BsmAI 1049 GAGAC GTCTC(1/5) 984 GTCTC BsmI 641 GAATGC GAATGC(1/-1) 1308 GAATGC BstAPI 739 GCATTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 772 GCAAATCCTGC HincII 574 GTCGAC GT[CT]^[GA]AC 1320 GTTAAC HindII 574 GTCGAC GT[CT]^[GA]AC 1320 GTTAAC HphI 330 TCACC GGTGA(8/7) 1289 TCACC MaeI 79 CTAG C^TAG 1138 CTAG MslI 331 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1149 CATTTTGGTG NheI 78 GCTAGC G^CTAGC 1137 GCTAGC RsaI 866 GTAC GT^AC 1004 GTAC SfaNI 931 GATGC GCATC(5/9) 424 GCATC TseI 138 GCAGC G^C[AT]GC 204 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AluI AlwI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I