(Circular) (Six-base) MAPSORT of: pFN21AE6428.seq Check: 7839 from: 1 to: 6085 pFN21AE6428 6085 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3895 278 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 3428 1937 Size: 643 848 4594 Fragments arranged by size: 4594 848 643 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2074 2256 2981 4148 5532 5800 1632 Size: 442 182 725 1167 1384 268 1917 Fragments arranged by size: 1917 1384 1167 725 442 268 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 2673 4814 2673 Size: 2141 3944 AleI CACnn'nnGTG Cuts at: 1166 2340 1166 Size: 1174 4911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3145 1184 Size: 1961 4124 AlwNI CAG_nnn'CTG Cuts at: 2606 3828 5400 2606 Size: 1222 1572 3291 Fragments arranged by size: 3291 1572 1222 ApaI G_GGCC'C Cuts at: 2889 2889 Size: 6085 ApaLI G'TGCA_C Cuts at: 4142 5298 4142 Size: 1156 4929 ApoI r'AATT_y Cuts at: 1493 2291 3394 3620 3772 5881 1493 Size: 798 1103 226 152 2109 1697 Fragments arranged by size: 2109 1697 1103 798 226 152 AseI AT'TA_AT Cuts at: 160 1032 2421 4639 160 Size: 872 1389 2218 1606 Fragments arranged by size: 2218 1606 1389 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2011 3410 1870 Size: 73 68 1399 4545 Fragments arranged by size: 4545 1399 73 68 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 4861 618 Size: 325 217 627 1619 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2889 3404 1391 Size: 643 855 515 4072 Fragments arranged by size: 4072 855 643 515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3154 3188 4276 4310 1903 Size: 34 1217 34 1088 34 3678 Fragments arranged by size: 3678 1217 1088 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4696 136 Size: 107 122 71 631 605 3024 1525 Fragments arranged by size: 3024 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2241 6080 2241 Size: 3839 2246 BlpI GC'TnA_GC Cuts at: 2105 3502 2105 Size: 1397 4688 Bme1580I G_kGCm'C Cuts at: 1790 2889 4146 5302 1790 Size: 1099 1257 1156 2573 Fragments arranged by size: 2573 1257 1156 1099 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3816 4774 442 Size: 416 53 83 399 24 2399 958 1753 Fragments arranged by size: 2399 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2087 3146 1056 Size: 1031 1059 3995 Fragments arranged by size: 3995 1059 1031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2180 3222 4745 1239 Size: 893 48 1042 1523 2579 Fragments arranged by size: 2579 1523 1042 893 48 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2703 4210 5075 5373 2703 Size: 1507 865 298 3415 Fragments arranged by size: 3415 1507 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2190 3892 4274 4909 Size: 53 83 186 936 657 1702 382 635 Cuts at: 4909 275 Size: 1451 Fragments arranged by size: 1702 1451 936 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4518 4916 5190 5337 5748 4518 Size: 398 274 147 411 4855 Fragments arranged by size: 4855 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3045 3075 1178 Size: 30 165 30 1642 30 4188 Fragments arranged by size: 4188 1642 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2076 2426 3047 1186 Size: 890 350 621 4224 Fragments arranged by size: 4224 890 621 350 BseYI C'CCAG_C Cuts at: 5288 5784 5288 Size: 496 5589 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2965 1163 Size: 573 459 770 4283 Fragments arranged by size: 4283 770 573 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3535 4296 5324 1540 Size: 275 186 48 1486 761 1028 2301 Fragments arranged by size: 2301 1486 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 3089 4146 4231 5302 3089 Size: 1057 85 1071 3872 Fragments arranged by size: 3872 1071 1057 85 BsmI GAATG_Cn' Cuts at: 2652 3319 3596 3689 2652 Size: 667 277 93 5048 Fragments arranged by size: 5048 667 277 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2889 3089 3404 4146 4231 5302 Size: 399 244 855 200 315 742 85 1071 Cuts at: 5302 1391 Size: 2174 Fragments arranged by size: 2174 1071 855 742 399 315 244 200 85 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3303 3974 5704 3303 Size: 671 1730 3684 Fragments arranged by size: 3684 1730 671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2758 3440 844 Size: 1914 682 3489 Fragments arranged by size: 3489 1914 682 BsrBI CCG'CTC Cuts at: 1394 1823 3972 1394 Size: 429 2149 3507 Fragments arranged by size: 3507 2149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 4729 4916 1700 Size: 110 241 2678 187 2869 Fragments arranged by size: 2869 2678 241 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 3044 4145 5157 3044 Size: 1101 1012 3972 Fragments arranged by size: 3972 1101 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2750 2783 794 Size: 1956 33 4096 Fragments arranged by size: 4096 1956 33 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 2241 3415 3782 4167 4184 5636 5722 5734 Size: 269 1174 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1174 385 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2949 487 Size: 683 56 1723 3623 Fragments arranged by size: 3623 1723 683 56 BtsI GCAGTG_nn' Cuts at: 3601 4395 4415 3601 Size: 794 20 5271 Fragments arranged by size: 5271 794 20 ClaI AT'CG_AT Cuts at: 1554 2955 1554 Size: 1401 4684 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2046 2203 Size: 54 1425 50 275 18 195 21 157 Cuts at: 2203 3532 4421 6050 8 Size: 1329 889 1629 43 Fragments arranged by size: 1629 1425 1329 889 275 195 157 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3532 1537 Size: 275 234 1486 4090 Fragments arranged by size: 4090 1486 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 4015 1353 Size: 879 1783 3423 Fragments arranged by size: 3423 1783 879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2084 4672 5046 5192 387 Size: 1064 138 495 2588 374 146 1280 Fragments arranged by size: 2588 1280 1064 495 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2132 2180 2256 2981 3222 4148 Size: 393 442 58 48 76 725 241 926 Cuts at: 4148 4745 5532 5800 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 926 787 725 597 442 393 268 241 76 58 48 EcoNI CCTnn'n_nnAGG Cuts at: 3029 3029 Size: 6085 EcoO109I rG'GnC_Cy Cuts at: 2885 2885 Size: 6085 EcoRI G'AATT_C Cuts at: 3394 3772 3394 Size: 378 5707 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2437 2439 2469 2659 Size: 32 135 32 835 30 2 30 190 Cuts at: 2659 2691 3073 3105 4726 4758 4800 4832 1373 Size: 32 382 32 1621 32 42 32 2626 Fragments arranged by size: 2626 1621 835 382 190 135 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3327 3429 3675 669 Size: 988 281 643 746 102 246 3079 Fragments arranged by size: 3079 988 746 643 281 246 102 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3327 3675 3327 Size: 348 5737 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 2324 3684 2324 Size: 1360 4725 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2566 5199 5383 1305 Size: 1261 2633 184 2007 Fragments arranged by size: 2633 2007 1261 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2205 10 Size: 54 1425 343 373 3890 Fragments arranged by size: 3890 1425 373 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2340 3158 4043 4402 4561 518 Size: 648 1174 818 885 359 159 2042 Fragments arranged by size: 2042 1174 885 818 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2209 3501 4178 5326 5571 1606 Size: 603 1292 677 1148 245 2120 Fragments arranged by size: 2120 1292 1148 677 603 245 NaeI GCC'GGC Cuts at: 2053 2053 Size: 6085 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 2671 387 Size: 2284 3801 NgoMIV G'CCGG_C Cuts at: 2051 2051 Size: 6085 NheI G'CTAG_C Cuts at: 1052 2083 3142 1052 Size: 1031 1059 3995 Fragments arranged by size: 3995 1059 1031 NotI GC'GGCC_GC Cuts at: 2046 3532 2046 Size: 1486 4599 NspI r_CATG'y Cuts at: 1132 1229 2772 3126 3211 3443 4988 1132 Size: 97 1543 354 85 232 1545 2229 Fragments arranged by size: 2229 1545 1543 354 232 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PfoI T'CCnGG_A Cuts at: 2193 2193 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6085 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2885 2885 Size: 6085 PstI C_TGCA'G Cuts at: 830 3437 830 Size: 2607 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2209 2209 Size: 6085 SalI G'TCGA_C Cuts at: 1936 2579 3427 1936 Size: 643 848 4594 Fragments arranged by size: 4594 848 643 SbfI CC_TGCA'GG Cuts at: 3437 3437 Size: 6085 ScaI AGT'ACT Cuts at: 3010 4333 6045 3010 Size: 1323 1712 3050 Fragments arranged by size: 3050 1712 1323 SfcI C'TryA_G Cuts at: 826 1045 2840 3433 4568 5249 5440 826 Size: 219 1795 593 1135 681 191 1471 Fragments arranged by size: 1795 1471 1135 681 593 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 2013 3412 1872 Size: 141 1399 4545 Fragments arranged by size: 4545 1399 141 SmlI C'TyrA_G Cuts at: 820 1017 1943 2718 4189 5090 5352 820 Size: 197 926 775 1471 901 262 1553 Fragments arranged by size: 1553 1471 926 901 775 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 3184 4009 5 Size: 47 3132 825 2081 Fragments arranged by size: 3132 2081 825 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6085 StyI C'CwwG_G Cuts at: 513 1065 2004 3524 513 Size: 552 939 1520 3074 Fragments arranged by size: 3074 1520 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2352 4112 4129 4282 4467 4912 325 Size: 1255 772 1760 17 153 185 445 1498 Fragments arranged by size: 1760 1498 1255 772 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3008 4331 6043 Size: 274 80 33 51 1443 1031 1323 1712 Cuts at: 6043 96 Size: 138 Fragments arranged by size: 1712 1443 1323 1031 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3045 3077 6045 6077 3045 Size: 32 2968 32 3053 Fragments arranged by size: 3053 2968 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2373 1316 Size: 1057 5028 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 2011 3410 1870 Size: 141 1399 4545 Fragments arranged by size: 4545 1399 141 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3893 276 Size: 53 83 186 936 2359 2468 Fragments arranged by size: 2468 2359 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI