################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI ApaI AscI AseI AsnI Asp700I Asp718I AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI StuI StyI SwaI Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1918 AAATTC [GA]^AATT[CT] AhdI 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1416 GTGCAC G^TGCAC Aor51HI 965 AGCGCT AGC^GCT ApaLI 1416 GTGCAC G^TGCAC ApoI 1918 AAATTC [GA]^AATT[CT] AspEI 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1781 TGCGCA TGC^GCA BglI 1321 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1384 TACGTG [CT]AC^GT[GA] BsaI 165 GGTCTC GGTCTC(1/5) BsiEI 1443 CGACCG CG[GA][CT]^CG Bsp1407I 317 TGTACA T^GTACA BspLU11I 1682 ACATGT A^CATGT BsrGI 317 TGTACA T^GTACA BstYI 1007 GGATCT [GA]^GATC[CT] CpoI 1540 CGGACCG CG^G[AT]CCG DraIII 340 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1468 GACCCGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1844 TGGCCG [CT]^GGCC[GA] Eam1105I 1207 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 965 AGCGCT AGC^GCT EcoNI 1393 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 873 ATGCAT ATGCA^T FspI 1781 TGCGCA TGC^GCA HaeII 965 AGCGCT [GA]GCGC^[CT] MflI 1007 GGATCT [GA]^GATC[CT] NaeI 1247 GCCGGC GCC^GGC NgoMIV 1247 GCCGGC G^CCGGC NsiI 873 ATGCAT ATGCA^T PflFI 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC RsrII 1540 CGGACCG CG^G[AT]CCG SacI 1576 GAGCTC GAGCT^C SbfI 553 CCTGCAGG CCTGCA^GG SmaI 440 CCCGGG CCC^GGG SmlI 1121 CTCAAG C^T[CT][GA]AG SphI 923 GCATGC GCATG^C Sse8387I 553 CCTGCAGG CCTGCA^GG SspBI 317 TGTACA T^GTACA TaqI 502 TCGA T^CGA Tth111I 678 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 502 TCGA T^CGA XcmI 152 CCAGCAATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1007 GGATCT [GA]^GATC[CT] XmaCI 440 CCCGGG C^CCGGG XmaI 440 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 504 GACGTC GACGT^C 823 GACGTC AcyI 504 GACGTC G[GA]^CG[CT]C 823 GACGTC AocI 96 CCTGAGG CC^T[ACGT]AGG 407 CCTCAGG AspHI 1416 GTGCAC G[AT]GC[AT]^C 1576 GAGCTC BbvCI 656 CCTCAGC CCTCAGC(-5/-2) 1483 CCTCAGC BsaHI 504 GACGTC G[GA]^CG[CT]C 823 GACGTC BsaWI 333 ACCGGA [AT]^CCGG[AT] 1445 ACCGGA BsiHKAI 1416 GTGCAC G[AT]GC[AT]^C 1576 GAGCTC BsmBI 126 GAGACG CGTCTC(1/5) 862 GAGACG BsrBI 4 GAGCGG CCGCTC(-3/-3) 1003 GAGCGG BsrFI 1247 GCCGGC [GA]^CCGG[CT] 1543 ACCGGC BstAPI 875 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1418 GCACCTGCTGC Bsu36I 96 CCTGAGG CC^T[ACGT]AGG 407 CCTCAGG Cfr10I 1247 GCCGGC [GA]^CCGG[CT] 1543 ACCGGC DsaI 539 CCACGG C^C[GA][CT]GG 998 CCGTGG EarI 514 CTCTTC CTCTTC(1/4) 534 CTCTTC Eco81I 96 CCTGAGG CC^T[ACGT]AGG 407 CCTCAGG Hin1I 504 GACGTC G[GA]^CG[CT]C 823 GACGTC HincII 1204 GTTGAC GT[CT]^[GA]AC 1686 GTTGAC HindII 1204 GTTGAC GT[CT]^[GA]AC 1686 GTTGAC Ksp632I 514 CTCTTC CTCTTC(1/4) 534 CTCTTC TfiI 361 GAATC G^A[AT]TC 394 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI