(Circular) (Six-base) MAPSORT of: pFN21AB9944.seq Check: 8475 from: 1 to: 6661 pFN21AB9944 6661 bp With 182 enzymes: SgfI * March 28, 2012 12:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2411 3434 2411 Size: 1023 5638 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2513 2832 4471 278 Size: 53 83 186 936 977 319 1639 2468 Fragments arranged by size: 2468 1639 977 936 319 186 83 53 AccI GT'mk_AC Cuts at: 1937 4004 1937 Size: 2067 4594 Acc65I G'GTAC_C Cuts at: 3982 3982 Size: 6661 AclI AA'CG_TT Cuts at: 1563 3960 4788 5161 1563 Size: 2397 828 373 3063 Fragments arranged by size: 3063 2397 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 3668 4724 6108 6376 1632 Size: 653 1383 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1383 1056 653 268 AfeI AGC'GCT Cuts at: 2972 2972 Size: 6661 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 2934 3390 3687 5560 6537 1203 Size: 1731 456 297 1873 977 1327 Fragments arranged by size: 1873 1731 1327 977 456 297 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 3217 5390 3217 Size: 2173 4488 AleI CACnn'nnGTG Cuts at: 1166 2474 3899 1166 Size: 1308 1425 3928 Fragments arranged by size: 3928 1425 1308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6661 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3747 3779 3747 Size: 32 6629 AlwNI CAG_nnn'CTG Cuts at: 2843 2896 3337 4404 5976 2843 Size: 53 441 1067 1572 3528 Fragments arranged by size: 3528 1572 1067 441 53 ApaLI G'TGCA_C Cuts at: 3421 4718 5874 3421 Size: 1297 1156 4208 Fragments arranged by size: 4208 1297 1156 ApoI r'AATT_y Cuts at: 1493 3923 3970 4196 4348 6457 1493 Size: 2430 47 226 152 2109 1697 Fragments arranged by size: 2430 2109 1697 226 152 47 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2445 2956 3308 3986 1870 Size: 73 72 430 511 352 678 4545 Fragments arranged by size: 4545 678 511 430 352 73 72 BamHI G'GATC_C Cuts at: 3991 4358 3991 Size: 367 6294 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3982 5437 618 Size: 325 217 627 2195 1455 1842 Fragments arranged by size: 2195 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2733 3328 3585 3980 1391 Size: 1342 595 257 395 4072 Fragments arranged by size: 4072 1342 595 395 257 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5690 BbvCI CC'TCA_GC Cuts at: 2662 3489 2662 Size: 827 5834 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4852 4886 1903 Size: 34 2915 34 3678 Fragments arranged by size: 3678 2915 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BfrBI ATG'CAT Cuts at: 2880 2880 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3332 5272 136 Size: 107 122 71 631 605 1660 1940 1525 Fragments arranged by size: 1940 1660 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6656 6656 Size: 6661 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 3398 3425 4722 5878 1790 Size: 1608 27 1297 1156 2573 Fragments arranged by size: 2573 1608 1297 1156 27 BmgBI CAC'GTC Cuts at: 1318 2095 3357 1318 Size: 777 1262 4622 Fragments arranged by size: 4622 1262 777 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2310 3541 3886 Size: 416 53 83 399 24 893 1231 345 Cuts at: 3886 4392 5350 442 Size: 506 958 1753 Fragments arranged by size: 1753 1231 958 893 506 416 399 345 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2593 2593 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2196 2563 2635 3215 3698 5321 1239 Size: 957 367 72 580 483 1623 2579 Fragments arranged by size: 2579 1623 957 580 483 367 72 Bpu10I CC'TnA_GC Cuts at: 2662 3489 2662 Size: 827 5834 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3111 4786 5651 5949 3111 Size: 1675 865 298 3823 Fragments arranged by size: 3823 1675 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2176 5324 882 Size: 617 677 3148 2219 Fragments arranged by size: 3148 2219 677 617 BsaAI yAC'GTr Cuts at: 493 1204 3391 4452 493 Size: 711 2187 1061 2702 Fragments arranged by size: 2702 2187 1061 711 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 2829 4468 4850 Size: 53 83 186 936 977 319 1639 382 Cuts at: 4850 5485 275 Size: 635 1451 Fragments arranged by size: 1639 1451 977 936 635 382 319 186 83 53 BsaWI w'CCGG_w Cuts at: 2338 3450 5094 5492 5766 5913 6324 2338 Size: 1112 1644 398 274 147 411 2675 Fragments arranged by size: 2675 1644 1112 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2122 2152 2960 2990 3747 Size: 30 165 30 719 30 808 30 757 Cuts at: 3747 3777 1178 Size: 30 4062 Fragments arranged by size: 4062 808 757 719 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2766 2879 1186 Size: 907 673 113 4968 Fragments arranged by size: 4968 907 673 113 BseYI C'CCAG_C Cuts at: 2156 2843 3301 5864 6360 2156 Size: 687 458 2563 496 2457 Fragments arranged by size: 2563 2457 687 496 458 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2439 2445 2885 3523 1163 Size: 573 703 6 440 638 4301 Fragments arranged by size: 4301 703 638 573 440 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4111 4872 5900 1540 Size: 275 186 1450 660 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 660 275 186 BsiHKAI G_wGCw'C Cuts at: 3425 3585 4722 4807 5878 3425 Size: 160 1137 85 1071 4208 Fragments arranged by size: 4208 1137 1071 160 85 BsmI GAATG_Cn' Cuts at: 4172 4265 4172 Size: 93 6568 BsmBI CGTCTCn'nnnn_ Cuts at: 2125 2861 2125 Size: 736 5925 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2733 3328 3398 3425 3585 3980 4722 Size: 399 943 595 70 27 160 395 742 Cuts at: 4722 4807 5878 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 943 742 595 399 395 160 85 70 27 BspEI T'CCGG_A Cuts at: 6324 6324 Size: 6661 BspHI T'CATG_A Cuts at: 4550 6280 4550 Size: 1730 4931 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 2904 2999 3198 3434 4016 844 Size: 1567 493 95 199 236 582 3489 Fragments arranged by size: 3489 1567 582 493 236 199 95 BsrBI CCG'CTC Cuts at: 1394 1823 2011 3010 4548 1394 Size: 429 188 999 1538 3507 Fragments arranged by size: 3507 1538 999 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 5156 5330 65 Size: 1183 3908 174 1396 Fragments arranged by size: 3908 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3252 3548 5305 5492 1700 Size: 110 1442 296 1757 187 2869 Fragments arranged by size: 2869 1757 1442 296 187 110 BsrGI T'GTAC_A Cuts at: 96 2322 96 Size: 2226 4435 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6661 BssSI C'ACGA_G Cuts at: 2822 3041 3704 4721 5733 2822 Size: 219 663 1017 1012 3750 Fragments arranged by size: 3750 1017 1012 663 219 BstAPI GCAn_nnn'nTGC Cuts at: 794 2886 3429 794 Size: 2092 543 4026 Fragments arranged by size: 4026 2092 543 BstBI TT'CG_AA Cuts at: 5465 5465 Size: 6661 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6661 BstYI r'GATC_y Cuts at: 1972 3012 3991 4358 4743 4760 6212 6298 6310 Size: 1040 979 367 385 17 1452 86 12 Cuts at: 6310 6656 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1040 979 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2102 2413 2102 Size: 311 6350 BtgI C'CryG_G Cuts at: 513 1065 2004 2544 3003 513 Size: 552 939 540 459 4171 Fragments arranged by size: 4171 939 552 540 459 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5922 Fragments arranged by size: 5922 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3263 3295 3500 4177 4971 4991 3088 Size: 175 32 205 677 794 20 4758 Fragments arranged by size: 4758 794 677 205 175 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 3965 3965 Size: 6661 DraIII CAC_nnn'GTG Cuts at: 2350 2350 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3479 5668 809 Size: 507 2163 2189 1802 Fragments arranged by size: 2189 2163 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3849 4108 4997 Size: 54 1425 50 275 18 2019 259 889 Cuts at: 4997 6626 8 Size: 1629 43 Fragments arranged by size: 2019 1629 1425 889 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2525 2545 4591 1353 Size: 1172 20 2046 3423 Fragments arranged by size: 3423 2046 1172 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2143 5248 5622 5768 387 Size: 1064 138 554 3105 374 146 1280 Fragments arranged by size: 3105 1280 1064 554 374 146 138 EcoICRI GAG'CTC Cuts at: 3583 3583 Size: 6661 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2196 2285 2563 2635 3215 3668 3698 Size: 393 564 89 278 72 580 453 30 Cuts at: 3698 4724 5321 6108 6376 1239 Size: 1026 597 787 268 1524 Fragments arranged by size: 1524 1026 787 597 580 564 453 393 278 268 89 72 30 EcoNI CCTnn'n_nnAGG Cuts at: 3402 3402 Size: 6661 EcoO109I rG'GnC_Cy Cuts at: 2449 2761 2893 3187 3334 3472 2449 Size: 312 132 294 147 138 5638 Fragments arranged by size: 5638 312 294 147 138 132 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 3788 5167 3788 Size: 1379 5282 HaeII r_GCGC'y Cuts at: 1678 2974 5808 1678 Size: 1296 2834 2531 Fragments arranged by size: 2834 2531 1296 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2633 2665 2960 Size: 32 135 32 620 32 409 32 295 Cuts at: 2960 2992 3203 3235 3335 3367 5302 5334 5376 Size: 32 211 32 100 32 1935 32 42 Cuts at: 5376 5408 1373 Size: 32 2626 Fragments arranged by size: 2626 1935 620 409 295 211 135 100 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3211 3693 4005 4251 669 Size: 988 281 1273 482 312 246 3079 Fragments arranged by size: 3079 1273 988 482 312 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3986 3986 Size: 6661 MfeI C'AATT_G Cuts at: 4260 4260 Size: 6661 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2682 5775 5959 1305 Size: 1377 3093 184 2007 Fragments arranged by size: 3093 2007 1377 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4839 Fragments arranged by size: 4839 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 3899 3936 4619 4978 5137 518 Size: 648 1308 1425 37 683 359 159 2042 Fragments arranged by size: 2042 1425 1308 683 648 359 159 37 MspA1I CmG'CkG Cuts at: 1606 2840 2997 3718 4077 4754 5902 6147 1606 Size: 1234 157 721 359 677 1148 245 2120 Fragments arranged by size: 2120 1234 1148 721 677 359 245 157 NaeI GCC'GGC Cuts at: 3254 3254 Size: 6661 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5170 Fragments arranged by size: 5170 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6661 NgoMIV G'CCGG_C Cuts at: 3252 3252 Size: 6661 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NsiI A_TGCA'T Cuts at: 2882 2882 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2289 2880 2932 3136 3691 4019 5564 Size: 97 1060 591 52 204 555 328 1545 Cuts at: 5564 1132 Size: 2229 Fragments arranged by size: 2229 1545 1060 591 555 328 204 97 52 PasI CC'CwG_GG Cuts at: 1348 2049 3337 1348 Size: 701 1288 4672 Fragments arranged by size: 4672 1288 701 PciI A'CATG_T Cuts at: 3687 5560 3687 Size: 1873 4788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6661 PfoI T'CCnGG_A Cuts at: 3381 3381 Size: 6661 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3747 3779 4730 4762 6243 6275 3747 Size: 32 951 32 1481 32 4133 Fragments arranged by size: 4133 1481 951 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2449 2761 2893 3187 3472 2449 Size: 312 132 294 285 5638 Fragments arranged by size: 5638 312 294 285 132 PshAI GACnn'nnGTC Cuts at: 1613 3075 3377 3600 1613 Size: 1462 302 223 4674 Fragments arranged by size: 4674 1462 302 223 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PstI C_TGCA'G Cuts at: 830 2563 3141 3513 4013 830 Size: 1733 578 372 500 3478 Fragments arranged by size: 3478 1733 578 500 372 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 RsrII CG'GwC_CG Cuts at: 3546 3546 Size: 6661 SacI G_AGCT'C Cuts at: 3585 3585 Size: 6661 SalI G'TCGA_C Cuts at: 1936 4003 1936 Size: 2067 4594 SanDI GG'GwC_CC Cuts at: 2449 2449 Size: 6661 SbfI CC_TGCA'GG Cuts at: 2563 4013 2563 Size: 1450 5211 ScaI AGT'ACT Cuts at: 4909 6621 4909 Size: 1712 4949 SfcI C'TryA_G Cuts at: 826 1045 2185 2559 3137 3509 4009 5144 5825 Size: 219 1140 374 578 372 500 1135 681 Cuts at: 5825 6016 826 Size: 191 1471 Fragments arranged by size: 1471 1140 1135 681 578 500 374 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 2447 3988 1872 Size: 575 1541 4545 Fragments arranged by size: 4545 1541 575 SmlI C'TyrA_G Cuts at: 820 1017 1943 3126 4765 5666 5928 820 Size: 197 926 1183 1639 901 262 1553 Fragments arranged by size: 1639 1553 1183 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4452 493 Size: 3959 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 2932 4019 1132 Size: 1800 1087 3774 Fragments arranged by size: 3774 1800 1087 SspI AAT'ATT Cuts at: 5 52 4585 5 Size: 47 4533 2081 Fragments arranged by size: 4533 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6661 StyI C'CwwG_G Cuts at: 513 1065 2004 4100 513 Size: 552 939 2096 3074 Fragments arranged by size: 3074 2096 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3710 4688 4705 4858 5043 5488 Size: 1255 2001 129 978 17 153 185 445 Cuts at: 5488 325 Size: 1498 Fragments arranged by size: 2001 1498 1255 978 445 185 153 129 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3267 3528 Size: 274 80 33 51 1443 345 945 261 Cuts at: 3528 4907 6619 96 Size: 1379 1712 138 Fragments arranged by size: 1712 1443 1379 945 345 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3377 3409 6621 6653 3377 Size: 32 3212 32 3385 Fragments arranged by size: 3385 3212 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 1316 Size: 1370 5291 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 6661 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 2445 3986 1870 Size: 575 1541 4545 Fragments arranged by size: 4545 1541 575 XmnI GAAnn'nnTTC Cuts at: 4790 4790 Size: 6661 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2511 2830 4469 276 Size: 53 83 186 936 977 319 1639 2468 Fragments arranged by size: 2468 1639 977 936 319 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII SacII SapI SexAI SfiI SfoI SrfI SwaI