(Linear) (Six-base) MAPSORT of: KIBB9944.seq Check: 4067 from: 1 to: 1956 KIBB9944.orf With 182 enzymes: SgfI * March 22, 2012 15:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 406 1429 1956 Size: 406 1023 527 Fragments arranged by size: 1023 527 406 AatII G_ACGT'C Cuts at: 0 508 827 1956 Size: 508 319 1129 Fragments arranged by size: 1129 508 319 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 1663 1956 Size: 280 1383 293 Fragments arranged by size: 1383 293 280 AfeI AGC'GCT Cuts at: 0 967 1956 Size: 967 989 AflIII A'CryG_T Cuts at: 0 929 1385 1682 1956 Size: 929 456 297 274 Fragments arranged by size: 929 456 297 274 AhdI GACnn_n'nnGTC Cuts at: 0 1212 1956 Size: 1212 744 AleI CACnn'nnGTG Cuts at: 0 469 1894 1956 Size: 469 1425 62 Fragments arranged by size: 1425 469 62 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1742 1774 1956 Size: 1742 32 182 Fragments arranged by size: 1742 182 32 AlwNI CAG_nnn'CTG Cuts at: 0 838 891 1332 1956 Size: 838 53 441 624 Fragments arranged by size: 838 624 441 53 ApaLI G'TGCA_C Cuts at: 0 1416 1956 Size: 1416 540 ApoI r'AATT_y Cuts at: 0 1918 1956 Size: 1918 38 AvaI C'yCGr_G Cuts at: 0 10 440 951 1303 1956 Size: 10 430 511 352 653 Fragments arranged by size: 653 511 430 352 10 BanII G_rGCy'C Cuts at: 0 728 1323 1580 1956 Size: 728 595 257 376 Fragments arranged by size: 728 595 376 257 BbvCI CC'TCA_GC Cuts at: 0 657 1484 1956 Size: 657 827 472 Fragments arranged by size: 827 657 472 BfrBI ATG'CAT Cuts at: 0 875 1956 Size: 875 1081 BglI GCCn_nnn'nGGC Cuts at: 0 1327 1956 Size: 1327 629 Bme1580I G_kGCm'C Cuts at: 0 1393 1420 1956 Size: 1393 27 536 Fragments arranged by size: 1393 536 27 BmgBI CAC'GTC Cuts at: 0 90 1352 1956 Size: 90 1262 604 Fragments arranged by size: 1262 604 90 BmrI ACTGGGnnnn_n' Cuts at: 0 305 1536 1881 1956 Size: 305 1231 345 75 Fragments arranged by size: 1231 345 305 75 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 588 1956 Size: 588 1368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 191 558 630 1210 1693 1956 Size: 191 367 72 580 483 263 Fragments arranged by size: 580 483 367 263 191 72 Bpu10I CC'TnA_GC Cuts at: 0 657 1484 1956 Size: 657 827 472 Fragments arranged by size: 827 657 472 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1106 1956 Size: 1106 850 BsaI GGTCTCn'nnnn_ Cuts at: 0 171 1956 Size: 171 1785 BsaAI yAC'GTr Cuts at: 0 1386 1956 Size: 1386 570 BsaHI Gr'CG_yC Cuts at: 0 505 824 1956 Size: 505 319 1132 Fragments arranged by size: 1132 505 319 BsaWI w'CCGG_w Cuts at: 0 333 1445 1956 Size: 333 1112 511 Fragments arranged by size: 1112 511 333 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 117 147 955 985 1742 1772 1956 Size: 117 30 808 30 757 30 184 Fragments arranged by size: 808 757 184 117 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 761 874 1956 Size: 88 673 113 1082 Fragments arranged by size: 1082 673 113 88 BseYI C'CCAG_C Cuts at: 0 151 838 1296 1956 Size: 151 687 458 660 Fragments arranged by size: 687 660 458 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 434 440 880 1518 1956 Size: 434 6 440 638 438 Fragments arranged by size: 638 440 438 434 6 BsiEI CG_ry'CG Cuts at: 0 1446 1956 Size: 1446 510 BsiHKAI G_wGCw'C Cuts at: 0 1420 1580 1956 Size: 1420 160 376 Fragments arranged by size: 1420 376 160 BsmBI CGTCTCn'nnnn_ Cuts at: 0 120 856 1956 Size: 120 736 1100 Fragments arranged by size: 1100 736 120 Bsp1286I G_dGCh'C Cuts at: 0 728 1323 1393 1420 1580 1956 Size: 728 595 70 27 160 376 Fragments arranged by size: 728 595 376 160 70 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 406 899 994 1193 1429 1956 Size: 406 493 95 199 236 527 Fragments arranged by size: 527 493 406 236 199 95 BsrBI CCG'CTC Cuts at: 0 6 1005 1956 Size: 6 999 951 Fragments arranged by size: 999 951 6 BsrFI r'CCGG_y Cuts at: 0 1247 1543 1956 Size: 1247 296 413 Fragments arranged by size: 1247 413 296 BsrGI T'GTAC_A Cuts at: 0 317 1956 Size: 317 1639 BssSI C'ACGA_G Cuts at: 0 817 1036 1699 1956 Size: 817 219 663 257 Fragments arranged by size: 817 663 257 219 BstAPI GCAn_nnn'nTGC Cuts at: 0 881 1424 1956 Size: 881 543 532 Fragments arranged by size: 881 543 532 BstYI r'GATC_y Cuts at: 0 1007 1956 Size: 1007 949 Bsu36I CC'TnA_GG Cuts at: 0 97 408 1956 Size: 97 311 1548 Fragments arranged by size: 1548 311 97 BtgI C'CryG_G Cuts at: 0 539 998 1956 Size: 539 459 958 Fragments arranged by size: 958 539 459 BtsI GCAGTG_nn' Cuts at: 0 1083 1258 1290 1495 1956 Size: 1083 175 32 205 461 Fragments arranged by size: 1083 461 205 175 32 DraIII CAC_nnn'GTG Cuts at: 0 345 1956 Size: 345 1611 DrdI GACnn_nn'nnGTC Cuts at: 0 1474 1956 Size: 1474 482 EaeI y'GGCC_r Cuts at: 0 1844 1956 Size: 1844 112 EarI CTCTTCn'nnn_ Cuts at: 0 520 540 1956 Size: 520 20 1416 Fragments arranged by size: 1416 520 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 138 1956 Size: 138 1818 EcoICRI GAG'CTC Cuts at: 0 1578 1956 Size: 1578 378 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 191 280 558 630 1210 1663 1693 1956 Size: 191 89 278 72 580 453 30 263 Fragments arranged by size: 580 453 278 263 191 89 72 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1397 1956 Size: 1397 559 EcoO109I rG'GnC_Cy Cuts at: 0 444 756 888 1182 1329 1467 1956 Size: 444 312 132 294 147 138 489 Fragments arranged by size: 489 444 312 294 147 138 132 FspI TGC'GCA Cuts at: 0 1783 1956 Size: 1783 173 HaeII r_GCGC'y Cuts at: 0 969 1956 Size: 969 987 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 628 660 955 987 1198 1230 Size: 187 32 409 32 295 32 211 32 Cuts at: 1230 1330 1362 1956 Size: 100 32 594 Fragments arranged by size: 594 409 295 211 187 100 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1206 1688 1956 Size: 1206 482 268 Fragments arranged by size: 1206 482 268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 677 1956 Size: 677 1279 MslI CAynn'nnrTG Cuts at: 0 469 1894 1931 1956 Size: 469 1425 37 25 Fragments arranged by size: 1425 469 37 25 MspA1I CmG'CkG Cuts at: 0 835 992 1713 1956 Size: 835 157 721 243 Fragments arranged by size: 835 721 243 157 NaeI GCC'GGC Cuts at: 0 1249 1956 Size: 1249 707 NgoMIV G'CCGG_C Cuts at: 0 1247 1956 Size: 1247 709 NsiI A_TGCA'T Cuts at: 0 877 1956 Size: 877 1079 NspI r_CATG'y Cuts at: 0 284 875 927 1131 1686 1956 Size: 284 591 52 204 555 270 Fragments arranged by size: 591 555 284 270 204 52 PasI CC'CwG_GG Cuts at: 0 44 1332 1956 Size: 44 1288 624 Fragments arranged by size: 1288 624 44 PciI A'CATG_T Cuts at: 0 1682 1956 Size: 1682 274 PfoI T'CCnGG_A Cuts at: 0 1376 1956 Size: 1376 580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1742 1774 1956 Size: 1742 32 182 Fragments arranged by size: 1742 182 32 PpuMI rG'GwC_Cy Cuts at: 0 444 756 888 1182 1467 1956 Size: 444 312 132 294 285 489 Fragments arranged by size: 489 444 312 294 285 132 PshAI GACnn'nnGTC Cuts at: 0 1070 1372 1595 1956 Size: 1070 302 223 361 Fragments arranged by size: 1070 361 302 223 PstI C_TGCA'G Cuts at: 0 558 1136 1508 1956 Size: 558 578 372 448 Fragments arranged by size: 578 558 448 372 RsrII CG'GwC_CG Cuts at: 0 1541 1956 Size: 1541 415 SacI G_AGCT'C Cuts at: 0 1580 1956 Size: 1580 376 SanDI GG'GwC_CC Cuts at: 0 444 1956 Size: 444 1512 SbfI CC_TGCA'GG Cuts at: 0 558 1956 Size: 558 1398 SfcI C'TryA_G Cuts at: 0 180 554 1132 1504 1956 Size: 180 374 578 372 452 Fragments arranged by size: 578 452 374 372 180 SmaI CCC'GGG Cuts at: 0 442 1956 Size: 442 1514 SmlI C'TyrA_G Cuts at: 0 1121 1956 Size: 1121 835 SphI G_CATG'C Cuts at: 0 927 1956 Size: 927 1029 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1576 1705 1956 Size: 1576 129 251 Fragments arranged by size: 1576 251 129 TatI w'GTAC_w Cuts at: 0 317 1262 1523 1956 Size: 317 945 261 433 Fragments arranged by size: 945 433 317 261 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1372 1404 1956 Size: 1372 32 552 Fragments arranged by size: 1372 552 32 Tth111I GACn'n_nGTC Cuts at: 0 681 1956 Size: 681 1275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 159 1956 Size: 159 1797 XmaI C'CCGG_G Cuts at: 0 440 1956 Size: 440 1516 ZraI GAC'GTC Cuts at: 0 506 825 1956 Size: 506 319 1131 Fragments arranged by size: 1131 506 319 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaLI ApoI AvaI BanII BbvCI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PciI PfoI PpiI PpuMI PshAI PstI RsrII SacI SanDI SbfI SfcI SmaI SmlI SphI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI BclI BglII BlpI BmtI BsaBI BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI PacI PflMI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI PvuII SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI SwaI XbaI XhoI XmnI