Restriction map for
pFN21AE6318
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbeI BbsI BcgI BciVI BfaI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HaeII HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MroI MseI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PleI PmeI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 286 GTCTAC GT^[AC][GT]AC
ApaI 1093 GGGCCC GGGCC^C
AviII 902 TGCGCA TGC^GCA
BalI 368 TGGCCA TGG^CCA
BamHI 88 GGATCC G^GATCC
BclI 683 TGATCA T^GATCA
BlpI 978 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 978 GCTGAGC GC^T[ACGT]AGC
BsaI 493 GAGACC GGTCTC(1/5)
BsaWI 85 ACCGGA [AT]^CCGG[AT]
BsmAI 493 GAGAC GTCTC(1/5)
BspHI 513 TCATGA T^CATGA
BssSI 244 CACGAG CACGAG(-5/-1)
CelII 978 GCTGAGC GC^T[ACGT]AGC
FbaI 683 TGATCA T^GATCA
FspI 902 TGCGCA TGC^GCA
HphI 83 TCACC GGTGA(8/7)
MluNI 368 TGGCCA TGG^CCA
MscI 368 TGGCCA TGG^CCA
PaeR7I 205 CTCGAG C^TCGAG
PshAI 148 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 513 TCATGA T^CATGA
SacI 67 GAGCTC GAGCT^C
SapI 561 GCTCTTC GCTCTTC(1/4)
ScaI 734 AGTACT AGT^ACT
SmaI 812 CCCGGG CCC^GGG
SmlI 205 CTCGAG C^T[CT][GA]AG
XhoI 205 CTCGAG C^TCGAG
XmaCI 812 CCCGGG C^CCGGG
XmaI 812 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AfaI 600 GTAC GT^AC
735 GTAC
Alw44I 241 GTGCAC G^TGCAC
352 GTGCAC
AocI 309 CCTGAGG CC^T[ACGT]AGG
621 CCTAAGG
ApaLI 241 GTGCAC G^TGCAC
352 GTGCAC
BbrPI 349 CACGTG CAC^GTG
357 CACGTG
BbvCI 430 CCTCAGC CCTCAGC(-5/-2)
912 GCTGAGG
BsgI 49 CTGCAC GTGCAG(16/14)
658 GTGCAG
BsmFI 300 GTCCC GGGAC(10/14)
1128 GTCCC
BspLU11I 194 ACATGT A^CATGT
374 ACATGT
BsrI 711 CCAGT ACTGG(1/-1)
758 CCAGT
BstAPI 1069 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1137 GCACTCGCTGC
BstYI 88 GGATCC [GA]^GATC[CT]
218 AGATCC
Bsu36I 309 CCTGAGG CC^T[ACGT]AGG
621 CCTAAGG
CfoI 903 GCGC GCG^C
963 GCGC
DraIII 244 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
339 CACAGAGTG
DsaI 543 CCACGG C^C[GA][CT]GG
649 CCGTGG
EaeI 251 TGGCCG [CT]^GGCC[GA]
368 TGGCCA
EarI 562 CTCTTC CTCTTC(1/4)
591 CTCTTC
Eco81I 309 CCTGAGG CC^T[ACGT]AGG
621 CCTAAGG
EcoT14I 638 CCAAGG C^C[AT][AT]GG
848 CCAAGG
HhaI 903 GCGC GCG^C
963 GCGC
HinP1I 903 GCGC G^CGC
963 GCGC
Ksp632I 562 CTCTTC CTCTTC(1/4)
591 CTCTTC
MflI 88 GGATCC [GA]^GATC[CT]
218 AGATCC
MslI 234 CATGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
754 CACACCAGTG
PflMI 338 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCACACGCTGG
PmaCI 349 CACGTG CAC^GTG
357 CACGTG
PmlI 349 CACGTG CAC^GTG
357 CACGTG
PpuMI 332 AGGACCC [GA]G^G[AT]CC[CT]
404 AGGACCT
RsaI 600 GTAC GT^AC
735 GTAC
StuI 587 AGGCCT AGG^CCT
794 AGGCCT
StyI 638 CCAAGG C^C[AT][AT]GG
848 CCAAGG
TaqI 206 TCGA T^CGA
452 TCGA
TthHB8I 206 TCGA T^CGA
452 TCGA
Van91I 338 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
387 CCACACGCTGG
XhoII 88 GGATCC [GA]^GATC[CT]
218 AGATCC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AflIII AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BspMI BsrBI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MaeII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
TspRI