Restriction map for
pFN21AE6318
(Circular) (Six-base) MAPSORT of: pFN21AE6318.seq Check: 5114 from: 1 to: 5857
pFN21AE6318 5857 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2155 3064 2155
Size: 909 4948
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3667 278
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 3200 1937
Size: 355 908 4594
Fragments arranged by size:
4594 908 355
Acc65I G'GTAC_C
Cuts at: 3178 3178
Size: 5857
AclI AA'CG_TT
Cuts at: 1563 3984 4357 1563
Size: 2421 373 3063
Fragments arranged by size:
3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 2747 3920 5304 5572 1632
Size: 1061 54 1173 1384 268 1917
Fragments arranged by size:
1917 1384 1173 1061 268 54
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5660
AflIII A'CryG_T
Cuts at: 1203 2199 2361 2379 4756 5733 1203
Size: 996 162 18 2377 977 1327
Fragments arranged by size:
2377 1327 996 977 162 18
AgeI A'CCGG_T
Cuts at: 4688 4688
Size: 5857
AhdI GACnn_n'nnGTC
Cuts at: 4586 4586
Size: 5857
AleI CACnn'nnGTG
Cuts at: 1166 2763 1166
Size: 1597 4260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2490 1184
Size: 1306 4551
AlwNI CAG_nnn'CTG
Cuts at: 2443 2671 2722 3600 5172 2443
Size: 228 51 878 1572 3128
Fragments arranged by size:
3128 1572 878 228 51
ApaI G_GGCC'C
Cuts at: 3102 3102
Size: 5857
ApaLI G'TGCA_C
Cuts at: 2246 2357 3914 5070 2246
Size: 111 1557 1156 3033
Fragments arranged by size:
3033 1557 1156 111
ApoI r'AATT_y
Cuts at: 1493 3166 3392 3544 5653 1493
Size: 1673 226 152 2109 1697
Fragments arranged by size:
2109 1697 1673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4411 160
Size: 872 3379 1606
Fragments arranged by size:
3379 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
AvaI C'yCGr_G
Cuts at: 1870 1943 2097 2210 2479 2817 3182 1870
Size: 73 154 113 269 338 365 4545
Fragments arranged by size:
4545 365 338 269 154 113 73
BamHI G'GATC_C
Cuts at: 2093 3187 3554 2093
Size: 1094 367 4396
Fragments arranged by size:
4396 1094 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2536 2778 2993 3178 4633
Size: 325 217 627 749 242 215 185 1455
Cuts at: 4633 618
Size: 1842
Fragments arranged by size:
1842 1455 749 627 325 242 217 215
185
BanII G_rGCy'C
Cuts at: 1391 2076 2824 3102 3176 1391
Size: 685 748 278 74 4072
Fragments arranged by size:
4072 748 685 278 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4886
BbvCI CC'TCA_GC
Cuts at: 2436 2918 2436
Size: 482 5375
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4048 4082 1903
Size: 34 2111 34 3678
Fragments arranged by size:
3678 2111 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3749 4959 3749
Size: 1210 4647
BclI T'GATC_A
Cuts at: 1548 2688 1548
Size: 1140 4717
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4468 136
Size: 107 122 71 631 605 2796 1525
Fragments arranged by size:
2796 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5852 5852
Size: 5857
BlpI GC'TnA_GC
Cuts at: 2984 3274 2984
Size: 290 5567
Bme1580I G_kGCm'C
Cuts at: 1790 2066 2250 2361 2541 2818 3102 3144 3918
Size: 276 184 111 180 277 284 42 774
Cuts at: 3918 5074 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 774 284 277 276 184 180
111 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5857
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3588 4546 442
Size: 416 53 83 399 24 2171 958 1753
Fragments arranged by size:
2171 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5857
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 5857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2699 2774 2849 2897 2903 2948 4517 1239
Size: 1460 75 75 48 6 45 1569 2579
Fragments arranged by size:
2579 1569 1460 75 75 48 45 6
Bpu10I CC'TnA_GC
Cuts at: 2436 2510 2918 2436
Size: 74 408 5375
Fragments arranged by size:
5375 408 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3982 4847 5145 3982
Size: 865 298 4694
Fragments arranged by size:
4694 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2492 4520 882
Size: 617 993 2028 2219
Fragments arranged by size:
2219 2028 993 617
BsaAI yAC'GTr
Cuts at: 493 1204 2356 2364 2809 3648 493
Size: 711 1152 8 445 839 2702
Fragments arranged by size:
2702 1152 839 711 445 8
BsaBI GATnn'nnATC
Cuts at: 5851 5851
Size: 5857
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3664 4046 4681 275
Size: 53 83 186 936 2131 382 635 1451
Fragments arranged by size:
2131 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2090 4290 4688 4962 5109 5520 2090
Size: 2200 398 274 147 411 2427
Fragments arranged by size:
2427 2200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2651 2669 2681 2989
Size: 30 165 30 1236 12 18 12 308
Cuts at: 2989 3019 1178
Size: 30 4016
Fragments arranged by size:
4016 1236 308 165 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2603 2750 2969 2972 1186
Size: 964 453 147 219 3 4071
Fragments arranged by size:
4071 964 453 219 147 3
BseYI C'CCAG_C
Cuts at: 2398 2575 5060 5556 2398
Size: 177 2485 496 2699
Fragments arranged by size:
2699 2485 496 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 2684 1163
Size: 573 303 645 4336
Fragments arranged by size:
4336 645 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3307 4068 5096 1540
Size: 275 186 1306 761 1028 2301
Fragments arranged by size:
2301 1306 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 2250 2361 2760 3918 4003 5074 2076
Size: 174 111 399 1158 85 1071 2859
Fragments arranged by size:
2859 1158 1071 399 174 111 85
BsmI GAATG_Cn'
Cuts at: 3368 3461 3368
Size: 93 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2076 2250 2361 2541 2760 2818
Size: 399 276 10 174 111 180 219 58
Cuts at: 2818 2824 3102 3144 3176 3918 4003 5074 1391
Size: 6 278 42 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 278 276 219 180
174 111 85 58 42 32 10 6
BspEI T'CCGG_A
Cuts at: 5520 5520
Size: 5857
BspHI T'CATG_A
Cuts at: 2518 3746 5476 2518
Size: 1228 1730 2899
Fragments arranged by size:
2899 1730 1228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2155 2458 2643 3064 3212 844
Size: 1311 303 185 421 148 3489
Fragments arranged by size:
3489 1311 421 303 185 148
BsrBI CCG'CTC
Cuts at: 1394 1823 2015 2140 2962 3744 1394
Size: 429 192 125 822 782 3507
Fragments arranged by size:
3507 822 782 429 192 125
BsrDI GCAATG_nn'
Cuts at: 65 1248 4352 4526 65
Size: 1183 3104 174 1396
Fragments arranged by size:
3104 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4501 4688 1700
Size: 110 2691 187 2869
Fragments arranged by size:
2869 2691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5857
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5857
BssSI C'ACGA_G
Cuts at: 2249 3917 4929 2249
Size: 1668 1012 3177
Fragments arranged by size:
3177 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3080 3148 794
Size: 2286 68 3503
Fragments arranged by size:
3503 2286 68
BstBI TT'CG_AA
Cuts at: 4661 4661
Size: 5857
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5857
BstYI r'GATC_y
Cuts at: 1972 2093 2223 3187 3554 3939 3956 5408 5494
Size: 121 130 964 367 385 17 1452 86
Cuts at: 5494 5506 5852 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 964 385 367 346 130 121
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2315 2627 2315
Size: 312 5545
BtgI C'CryG_G
Cuts at: 513 1065 2004 2548 2654 513
Size: 552 939 544 106 3716
Fragments arranged by size:
3716 939 552 544 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5118
Fragments arranged by size:
5118 683 56
BtsI GCAGTG_nn'
Cuts at: 2672 3373 4167 4187 2672
Size: 701 794 20 4342
Fragments arranged by size:
4342 794 701 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5857
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5822
DraI TTT'AAA
Cuts at: 3161 3161
Size: 5857
DraIII CAC_nnn'GTG
Cuts at: 2254 2349 2254
Size: 95 5762
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4864 809
Size: 507 3548 1802
Fragments arranged by size:
3548 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 2373 3304
Size: 54 1425 50 275 18 426 117 931
Cuts at: 3304 4193 5822 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 931 889 426 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3304 1537
Size: 275 1492 4090
Fragments arranged by size:
4090 1492 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2573 2602 3787 1353
Size: 1220 29 1185 3423
Fragments arranged by size:
3423 1220 1185 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2873 2957 4444 4818 4964 387
Size: 1064 138 1284 84 1487 374 146 1280
Fragments arranged by size:
1487 1284 1280 1064 374 146 138 84
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 5857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2693 2699 2747 2774 2849 2897 2903
Size: 393 1061 6 48 27 75 48 6
Cuts at: 2903 2948 3920 4517 5304 5572 1239
Size: 45 972 597 787 268 1524
Fragments arranged by size:
1524 1061 972 787 597 393 268 75
48 48 45 27 6 6
EcoO109I rG'GnC_Cy
Cuts at: 2338 2410 3098 3099 2338
Size: 72 688 1 5096
Fragments arranged by size:
5096 688 72 1
EcoRI G'AATT_C
Cuts at: 3166 3544 3166
Size: 378 5479
FspI TGC'GCA
Cuts at: 2909 4363 2909
Size: 1454 4403
HaeII r_GCGC'y
Cuts at: 1678 5004 1678
Size: 3326 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2680 2712 4498 4530 4572
Size: 32 135 32 1108 32 1786 32 42
Cuts at: 4572 4604 1373
Size: 32 2626
Fragments arranged by size:
2626 1786 1108 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3201 3447 669
Size: 988 281 1263 246 3079
Fragments arranged by size:
3079 1263 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5857
HpaI GTT'AAC
Cuts at: 3447 3447
Size: 5857
KpnI G_GTAC'C
Cuts at: 3182 3182
Size: 5857
MfeI C'AATT_G
Cuts at: 3456 3456
Size: 5857
MluI A'CGCG_T
Cuts at: 5733 5733
Size: 5857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4971 5155 1305
Size: 3666 184 2007
Fragments arranged by size:
3666 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3492
Fragments arranged by size:
3492 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2243 2763 3815 4174 4333 518
Size: 648 1077 520 1052 359 159 2042
Fragments arranged by size:
2042 1077 1052 648 520 359 159
MspA1I CmG'CkG
Cuts at: 1606 3273 3950 5098 5343 1606
Size: 1667 677 1148 245 2120
Fragments arranged by size:
2120 1667 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4366
Fragments arranged by size:
4366 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5857
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5857
NotI GC'GGCC_GC
Cuts at: 3304 3304
Size: 5857
NspI r_CATG'y
Cuts at: 1132 1229 2203 2383 2983 3215 4760 1132
Size: 97 974 180 600 232 1545 2229
Fragments arranged by size:
2229 1545 974 600 232 180 97
PasI CC'CwG_GG
Cuts at: 1348 2329 3137 1348
Size: 981 808 4068
Fragments arranged by size:
4068 981 808
PciI A'CATG_T
Cuts at: 2199 2379 4756 2199
Size: 180 2377 3300
Fragments arranged by size:
3300 2377 180
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2349 2398 1108
Size: 1241 49 4567
Fragments arranged by size:
4567 1241 49
PfoI T'CCnGG_A
Cuts at: 2306 2306
Size: 5857
PmeI GTTT'AAAC
Cuts at: 3161 3161
Size: 5857
PmlI CAC'GTG
Cuts at: 2356 2364 2356
Size: 8 5849
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3926 3958 5439 5471 3926
Size: 32 1481 32 4312
Fragments arranged by size:
4312 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2338 2410 2338
Size: 72 5785
PshAI GACnn'nnGTC
Cuts at: 1613 2157 1613
Size: 544 5313
PsiI TTA'TAA
Cuts at: 3427 3427
Size: 5857
PspOMI G'GGCC_C
Cuts at: 3098 3098
Size: 5857
PspXI vC'TCGA_Gb
Cuts at: 2210 2210
Size: 5857
PstI C_TGCA'G
Cuts at: 830 2427 2469 2640 3061 3085 3109 3153 3209
Size: 1597 42 171 421 24 24 44 56
Cuts at: 3209 830
Size: 3478
Fragments arranged by size:
3478 1597 421 171 56 44 42 24
24
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5857
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 5857
SalI G'TCGA_C
Cuts at: 1936 3199 1936
Size: 1263 4594
SapI GCTCTTCn'nnn_
Cuts at: 2573 2573
Size: 5857
SbfI CC_TGCA'GG
Cuts at: 3209 3209
Size: 5857
ScaI AGT'ACT
Cuts at: 2741 4105 5817 2741
Size: 1364 1712 2781
Fragments arranged by size:
2781 1712 1364
SfcI C'TryA_G
Cuts at: 826 1045 2423 2465 2636 3057 3081 3105 3149
Size: 219 1378 42 171 421 24 24 44
Cuts at: 3149 3205 4340 5021 5212 826
Size: 56 1135 681 191 1471
Fragments arranged by size:
1471 1378 1135 681 421 219 191 171
56 44 42 24 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5857
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5857
SmaI CCC'GGG
Cuts at: 1872 2819 3184 1872
Size: 947 365 4545
Fragments arranged by size:
4545 947 365
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 3961 4862 5124 820
Size: 197 926 267 1751 901 262 1553
Fragments arranged by size:
1751 1553 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3648 493
Size: 3155 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5857
SphI G_CATG'C
Cuts at: 1132 3215 1132
Size: 2083 3774
SrfI GCCC'GGGC
Cuts at: 2819 2819
Size: 5857
SspI AAT'ATT
Cuts at: 5 52 3781 5
Size: 47 3729 2081
Fragments arranged by size:
3729 2081 47
StuI AGG'CCT
Cuts at: 1517 2594 2801 1517
Size: 1077 207 4573
Fragments arranged by size:
4573 1077 207
StyI C'CwwG_G
Cuts at: 513 1065 2004 2643 2853 3296 513
Size: 552 939 639 210 443 3074
Fragments arranged by size:
3074 939 639 552 443 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2799 3034 3082 3884 3901 4054 4239
Size: 1255 1219 235 48 802 17 153 185
Cuts at: 4239 4684 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1219 802 445 235 185 153
48 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2739 4103 5815
Size: 274 80 33 51 1443 762 1364 1712
Cuts at: 5815 96
Size: 138
Fragments arranged by size:
1712 1443 1364 762 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2085 2117 2639 2651 2671 2683 2989 3021 5817
Size: 32 522 12 20 12 306 32 2796
Cuts at: 5817 5849 2085
Size: 32 2093
Fragments arranged by size:
2796 2093 522 306 32 32 32 20
12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5857
XbaI T'CTAG_A
Cuts at: 3193 3193
Size: 5857
XhoI C'TCGA_G
Cuts at: 1943 2210 1943
Size: 267 5590
XmaI C'CCGG_G
Cuts at: 1870 2817 3182 1870
Size: 947 365 4545
Fragments arranged by size:
4545 947 365
XmnI GAAnn'nnTTC
Cuts at: 3986 3986
Size: 5857
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3665 276
Size: 53 83 186 936 2131 2468
Fragments arranged by size:
2468 2131 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SwaI
XcmI