################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 673 CGCG CG^CG AcsI 574 AAATTC [GA]^AATT[CT] AcyI 199 GGCGCC G[GA]^CG[CT]C AfaI 514 GTAC GT^AC ApoI 574 AAATTC [GA]^AATT[CT] BanII 409 GAGCCC G[GA]GC[CT]^C BbeI 199 GGCGCC GGCGC^C BbsI 562 GTCTTC GAAGAC(2/6) BcnI 71 CCGGG CC^[GC]GG BmyI 409 GAGCCC G[AGT]GC[ACT]^C BpuAI 562 GTCTTC GAAGAC(2/6) BsaHI 199 GGCGCC G[GA]^CG[CT]C BsgI 244 GTGCAG GTGCAG(16/14) Bsp1286I 409 GAGCCC G[AGT]GC[ACT]^C BspMI 35 ACCTGC ACCTGC(4/8) BsrDI 606 CATTGC GCAATG(2/0) BsrI 255 CCAGT ACTGG(1/-1) BstAPI 17 GCACCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 673 CGCG CG^CG DraIII 18 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 611 CCGTGG C^C[GA][CT]GG EaeI 317 CGGCCA [CT]^GGCC[GA] EcoT14I 401 CCAAGG C^C[AT][AT]GG HaeII 199 GGCGCC [GA]GCGC^[CT] HaeIII 318 GGCC GG^CC Hin1I 199 GGCGCC G[GA]^CG[CT]C KasI 199 GGCGCC G^GCGCC MaeII 512 ACGT A^CGT MspA1I 415 CAGCGG C[AC]G^C[GT]G MvnI 673 CGCG CG^CG NaeI 196 GCCGGC GCC^GGC NarI 199 GGCGCC GG^CGCC NciI 71 CCGGG CC^[GC]GG NgoMIV 196 GCCGGC G^CCGGC NspI 260 GCATGC [GA]CATG^[CT] RsaI 514 GTAC GT^AC SfoI 199 GGCGCC GGC^GCC SphI 260 GCATGC GCATG^C StyI 401 CCAAGG C^C[AT][AT]GG TfiI 107 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanI 16 GGCACC G^G[CT][GA]CC 199 GGCGCC BsmFI 267 GGGAC GGGAC(10/14) 252 GTCCC BspHI 206 TCATGA T^CATGA 617 TCATGA BsrFI 3 GCCGGT [GA]^CCGG[CT] 196 GCCGGC BstNI 225 CCTGG CC^[AT]GG 363 CCAGG CfoI 200 GCGC GCG^C 590 GCGC Cfr10I 3 GCCGGT [GA]^CCGG[CT] 196 GCCGGC DdeI 116 CTCAG C^T[ACGT]AG 489 CTCAG EcoRII 225 CCTGG ^CC[AT]GG 363 CCAGG FauI 236 GCGGG CCCGC(4/6) 417 GCGGG HhaI 200 GCGC GCG^C 590 GCGC HinP1I 200 GCGC G^CGC 590 GCGC MseI 50 TTAA T^TAA 353 TTAA MslI 337 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 606 CATTGCCGTG MvaI 225 CCTGG CC^[AT]GG 363 CCAGG RcaI 206 TCATGA T^CATGA 617 TCATGA Tru9I 50 TTAA T^TAA 353 TTAA Tsp509I 157 AATT ^AATT 575 AATT TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^ 593 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BssKI BstF5I Cac8I DpnII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MboI MboII MnlI MspI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI TaqI TseI TthHB8I