(Circular) (Six-base) MAPSORT of: pFN21AE1546.seq Check: 8994 from: 1 to: 5389 pFN21AE1546 5389 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3199 278 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2732 1937 Size: 795 4594 Acc65I G'GTAC_C Cuts at: 2710 2710 Size: 5389 AclI AA'CG_TT Cuts at: 1563 3516 3889 1563 Size: 1953 373 3063 Fragments arranged by size: 3063 1953 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2626 3452 4836 5104 1632 Size: 994 826 1384 268 1917 Fragments arranged by size: 1917 1384 994 826 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5192 AflIII A'CryG_T Cuts at: 1203 4288 5265 1203 Size: 3085 977 1327 Fragments arranged by size: 3085 1327 977 AgeI A'CCGG_T Cuts at: 4220 4220 Size: 5389 AhdI GACnn_n'nnGTC Cuts at: 4118 4118 Size: 5389 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5389 AlwNI CAG_nnn'CTG Cuts at: 3132 4704 3132 Size: 1572 3817 ApaLI G'TGCA_C Cuts at: 3446 4602 3446 Size: 1156 4233 ApoI r'AATT_y Cuts at: 1493 2579 2698 2924 3076 5185 1493 Size: 1086 119 226 152 2109 1697 Fragments arranged by size: 2109 1697 1086 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 3943 160 Size: 872 2911 1606 Fragments arranged by size: 2911 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 AvaI C'yCGr_G Cuts at: 1870 1943 2714 1870 Size: 73 771 4545 Fragments arranged by size: 4545 771 73 BamHI G'GATC_C Cuts at: 2719 3086 2719 Size: 367 5022 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2204 2710 4165 618 Size: 325 217 627 234 183 506 1455 1842 Fragments arranged by size: 1842 1455 627 506 325 234 217 183 BanII G_rGCy'C Cuts at: 1391 2418 2708 1391 Size: 1027 290 4072 Fragments arranged by size: 4072 1027 290 BbeI G_GCGC'C Cuts at: 2208 2208 Size: 5389 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2560 928 Size: 971 661 3757 Fragments arranged by size: 3757 971 661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3580 3614 1903 Size: 34 50 34 1559 34 3678 Fragments arranged by size: 3678 1559 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3281 4491 3281 Size: 1210 4179 BclI T'GATC_A Cuts at: 1548 1548 Size: 5389 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4000 136 Size: 107 122 71 631 605 2328 1525 Fragments arranged by size: 2328 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5384 5384 Size: 5389 BlpI GC'TnA_GC Cuts at: 2806 2806 Size: 5389 Bme1580I G_kGCm'C Cuts at: 1790 3450 4606 1790 Size: 1660 1156 2573 Fragments arranged by size: 2573 1660 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5389 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 3120 4078 Size: 416 53 83 399 24 837 866 958 Cuts at: 4078 442 Size: 1753 Fragments arranged by size: 1753 958 866 837 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5389 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4049 1239 Size: 2810 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3514 4379 4677 3514 Size: 865 298 4226 Fragments arranged by size: 4226 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4052 882 Size: 617 2553 2219 Fragments arranged by size: 2553 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3180 493 Size: 711 1976 2702 Fragments arranged by size: 2702 1976 711 BsaBI GATnn'nnATC Cuts at: 5383 5383 Size: 5389 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2205 3196 3578 4213 Size: 53 83 186 936 672 991 382 635 Cuts at: 4213 275 Size: 1451 Fragments arranged by size: 1451 991 936 672 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3822 4220 4494 4641 5052 3822 Size: 398 274 147 411 4159 Fragments arranged by size: 4159 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2246 2276 1178 Size: 30 165 30 688 30 125 30 4291 Fragments arranged by size: 4291 688 165 125 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5389 BseYI C'CCAG_C Cuts at: 2269 2418 2471 4592 5088 2269 Size: 149 53 2121 496 2570 Fragments arranged by size: 2570 2121 496 149 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 1163 Size: 573 534 4282 Fragments arranged by size: 4282 573 534 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2839 3600 4628 1540 Size: 275 186 838 761 1028 2301 Fragments arranged by size: 2301 1028 838 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3450 3535 4606 3450 Size: 85 1071 4233 Fragments arranged by size: 4233 1071 85 BsmI GAATG_Cn' Cuts at: 2900 2993 2900 Size: 93 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2418 2708 3450 3535 4606 1391 Size: 399 628 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 628 399 290 85 BspEI T'CCGG_A Cuts at: 5052 5052 Size: 5389 BspHI T'CATG_A Cuts at: 2211 2622 3278 5008 2211 Size: 411 656 1730 2592 Fragments arranged by size: 2592 1730 656 411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2049 2744 844 Size: 1205 695 3489 Fragments arranged by size: 3489 1205 695 BsrBI CCG'CTC Cuts at: 1394 1823 3276 1394 Size: 429 1453 3507 Fragments arranged by size: 3507 1453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2610 3884 4058 65 Size: 1183 1362 1274 174 1396 Fragments arranged by size: 1396 1362 1274 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 2201 4033 4220 1700 Size: 110 198 193 1832 187 2869 Fragments arranged by size: 2869 1832 198 193 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5389 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5389 BssSI C'ACGA_G Cuts at: 3449 4461 3449 Size: 1012 4377 BstAPI GCAn_nnn'nTGC Cuts at: 794 2028 794 Size: 1234 4155 BstBI TT'CG_AA Cuts at: 4193 4193 Size: 5389 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5389 BstYI r'GATC_y Cuts at: 1972 2719 3086 3471 3488 4940 5026 5038 5384 Size: 747 367 385 17 1452 86 12 346 Cuts at: 5384 1972 Size: 1977 Fragments arranged by size: 1977 1452 747 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2616 513 Size: 552 1551 3286 Fragments arranged by size: 3286 1551 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4650 Fragments arranged by size: 4650 683 56 BtsI GCAGTG_nn' Cuts at: 2604 2905 3699 3719 2604 Size: 301 794 20 4274 Fragments arranged by size: 4274 794 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5354 DraI TTT'AAA Cuts at: 2693 2693 Size: 5389 DraIII CAC_nnn'GTG Cuts at: 2028 2028 Size: 5389 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4396 809 Size: 507 3080 1802 Fragments arranged by size: 3080 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2836 3725 Size: 54 1425 50 275 18 492 514 889 Cuts at: 3725 5354 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 514 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2836 1537 Size: 275 1024 4090 Fragments arranged by size: 4090 1024 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3319 1353 Size: 1966 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3976 4350 4496 387 Size: 1064 138 2387 374 146 1280 Fragments arranged by size: 2387 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2626 3452 4049 4836 5104 1239 Size: 393 994 826 597 787 268 1524 Fragments arranged by size: 1524 994 826 787 597 393 268 EcoRI G'AATT_C Cuts at: 2698 3076 2698 Size: 378 5011 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 5389 FspI TGC'GCA Cuts at: 3895 3895 Size: 5389 HaeII r_GCGC'y Cuts at: 1678 2208 4536 1678 Size: 530 2328 2531 Fragments arranged by size: 2531 2328 530 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 4030 4062 4104 Size: 32 135 32 519 32 1907 32 42 Cuts at: 4104 4136 1373 Size: 32 2626 Fragments arranged by size: 2626 1907 519 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2733 2979 669 Size: 988 281 795 246 3079 Fragments arranged by size: 3079 988 795 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5389 HpaI GTT'AAC Cuts at: 2979 2979 Size: 5389 KasI G'GCGC_C Cuts at: 2204 2204 Size: 5389 KpnI G_GTAC'C Cuts at: 2714 2714 Size: 5389 MfeI C'AATT_G Cuts at: 2988 2988 Size: 5389 MluI A'CGCG_T Cuts at: 5265 5265 Size: 5389 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4503 4687 1305 Size: 3198 184 2007 Fragments arranged by size: 3198 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3567 Fragments arranged by size: 3567 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2346 2615 3347 3706 3865 518 Size: 648 1180 269 732 359 159 2042 Fragments arranged by size: 2042 1180 732 648 359 269 159 MspA1I CmG'CkG Cuts at: 1606 2422 2805 3482 4630 4875 1606 Size: 816 383 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 383 245 NaeI GCC'GGC Cuts at: 2203 2203 Size: 5389 NarI GG'CG_CC Cuts at: 2205 2205 Size: 5389 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4837 NdeI CA'TA_TG Cuts at: 387 387 Size: 5389 NgoMIV G'CCGG_C Cuts at: 2201 2201 Size: 5389 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5389 NotI GC'GGCC_GC Cuts at: 2836 2836 Size: 5389 NspI r_CATG'y Cuts at: 1132 1229 2269 2747 4292 1132 Size: 97 1040 478 1545 2229 Fragments arranged by size: 2229 1545 1040 478 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5389 PciI A'CATG_T Cuts at: 4288 4288 Size: 5389 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5389 PfoI T'CCnGG_A Cuts at: 2367 2367 Size: 5389 PmeI GTTT'AAAC Cuts at: 2693 2693 Size: 5389 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3458 3490 4971 5003 3458 Size: 32 1481 32 3844 Fragments arranged by size: 3844 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5389 PsiI TTA'TAA Cuts at: 2959 2959 Size: 5389 PstI C_TGCA'G Cuts at: 830 2741 830 Size: 1911 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5389 SalI G'TCGA_C Cuts at: 1936 2731 1936 Size: 795 4594 SbfI CC_TGCA'GG Cuts at: 2741 2741 Size: 5389 ScaI AGT'ACT Cuts at: 3637 5349 3637 Size: 1712 3677 SfcI C'TryA_G Cuts at: 826 1045 2737 3872 4553 4744 826 Size: 219 1692 1135 681 191 1471 Fragments arranged by size: 1692 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2206 2206 Size: 5389 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5389 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5389 SmaI CCC'GGG Cuts at: 1872 2716 1872 Size: 844 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3493 4394 4656 820 Size: 197 926 1550 901 262 1553 Fragments arranged by size: 1553 1550 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3180 493 Size: 2687 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5389 SphI G_CATG'C Cuts at: 1132 2269 2747 1132 Size: 1137 478 3774 Fragments arranged by size: 3774 1137 478 SspI AAT'ATT Cuts at: 5 52 3313 5 Size: 47 3261 2081 Fragments arranged by size: 3261 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5389 StyI C'CwwG_G Cuts at: 513 1065 2406 2828 513 Size: 552 1341 422 3074 Fragments arranged by size: 3074 1341 552 422 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 3433 3586 3771 4216 325 Size: 1255 1836 17 153 185 445 1498 Fragments arranged by size: 1836 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3635 5347 96 Size: 274 80 33 51 1443 1658 1712 138 Fragments arranged by size: 1712 1658 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2246 2278 5349 5381 2246 Size: 32 3071 32 2254 Fragments arranged by size: 3071 2254 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5389 XbaI T'CTAG_A Cuts at: 2725 2725 Size: 5389 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5389 XmaI C'CCGG_G Cuts at: 1870 2714 1870 Size: 844 4545 XmnI GAAnn'nnTTC Cuts at: 3518 3518 Size: 5389 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3197 276 Size: 53 83 186 936 1663 2468 Fragments arranged by size: 2468 1663 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI