(Linear) (Six-base) MAPSORT of: KIEE1546.seq Check: 5333 from: 1 to: 684 KIEE1546.orf With 182 enzymes: SgfI * July 15, 2010 18:41 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 621 684 Size: 621 63 ApoI r'AATT_y Cuts at: 0 574 684 Size: 574 110 BanI G'GyrC_C Cuts at: 0 16 199 684 Size: 16 183 485 Fragments arranged by size: 485 183 16 BanII G_rGCy'C Cuts at: 0 413 684 Size: 413 271 BbeI G_GCGC'C Cuts at: 0 203 684 Size: 203 481 BbsI GAAGACnn'nnnn_ Cuts at: 0 555 684 Size: 555 129 BmrI ACTGGGnnnn_n' Cuts at: 0 249 684 Size: 249 435 BsaHI Gr'CG_yC Cuts at: 0 200 684 Size: 200 484 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 86 116 241 271 684 Size: 86 30 125 30 413 Fragments arranged by size: 413 125 86 30 30 BseYI C'CCAG_C Cuts at: 0 264 413 466 684 Size: 264 149 53 218 Fragments arranged by size: 264 218 149 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 684 Size: 265 419 Bsp1286I G_dGCh'C Cuts at: 0 413 684 Size: 413 271 BspHI T'CATG_A Cuts at: 0 206 617 684 Size: 206 411 67 Fragments arranged by size: 411 206 67 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 44 684 Size: 44 640 BsrDI GCAATG_nn' Cuts at: 0 605 684 Size: 605 79 BsrFI r'CCGG_y Cuts at: 0 3 196 684 Size: 3 193 488 Fragments arranged by size: 488 193 3 BstAPI GCAn_nnn'nTGC Cuts at: 0 23 684 Size: 23 661 BtgI C'CryG_G Cuts at: 0 611 684 Size: 611 73 BtsI GCAGTG_nn' Cuts at: 0 599 684 Size: 599 85 DraIII CAC_nnn'GTG Cuts at: 0 23 684 Size: 23 661 EaeI y'GGCC_r Cuts at: 0 317 684 Size: 317 367 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 621 684 Size: 621 63 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 684 Size: 147 537 HaeII r_GCGC'y Cuts at: 0 203 684 Size: 203 481 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 86 118 684 Size: 86 32 566 Fragments arranged by size: 566 86 32 KasI G'GCGC_C Cuts at: 0 199 684 Size: 199 485 MslI CAynn'nnrTG Cuts at: 0 341 610 684 Size: 341 269 74 Fragments arranged by size: 341 269 74 MspA1I CmG'CkG Cuts at: 0 417 684 Size: 417 267 NaeI GCC'GGC Cuts at: 0 198 684 Size: 198 486 NarI GG'CG_CC Cuts at: 0 200 684 Size: 200 484 NgoMIV G'CCGG_C Cuts at: 0 196 684 Size: 196 488 NspI r_CATG'y Cuts at: 0 264 684 Size: 264 420 PfoI T'CCnGG_A Cuts at: 0 362 684 Size: 362 322 SfoI GGC'GCC Cuts at: 0 201 684 Size: 201 483 SphI G_CATG'C Cuts at: 0 264 684 Size: 264 420 StyI C'CwwG_G Cuts at: 0 401 684 Size: 401 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 241 273 684 Size: 241 32 411 Fragments arranged by size: 411 241 32 Enzymes that do cut: AcuI ApoI BanI BanII BbeI BbsI BmrI BsaHI BsaXI BseYI BsgI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BtgI BtsI DraIII EaeI Eco57MI FalI HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI PfoI SfoI SphI StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI