################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MboII MfeI MflI MluI MroI MslI MunI NarI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 152 GAATTC [GA]^AATT[CT] AluI 249 AGCT AG^CT AlwNI 139 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 152 GAATTC [GA]^AATT[CT] AvaII 196 GGACC G^G[AT]CC BalI 110 TGGCCA TGG^CCA BsmFI 189 GTCCC GGGAC(10/14) BsrFI 86 GCCGGC [GA]^CCGG[CT] BsrI 129 CCAGT ACTGG(1/-1) BssHII 90 GCGCGC G^CGCGC BstAPI 138 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 72 CCTGG CC^[AT]GG Cfr10I 86 GCCGGC [GA]^CCGG[CT] DsaI 107 CCGTGG C^C[GA][CT]GG EcoRI 152 GAATTC G^AATTC EcoRII 72 CCTGG ^CC[AT]GG FauI 5 CCCGC CCCGC(4/6) HgaI 103 GCGTC GACGC(5/10) HindIII 248 AAGCTT A^AGCTT HphI 156 TCACC GGTGA(8/7) MaeII 182 ACGT A^CGT MaeIII 308 GTGAC ^GT[ACGT]AC MluNI 110 TGGCCA TGG^CCA MnlI 255 CCTC CCTC(7/6) MscI 110 TGGCCA TGG^CCA MseI 278 TTAA T^TAA MspA1I 80 CCGCTG C[AC]G^C[GT]G MvaI 72 CCTGG CC^[AT]GG NaeI 86 GCCGGC GCC^GGC NgoMIV 86 GCCGGC G^CCGGC PstI 145 CTGCAG CTGCA^G SbfI 144 CCTGCAGG CCTGCA^GG SfcI 145 CTGCAG C^T[GA][CT]AG SmlI 232 CTCAAG C^T[CT][GA]AG Sse8387I 144 CCTGCAGG CCTGCA^GG TaqI 290 TCGA T^CGA Tru9I 278 TTAA T^TAA Tsp45I 308 GTGAC ^GT[GC]AC Tsp509I 153 AATT ^AATT TthHB8I 290 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AccII 7 CGCG CG^CG 91 CGCG BcnI 38 CCCGG CC^[GC]GG 193 CCCGG BstF5I 168 CATCC GGATG(2/0) 266 CATCC BstUI 7 CGCG CG^CG 91 CGCG Cfr13I 3 GGCCC G^G[ACGT]CC 196 GGACC EaeI 84 TGGCCG [CT]^GGCC[GA] 110 TGGCCA FokI 168 CATCC GGATG(9/13) 266 CATCC MvnI 7 CGCG CG^CG 91 CGCG NciI 38 CCCGG CC^[GC]GG 193 CCCGG NlaIV 41 GGCTCC GG[ACGT]^[ACGT]CC 196 GGACCC Sau96I 3 GGCCC G^G[ACGT]CC 196 GGACC SfaNI 297 GATGC GCATC(5/9) 265 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BssKI Cac8I CfoI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MspI MwoI ScrFI TseI TspRI