(Circular) (Six-base) MAPSORT of: pFN21AB7983.seq Check: 8951 from: 1 to: 5971 pFN21AB7983 5971 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2134 2134 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2889 3314 1937 Size: 952 425 4594 Fragments arranged by size: 4594 952 425 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 2642 2775 4034 5418 5686 1632 Size: 968 42 133 1259 1384 268 1917 Fragments arranged by size: 1917 1384 1259 968 268 133 42 AfeI AGC'GCT Cuts at: 2986 2986 Size: 5971 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 2917 4802 2917 Size: 1885 4086 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2689 2721 2689 Size: 32 5939 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaI G_GGCC'C Cuts at: 2116 2116 Size: 5971 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2136 2169 3280 3506 3658 5767 1493 Size: 643 33 1111 226 152 2109 1697 Fragments arranged by size: 2109 1697 1111 643 226 152 33 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2721 3169 3296 1870 Size: 73 778 448 127 4545 Fragments arranged by size: 4545 778 448 127 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 2506 3164 3173 3292 Size: 325 217 627 687 32 658 9 119 Cuts at: 3292 4747 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 687 658 627 325 217 119 32 9 BanII G_rGCy'C Cuts at: 1391 2116 3290 1391 Size: 725 1174 4072 Fragments arranged by size: 4072 1174 725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2836 928 Size: 971 937 4063 Fragments arranged by size: 4063 971 937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4162 4196 1903 Size: 34 50 34 2141 34 3678 Fragments arranged by size: 3678 2141 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2502 3863 5073 2502 Size: 1361 1210 3400 Fragments arranged by size: 3400 1361 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2746 5966 2746 Size: 3220 2751 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2116 2288 2477 3167 3176 4032 5188 1790 Size: 326 172 189 690 9 856 1156 2573 Fragments arranged by size: 2573 1156 856 690 326 189 172 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 3035 3702 Size: 416 53 83 399 24 846 772 667 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 958 846 772 667 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2102 2400 1056 Size: 1046 298 4627 Fragments arranged by size: 4627 1046 298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2212 2212 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 2699 4631 1239 Size: 990 470 1932 2579 Fragments arranged by size: 2579 1932 990 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3762 493 Size: 711 2558 2702 Fragments arranged by size: 2702 2558 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2462 2917 4404 4802 5076 5223 5634 2462 Size: 455 1487 398 274 147 411 2799 Fragments arranged by size: 2799 1487 455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2273 2303 2672 2702 1178 Size: 30 165 30 870 30 369 30 4447 Fragments arranged by size: 4447 870 369 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3049 1186 Size: 1863 4108 BseYI C'CCAG_C Cuts at: 2469 3002 3047 5174 5670 2469 Size: 533 45 2127 496 2770 Fragments arranged by size: 2770 2127 533 496 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5398 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2120 3421 4182 5210 1540 Size: 275 186 119 1301 761 1028 2301 Fragments arranged by size: 2301 1301 1028 761 275 186 119 BsiHKAI G_wGCw'C Cuts at: 4032 4117 5188 4032 Size: 85 1071 4815 Fragments arranged by size: 4815 1071 85 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2116 2288 2477 3167 3176 3290 4032 Size: 399 326 172 189 690 9 114 742 Cuts at: 4032 4117 5188 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 690 399 326 189 172 114 85 9 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2134 2169 3033 3326 844 Size: 1290 35 864 293 3489 Fragments arranged by size: 3489 1290 864 293 35 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2917 4615 4802 1700 Size: 110 1107 1698 187 2869 Fragments arranged by size: 2869 1698 1107 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2746 3301 3668 4053 4070 5522 5608 5620 Size: 774 555 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 774 555 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3012 513 Size: 552 1947 3472 Fragments arranged by size: 3472 1947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 2063 3487 4281 4301 2063 Size: 1424 794 20 3733 Fragments arranged by size: 3733 1424 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2060 2117 3257 Size: 54 1425 50 275 18 230 57 1140 Cuts at: 3257 3418 4307 5936 8 Size: 161 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 275 230 161 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2117 3418 1537 Size: 275 305 1301 4090 Fragments arranged by size: 4090 1301 305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2797 3901 1353 Size: 1444 1104 3423 Fragments arranged by size: 3423 1444 1104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3118 4558 4932 5078 387 Size: 1064 138 1529 1440 374 146 1280 Fragments arranged by size: 1529 1440 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2600 2642 2699 2775 4034 4631 Size: 393 597 371 42 57 76 1259 597 Cuts at: 4631 5418 5686 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1259 787 597 597 393 371 268 76 57 42 EcoO109I rG'GnC_Cy Cuts at: 2112 2674 2112 Size: 562 5409 EcoRI G'AATT_C Cuts at: 2136 3280 3658 2136 Size: 1144 378 4449 Fragments arranged by size: 4449 1144 378 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2988 5118 1678 Size: 1310 2130 2531 Fragments arranged by size: 2531 2130 1310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2273 2305 4612 4644 4686 Size: 32 135 32 701 32 2307 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 2307 701 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2053 3315 3561 669 Size: 988 281 115 1262 246 3079 Fragments arranged by size: 3079 1262 988 281 246 115 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2175 2604 5085 5269 1305 Size: 870 429 2481 184 2007 Fragments arranged by size: 2481 2007 870 429 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3259 10 Size: 54 1425 343 1427 2722 Fragments arranged by size: 2722 1427 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3929 4288 4447 518 Size: 648 2763 359 159 2042 Fragments arranged by size: 2763 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2109 2620 3387 4064 5212 5457 1606 Size: 416 87 511 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 511 416 245 87 NcoI C'CATG_G Cuts at: 513 1065 3012 513 Size: 552 1947 3472 Fragments arranged by size: 3472 1947 552 NdeI CA'TA_TG Cuts at: 387 3132 387 Size: 2745 3226 NheI G'CTAG_C Cuts at: 1052 2098 2396 1052 Size: 1046 298 4627 Fragments arranged by size: 4627 1046 298 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2431 3076 3329 4874 1132 Size: 97 1202 645 253 1545 2229 Fragments arranged by size: 2229 1545 1202 645 253 97 PasI CC'CwG_GG Cuts at: 1348 2208 1348 Size: 860 5111 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PfoI T'CCnGG_A Cuts at: 3226 3226 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PspOMI G'GGCC_C Cuts at: 2112 2112 Size: 5971 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2638 2670 2638 Size: 32 5939 PstI C_TGCA'G Cuts at: 830 2145 3323 830 Size: 1315 1178 3478 Fragments arranged by size: 3478 1315 1178 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2620 2620 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2141 2980 3319 4454 5135 5326 826 Size: 219 1096 839 339 1135 681 191 1471 Fragments arranged by size: 1471 1135 1096 839 681 339 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3076 3329 1132 Size: 1944 253 3774 Fragments arranged by size: 3774 1944 253 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 3012 3410 513 Size: 552 1947 398 3074 Fragments arranged by size: 3074 1947 552 398 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3010 3010 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI