(Circular) (Six-base) MAPSORT of: pFN21AE2221.seq Check: 4757 from: 1 to: 5710 pFN21AE2221 5710 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3520 278 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2675 3053 1937 Size: 738 378 4594 Fragments arranged by size: 4594 738 378 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2314 3773 5157 5425 1632 Size: 492 190 1459 1384 268 1917 Fragments arranged by size: 1917 1459 1384 492 268 190 AfeI AGC'GCT Cuts at: 2391 2391 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5513 AflIII A'CryG_T Cuts at: 1203 2706 4609 5586 1203 Size: 1503 1903 977 1327 Fragments arranged by size: 1903 1503 1327 977 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 2559 4439 2559 Size: 1880 3830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5710 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 3767 4923 3767 Size: 1156 4554 ApoI r'AATT_y Cuts at: 1493 3019 3245 3397 5506 1493 Size: 1526 226 152 2109 1697 Fragments arranged by size: 2109 1697 1526 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2646 3035 1870 Size: 73 703 389 4545 Fragments arranged by size: 4545 703 389 73 BamHI G'GATC_C Cuts at: 2718 2854 2962 3040 3407 2718 Size: 136 108 78 367 5021 Fragments arranged by size: 5021 367 136 108 78 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 4486 618 Size: 325 217 627 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 325 217 BanII G_rGCy'C Cuts at: 1391 2412 2809 3029 1391 Size: 1021 397 220 4072 Fragments arranged by size: 4072 1021 397 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2130 928 Size: 971 231 4508 Fragments arranged by size: 4508 971 231 BbvCI CC'TCA_GC Cuts at: 2106 2106 Size: 5710 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 1548 Size: 5710 BfrBI ATG'CAT Cuts at: 2984 2984 Size: 5710 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 3127 3127 Size: 5710 Bme1580I G_kGCm'C Cuts at: 1790 2073 3771 4927 1790 Size: 283 1698 1156 2573 Fragments arranged by size: 2573 1698 1156 283 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2654 3441 4399 Size: 416 53 83 399 24 1237 787 958 Cuts at: 4399 442 Size: 1753 Fragments arranged by size: 1753 1237 958 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2039 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2396 4370 1239 Size: 1157 1974 2579 Fragments arranged by size: 2579 1974 1157 Bpu10I CC'TnA_GC Cuts at: 2106 2463 2106 Size: 357 5353 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 3835 4700 4998 2305 Size: 1530 865 298 3017 Fragments arranged by size: 3017 1530 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2338 2657 4373 882 Size: 617 839 319 1716 2219 Fragments arranged by size: 2219 1716 839 617 319 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3517 3899 4534 275 Size: 53 83 186 936 1984 382 635 1451 Fragments arranged by size: 1984 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4143 4541 4815 4962 5373 4143 Size: 398 274 147 411 4480 Fragments arranged by size: 4480 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2442 2472 2556 2586 1178 Size: 30 165 30 1039 30 84 30 4302 Fragments arranged by size: 4302 1039 165 84 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5710 BseYI C'CCAG_C Cuts at: 2796 4913 5409 2796 Size: 2117 496 3097 Fragments arranged by size: 3097 2117 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 1163 Size: 573 339 4798 Fragments arranged by size: 4798 573 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2809 3771 3856 4927 2809 Size: 962 85 1071 3592 Fragments arranged by size: 3592 1071 962 85 BsmI GAATG_Cn' Cuts at: 2986 3221 3314 2986 Size: 235 93 5382 Fragments arranged by size: 5382 235 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2412 2809 3029 3771 3856 4927 Size: 399 283 339 397 220 742 85 1071 Cuts at: 4927 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 397 339 283 220 85 BspEI T'CCGG_A Cuts at: 5373 5373 Size: 5710 BspHI T'CATG_A Cuts at: 2440 3599 5329 2440 Size: 1159 1730 2821 Fragments arranged by size: 2821 1730 1159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 3065 844 Size: 1253 968 3489 Fragments arranged by size: 3489 1253 968 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 2889 96 Size: 2793 2917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstXI CCAn_nnnn'nTGG Cuts at: 2383 2383 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2718 2854 2962 3040 3407 3792 3809 5261 Size: 746 136 108 78 367 385 17 1452 Cuts at: 5261 5347 5359 5705 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 746 385 367 346 136 108 86 78 17 12 BstZ17I GTA'TAC Cuts at: 2676 2676 Size: 5710 BtgI C'CryG_G Cuts at: 513 1065 2004 2906 2967 513 Size: 552 939 902 61 3256 Fragments arranged by size: 3256 939 902 552 61 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 2494 1554 Size: 940 4770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 3014 3014 Size: 5710 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 3157 4046 Size: 54 1425 50 275 18 234 1093 889 Cuts at: 4046 5675 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1093 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3640 1353 Size: 2287 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2807 2807 Size: 5710 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2314 2396 3773 4370 5157 5425 Size: 393 492 190 82 1377 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 1377 787 597 492 393 268 190 82 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2393 2797 4857 1678 Size: 715 404 2060 2531 Fragments arranged by size: 2531 2060 715 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 2278 2310 2335 Size: 32 135 32 585 32 89 32 25 Cuts at: 2335 2367 2509 2541 2545 2577 4351 4383 4425 Size: 32 142 32 4 32 1774 32 42 Cuts at: 4425 4457 1373 Size: 32 2626 Fragments arranged by size: 2626 1774 585 142 135 89 42 32 32 32 32 32 32 32 32 32 25 4 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5710 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 5586 5586 Size: 5710 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2182 2264 4824 5008 1305 Size: 877 82 2560 184 2007 Fragments arranged by size: 2560 2007 877 184 82 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3888 Fragments arranged by size: 3888 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 3668 4027 4186 518 Size: 648 1279 1223 359 159 2042 Fragments arranged by size: 2042 1279 1223 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2908 3126 3803 4951 5196 1606 Size: 1302 218 677 1148 245 2120 Fragments arranged by size: 2120 1302 1148 677 245 218 NcoI C'CATG_G Cuts at: 513 1065 2004 2967 513 Size: 552 939 963 3256 Fragments arranged by size: 3256 963 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NsiI A_TGCA'T Cuts at: 2986 2986 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2710 3068 4613 1132 Size: 97 1481 358 1545 2229 Fragments arranged by size: 2229 1545 1481 358 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5710 PciI A'CATG_T Cuts at: 2706 4609 2706 Size: 1903 3807 PflMI CCAn_nnn'nTGG Cuts at: 1108 2666 1108 Size: 1558 4152 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PstI C_TGCA'G Cuts at: 830 3062 830 Size: 2232 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 SacI G_AGCT'C Cuts at: 2809 2809 Size: 5710 SacII CC_GC'GG Cuts at: 2909 2909 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2476 3058 4193 4874 5065 826 Size: 219 1431 582 1135 681 191 1471 Fragments arranged by size: 1471 1431 1135 681 582 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 2646 3814 4715 4977 820 Size: 197 926 341 362 1168 901 262 1553 Fragments arranged by size: 1553 1168 926 901 362 341 262 197 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 3068 1132 Size: 1936 3774 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2967 3149 513 Size: 552 939 963 182 3074 Fragments arranged by size: 3074 963 939 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2183 2623 2788 3737 3754 3907 4092 Size: 1255 603 440 165 949 17 153 185 Cuts at: 4092 4537 325 Size: 445 1498 Fragments arranged by size: 1498 1255 949 603 445 440 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 2889 3956 Size: 274 80 33 51 1443 786 126 1067 Cuts at: 3956 5668 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1067 786 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2556 2588 5670 5702 2556 Size: 32 3082 32 2564 Fragments arranged by size: 3082 2564 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5710 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 2646 1943 Size: 703 5007 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 2783 3839 2783 Size: 1056 4654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3518 276 Size: 53 83 186 936 1984 2468 Fragments arranged by size: 2468 1984 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI