(Linear) (Six-base) MAPSORT of: KIEE2221.seq Check: 1413 from: 1 to: 1005 KIEE2221.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. AccI GT'mk_AC Cuts at: 0 670 1005 Size: 670 335 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 309 1005 Size: 119 190 696 Fragments arranged by size: 696 190 119 AfeI AGC'GCT Cuts at: 0 386 1005 Size: 386 619 AflIII A'CryG_T Cuts at: 0 701 1005 Size: 701 304 AhdI GACnn_n'nnGTC Cuts at: 0 554 1005 Size: 554 451 AvaI C'yCGr_G Cuts at: 0 641 1005 Size: 641 364 BamHI G'GATC_C Cuts at: 0 713 849 957 1005 Size: 713 136 108 48 Fragments arranged by size: 713 136 108 48 BanII G_rGCy'C Cuts at: 0 407 804 1005 Size: 407 397 201 Fragments arranged by size: 407 397 201 BbsI GAAGACnn'nnnn_ Cuts at: 0 125 1005 Size: 125 880 BbvCI CC'TCA_GC Cuts at: 0 101 1005 Size: 101 904 BfrBI ATG'CAT Cuts at: 0 979 1005 Size: 979 26 Bme1580I G_kGCm'C Cuts at: 0 68 1005 Size: 68 937 BmrI ACTGGGnnnn_n' Cuts at: 0 649 1005 Size: 649 356 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 34 1005 Size: 34 971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 1005 Size: 391 614 Bpu10I CC'TnA_GC Cuts at: 0 101 458 1005 Size: 101 357 547 Fragments arranged by size: 547 357 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 1005 Size: 300 705 BsaI GGTCTCn'nnnn_ Cuts at: 0 333 652 1005 Size: 333 319 353 Fragments arranged by size: 353 333 319 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 437 467 551 581 1005 Size: 437 30 84 30 424 Fragments arranged by size: 437 424 84 30 30 BseYI C'CCAG_C Cuts at: 0 791 1005 Size: 791 214 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 1005 Size: 70 935 BsiHKAI G_wGCw'C Cuts at: 0 804 1005 Size: 804 201 BsmI GAATG_Cn' Cuts at: 0 981 1005 Size: 981 24 Bsp1286I G_dGCh'C Cuts at: 0 68 407 804 1005 Size: 68 339 397 201 Fragments arranged by size: 397 339 201 68 BspHI T'CATG_A Cuts at: 0 435 1005 Size: 435 570 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 92 1005 Size: 92 913 BsrGI T'GTAC_A Cuts at: 0 884 1005 Size: 884 121 BstXI CCAn_nnnn'nTGG Cuts at: 0 378 1005 Size: 378 627 BstYI r'GATC_y Cuts at: 0 713 849 957 1005 Size: 713 136 108 48 Fragments arranged by size: 713 136 108 48 BstZ17I GTA'TAC Cuts at: 0 671 1005 Size: 671 334 BtgI C'CryG_G Cuts at: 0 901 962 1005 Size: 901 61 43 Fragments arranged by size: 901 61 43 ClaI AT'CG_AT Cuts at: 0 489 1005 Size: 489 516 EaeI y'GGCC_r Cuts at: 0 59 1005 Size: 59 946 EcoICRI GAG'CTC Cuts at: 0 802 1005 Size: 802 203 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 119 309 391 1005 Size: 119 190 82 614 Fragments arranged by size: 614 190 119 82 HaeII r_GCGC'y Cuts at: 0 388 792 1005 Size: 388 404 213 Fragments arranged by size: 404 388 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 152 184 273 305 330 362 504 536 Size: 152 32 89 32 25 32 142 32 Cuts at: 536 540 572 1005 Size: 4 32 433 Fragments arranged by size: 433 152 142 89 32 32 32 32 32 25 4 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 177 259 1005 Size: 177 82 746 Fragments arranged by size: 746 177 82 MslI CAynn'nnrTG Cuts at: 0 440 1005 Size: 440 565 MspA1I CmG'CkG Cuts at: 0 903 1005 Size: 903 102 NcoI C'CATG_G Cuts at: 0 962 1005 Size: 962 43 NsiI A_TGCA'T Cuts at: 0 981 1005 Size: 981 24 NspI r_CATG'y Cuts at: 0 705 1005 Size: 705 300 PciI A'CATG_T Cuts at: 0 701 1005 Size: 701 304 PflMI CCAn_nnn'nTGG Cuts at: 0 661 1005 Size: 661 344 SacI G_AGCT'C Cuts at: 0 804 1005 Size: 804 201 SacII CC_GC'GG Cuts at: 0 904 1005 Size: 904 101 SfcI C'TryA_G Cuts at: 0 471 1005 Size: 471 534 SmlI C'TyrA_G Cuts at: 0 279 641 1005 Size: 279 362 364 Fragments arranged by size: 364 362 279 StyI C'CwwG_G Cuts at: 0 962 1005 Size: 962 43 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 178 618 783 1005 Size: 178 440 165 222 Fragments arranged by size: 440 222 178 165 TatI w'GTAC_w Cuts at: 0 758 884 1005 Size: 758 126 121 Fragments arranged by size: 758 126 121 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 551 583 1005 Size: 551 32 422 Fragments arranged by size: 551 422 32 XhoI C'TCGA_G Cuts at: 0 641 1005 Size: 641 364 XmnI GAAnn'nnTTC Cuts at: 0 778 1005 Size: 778 227 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AvaI BamHI BanII BbsI BbvCI BfrBI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstXI BstYI BstZ17I BtgI ClaI EaeI EcoICRI Eco57MI HaeII Hin4I MmeI MslI MspA1I NcoI NsiI NspI PciI PflMI SacI SacII SfcI SmlI StyI TaqII TatI TstI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XmaI ZraI