################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI Ksp632I KspI MamI MboI MflI MluI MroI MslI MspI MvnI NaeI NarI NciI NcoI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI Sau3AI Sau96I SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1180 GTAGAC GT^[AC][GT]AC AflIII 771 ACACGT A^C[GA][CT]GT Alw44I 1035 GTGCAC G^TGCAC ApaLI 1035 GTGCAC G^TGCAC BalI 683 TGGCCA TGG^CCA BanII 1004 GAGCTC G[GA]GC[CT]^C BfaI 131 CTAG C^TAG BsaAI 1112 TACGTG [CT]AC^GT[GA] BsaJI 369 CCTGGG C^C[ACGT][ACGT]GG BseRI 1052 CTCCTC GAGGAG(10/8) BsiYI 369 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 369 CCTGGGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsrDI 852 CATTGC GCAATG(2/0) BstAPI 847 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 1117 GCTGGC GC[ACGT]^[ACGT]GC DraIII 587 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 683 TGGCCA [CT]^GGCC[GA] HaeIII 684 GGCC GG^CC HincII 468 GTTGAC GT[CT]^[GA]AC HindII 468 GTTGAC GT[CT]^[GA]AC HindIII 1205 AAGCTT A^AGCTT HinfI 308 GAGTC G^A[ACGT]TC HphI 592 GGTGA GGTGA(8/7) MaeI 131 CTAG C^TAG MfeI 332 CAATTG C^AATTG MluNI 683 TGGCCA TGG^CCA MscI 683 TGGCCA TGG^CCA MunI 332 CAATTG C^AATTG NdeI 439 CATATG CA^TATG PleI 308 GAGTC GAGTC(4/5) PstI 20 CTGCAG CTGCA^G SacI 1004 GAGCTC GAGCT^C ScaI 957 AGTACT AGT^ACT SexAI 588 ACCAGGT A^CC[AT]GGT SfcI 20 CTGCAG C^T[GA][CT]AG SpeI 130 ACTAGT A^CTAGT SspI 268 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 999 CCGC CCGC(-3/-1) 1152 CCGC AlwNI 146 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 149 CAGCTGCTG Asp700I 265 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 617 GAAGACTTTC BbsI 617 GAAGAC GAAGAC(2/6) 837 GTCTTC BpmI 239 CTCCAG CTGGAG(16/14) 512 CTGGAG BpuAI 617 GAAGAC GAAGAC(2/6) 837 GTCTTC BsmAI 932 GAGAC GTCTC(1/5) 310 GTCTC DdeI 14 CTAAG C^T[ACGT]AG 327 CTCAG DraI 34 TTTAAA TTT^AAA 216 TTTAAA MaeII 773 ACGT A^CGT 1113 ACGT MspA1I 149 CAGCTG C[AC]G^C[GT]G 397 CAGCTG MwoI 292 GCTCTTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 847 GCAGACATTGC NspI 596 ACATGC [GA]CATG^[CT] 880 GCATGT PvuII 149 CAGCTG CAG^CTG 397 CAGCTG XcmI 431 CCATTCATCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 671 CCATAGAACATTTGG XmnI 265 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 617 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI BbvI BmyI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI EcoRII Fnu4HI FokI ItaI MaeIII MboII MnlI MseI MvaI NlaIII RsaI ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI