(Circular) (Six-base) MAPSORT of: pFN21AE5507.seq Check: 1980 from: 1 to: 5947 pFN21AE5507 5947 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3186 3290 1937 Size: 1249 104 4594 Fragments arranged by size: 4594 1249 104 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 2829 4010 5394 5662 1632 Size: 600 597 1181 1384 268 1917 Fragments arranged by size: 1917 1384 1181 600 597 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2776 4846 5823 1203 Size: 1573 2070 977 1327 Fragments arranged by size: 2070 1573 1327 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5947 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3055 1184 Size: 1871 4076 AlwNI CAG_nnn'CTG Cuts at: 2156 2159 3690 5262 2156 Size: 3 1531 1572 2841 Fragments arranged by size: 2841 1572 1531 3 ApaLI G'TGCA_C Cuts at: 3040 4004 5160 3040 Size: 964 1156 3827 Fragments arranged by size: 3827 1156 964 ApoI r'AATT_y Cuts at: 1493 2066 2489 3018 3256 3482 3634 5743 1493 Size: 573 423 529 238 226 152 2109 1697 Fragments arranged by size: 2109 1697 573 529 423 238 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 4723 618 Size: 325 217 627 1481 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3013 3266 1391 Size: 1622 253 4072 Fragments arranged by size: 4072 1622 253 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2629 2835 928 Size: 971 730 206 4040 Fragments arranged by size: 4040 971 730 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4138 4172 1903 Size: 34 50 34 2117 34 3678 Fragments arranged by size: 3678 2117 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4558 136 Size: 107 122 71 631 605 2886 1525 Fragments arranged by size: 2886 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 3044 4008 5164 1790 Size: 1254 964 1156 2573 Fragments arranged by size: 2573 1254 1156 964 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3678 4636 442 Size: 416 53 83 399 24 2261 958 1753 Fragments arranged by size: 2261 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 2538 4607 1239 Size: 990 309 2069 2579 Fragments arranged by size: 2579 2069 990 309 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4072 4937 5235 4072 Size: 865 298 4784 Fragments arranged by size: 4784 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3119 3738 493 Size: 711 1915 619 2702 Fragments arranged by size: 2702 1915 711 619 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5722 Fragments arranged by size: 5722 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3048 1186 Size: 1862 4085 BseYI C'CCAG_C Cuts at: 5150 5646 5150 Size: 496 5451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2654 3013 3044 4008 4093 5164 2654 Size: 359 31 964 85 1071 3437 Fragments arranged by size: 3437 1071 964 359 85 31 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2654 3013 3044 3266 4008 4093 5164 Size: 399 864 359 31 222 742 85 1071 Cuts at: 5164 1391 Size: 2174 Fragments arranged by size: 2174 1071 864 742 399 359 222 85 31 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3302 844 Size: 2458 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2856 4442 4616 65 Size: 1183 1608 1586 174 1396 Fragments arranged by size: 1608 1586 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 4778 1700 Size: 110 2781 187 2869 Fragments arranged by size: 2869 2781 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 2858 794 Size: 2064 3883 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3277 3644 4029 4046 5498 5584 5596 5942 Size: 1305 367 385 17 1452 86 12 346 Cuts at: 5942 1972 Size: 1977 Fragments arranged by size: 1977 1452 1305 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5395 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 3463 4257 4277 3463 Size: 794 20 5133 Fragments arranged by size: 5133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 2041 2223 3251 2041 Size: 182 1028 4737 Fragments arranged by size: 4737 1028 182 DraIII CAC_nnn'GTG Cuts at: 2597 2597 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2688 3394 4283 Size: 54 1425 50 275 18 858 706 889 Cuts at: 4283 5912 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 858 706 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3877 1353 Size: 2524 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3011 3011 Size: 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2232 2538 2829 4010 4607 5394 Size: 393 597 3 306 291 1181 597 787 Cuts at: 5394 5662 1239 Size: 268 1524 Fragments arranged by size: 1524 1181 787 597 597 393 306 291 268 3 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2315 2646 2315 Size: 331 5616 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 5094 1678 Size: 3416 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 4588 4620 4662 Size: 32 135 32 655 32 2329 32 42 Cuts at: 4662 4694 1373 Size: 32 2626 Fragments arranged by size: 2626 2329 655 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2475 3291 3537 669 Size: 988 281 537 816 246 3079 Fragments arranged by size: 3079 988 816 537 281 246 HindIII A'AGCT_T Cuts at: 748 3210 748 Size: 2462 3485 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 2337 3546 2337 Size: 1209 4738 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3184 5061 5245 1305 Size: 1879 1877 184 2007 Fragments arranged by size: 2007 1879 1877 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 10 Size: 54 1425 343 858 3267 Fragments arranged by size: 3267 1425 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3905 4264 4423 518 Size: 648 2739 359 159 2042 Fragments arranged by size: 2739 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2156 2404 3363 4040 5188 5433 1606 Size: 550 248 959 677 1148 245 2120 Fragments arranged by size: 2120 1148 959 677 550 248 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5395 NdeI CA'TA_TG Cuts at: 387 2445 387 Size: 2058 3889 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2605 2889 3305 4850 1132 Size: 97 1376 284 416 1545 2229 Fragments arranged by size: 2229 1545 1376 416 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5947 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5947 PfoI T'CCnGG_A Cuts at: 2092 2092 Size: 5947 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PstI C_TGCA'G Cuts at: 830 2029 3299 830 Size: 1199 1270 3478 Fragments arranged by size: 3478 1270 1199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 PvuII CAG'CTG Cuts at: 2156 2404 2156 Size: 248 5699 SacI G_AGCT'C Cuts at: 3013 3013 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 2964 4195 5907 2964 Size: 1231 1712 3004 Fragments arranged by size: 3004 1712 1231 SexAI A'CCwGG_T Cuts at: 2593 2593 Size: 5947 SfcI C'TryA_G Cuts at: 826 1045 2025 3295 4430 5111 5302 826 Size: 219 980 1270 1135 681 191 1471 Fragments arranged by size: 1471 1270 1135 980 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4051 4952 5214 820 Size: 197 926 2108 901 262 1553 Fragments arranged by size: 2108 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 2135 152 Size: 1983 3964 SphI G_CATG'C Cuts at: 1132 3305 1132 Size: 2173 3774 SspI AAT'ATT Cuts at: 5 52 2275 3871 5 Size: 47 2223 1596 2081 Fragments arranged by size: 2223 2081 1596 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5947 StyI C'CwwG_G Cuts at: 513 1065 3386 513 Size: 552 2321 3074 Fragments arranged by size: 3074 2321 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3974 3991 4144 4329 4774 325 Size: 1255 2394 17 153 185 445 1498 Fragments arranged by size: 2394 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2287 2869 2962 Size: 274 80 33 51 1443 310 582 93 Cuts at: 2962 4193 5905 96 Size: 1231 1712 138 Fragments arranged by size: 1712 1443 1231 582 310 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5907 5939 5907 Size: 32 5915 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2683 2443 Size: 240 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 2274 2626 4076 2274 Size: 352 1450 4145 Fragments arranged by size: 4145 1450 352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI