(Linear) (Six-base) MAPSORT of: KIEE5507.seq Check: 8318 from: 1 to: 1242 KIEE5507.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AccI GT'mk_AC Cuts at: 0 1181 1242 Size: 1181 61 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 227 824 1242 Size: 227 597 418 Fragments arranged by size: 597 418 227 AflIII A'CryG_T Cuts at: 0 771 1242 Size: 771 471 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1050 1242 Size: 1050 192 AlwNI CAG_nnn'CTG Cuts at: 0 151 154 1242 Size: 151 3 1088 Fragments arranged by size: 1088 151 3 ApaLI G'TGCA_C Cuts at: 0 1035 1242 Size: 1035 207 ApoI r'AATT_y Cuts at: 0 61 484 1013 1242 Size: 61 423 529 229 Fragments arranged by size: 529 423 229 61 BanII G_rGCy'C Cuts at: 0 1008 1242 Size: 1008 234 BbsI GAAGACnn'nnnn_ Cuts at: 0 624 830 1242 Size: 624 206 412 Fragments arranged by size: 624 412 206 Bme1580I G_kGCm'C Cuts at: 0 1039 1242 Size: 1039 203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 533 1242 Size: 224 309 709 Fragments arranged by size: 709 309 224 BsaAI yAC'GTr Cuts at: 0 1114 1242 Size: 1114 128 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1043 1242 Size: 1043 199 BsiHKAI G_wGCw'C Cuts at: 0 649 1008 1039 1242 Size: 649 359 31 203 Fragments arranged by size: 649 359 203 31 Bsp1286I G_dGCh'C Cuts at: 0 649 1008 1039 1242 Size: 649 359 31 203 Fragments arranged by size: 649 359 203 31 BsrDI GCAATG_nn' Cuts at: 0 851 1242 Size: 851 391 BstAPI GCAn_nnn'nTGC Cuts at: 0 853 1242 Size: 853 389 DraI TTT'AAA Cuts at: 0 36 218 1242 Size: 36 182 1024 Fragments arranged by size: 1024 182 36 DraIII CAC_nnn'GTG Cuts at: 0 592 1242 Size: 592 650 EaeI y'GGCC_r Cuts at: 0 683 1242 Size: 683 559 EcoICRI GAG'CTC Cuts at: 0 1006 1242 Size: 1006 236 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 227 533 824 1242 Size: 224 3 306 291 418 Fragments arranged by size: 418 306 291 224 3 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 310 641 1242 Size: 310 331 601 Fragments arranged by size: 601 331 310 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 222 254 1242 Size: 222 32 988 Fragments arranged by size: 988 222 32 HincII GTy'rAC Cuts at: 0 470 1242 Size: 470 772 HindIII A'AGCT_T Cuts at: 0 1205 1242 Size: 1205 37 MfeI C'AATT_G Cuts at: 0 332 1242 Size: 332 910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1179 1242 Size: 1179 63 MscI TGG'CCA Cuts at: 0 685 1242 Size: 685 557 MspA1I CmG'CkG Cuts at: 0 151 399 1242 Size: 151 248 843 Fragments arranged by size: 843 248 151 NdeI CA'TA_TG Cuts at: 0 440 1242 Size: 440 802 NspI r_CATG'y Cuts at: 0 600 884 1242 Size: 600 284 358 Fragments arranged by size: 600 358 284 PfoI T'CCnGG_A Cuts at: 0 87 1242 Size: 87 1155 PstI C_TGCA'G Cuts at: 0 24 1242 Size: 24 1218 PvuII CAG'CTG Cuts at: 0 151 399 1242 Size: 151 248 843 Fragments arranged by size: 843 248 151 SacI G_AGCT'C Cuts at: 0 1008 1242 Size: 1008 234 ScaI AGT'ACT Cuts at: 0 959 1242 Size: 959 283 SexAI A'CCwGG_T Cuts at: 0 588 1242 Size: 588 654 SfcI C'TryA_G Cuts at: 0 20 1242 Size: 20 1222 SpeI A'CTAG_T Cuts at: 0 130 1242 Size: 130 1112 SspI AAT'ATT Cuts at: 0 270 1242 Size: 270 972 TatI w'GTAC_w Cuts at: 0 282 864 957 1242 Size: 282 582 93 285 Fragments arranged by size: 582 285 282 93 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 438 678 1242 Size: 438 240 564 Fragments arranged by size: 564 438 240 XmnI GAAnn'nnTTC Cuts at: 0 269 621 1242 Size: 269 352 621 Fragments arranged by size: 621 352 269 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaLI ApoI BanII BbsI Bme1580I BpmI BsaAI BseRI BsiHKAI Bsp1286I BsrDI BstAPI DraI DraIII EaeI EcoICRI Eco57MI FalI Hin4I HincII HindIII MfeI MmeI MscI MspA1I NdeI NspI PfoI PstI PvuII SacI ScaI SexAI SfcI SpeI SspI TatI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EagI EarI EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XhoI XmaI ZraI