################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HapII Hin1I HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Asp700I 238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 332 CCCGAG C^[CT]CG[GA]G AvrII 757 CCTAGG C^CTAGG BanII 833 GAGCCC G[GA]GC[CT]^C BbsI 1021 GAAGAC GAAGAC(2/6) BciVI 1093 GTATCC GTATCC(6/5) BlnI 757 CCTAGG C^CTAGG BpuAI 1021 GAAGAC GAAGAC(2/6) BsaI 307 GGTCTC GGTCTC(1/5) BsgI 61 GTGCAG GTGCAG(16/14) BsoBI 332 CCCGAG C^[CT]CG[GA]G Bsp1407I 459 TGTACA T^GTACA BspMI 53 ACCTGC ACCTGC(4/8) BsrGI 459 TGTACA T^GTACA BssKI 825 CCTGG ^CC[ACGT]GG BstF5I 792 GGATG GGATG(2/0) BstNI 825 CCTGG CC^[AT]GG CfoI 399 GCGC GCG^C EagI 14 CGGCCG C^GGCCG EarI 1009 GAAGAG CTCTTC(1/4) EclXI 14 CGGCCG C^GGCCG Eco52I 14 CGGCCG C^GGCCG EcoRII 825 CCTGG ^CC[AT]GG EcoT14I 757 CCTAGG C^C[AT][AT]GG FokI 792 GGATG GGATG(9/13) HaeII 398 GGCGCT [GA]GCGC^[CT] HgaI 66 GACGC GACGC(5/10) HhaI 399 GCGC GCG^C HinP1I 399 GCGC G^CGC HincII 404 GTCAAC GT[CT]^[GA]AC HindII 404 GTCAAC GT[CT]^[GA]AC HindIII 1063 AAGCTT A^AGCTT Ksp632I 1009 GAAGAG CTCTTC(1/4) MaeIII 477 GTCAC ^GT[ACGT]AC MvaI 825 CCTGG CC^[AT]GG NlaIII 604 CATG CATG^ NotI 13 GCGGCCGC GC^GGCCGC PflMI 424 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 116 CAGCTG CAG^CTG ScrFI 825 CCTGG CC^[ACGT]GG SspBI 459 TGTACA T^GTACA StyI 757 CCTAGG C^C[AT][AT]GG TfiI 697 GATTC G^A[AT]TC Tsp45I 477 GTCAC ^GT[GC]AC Van91I 424 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 238 GAATTT [GA]^AATT[CT] 324 GAATTT Alw44I 440 GTGCAC G^TGCAC 497 GTGCAC AlwNI 64 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG 396 CAGGCGCTG ApaLI 440 GTGCAC G^TGCAC 497 GTGCAC ApoI 238 GAATTT [GA]^AATT[CT] 324 GAATTT AspHI 440 GTGCAC G[AT]GC[AT]^C 497 GTGCAC BclI 392 TGATCA T^GATCA 896 TGATCA BglII 885 AGATCT A^GATCT 1083 AGATCT BpmI 70 CTGGAG CTGGAG(16/14) 932 CTGGAG BseRI 31 GAGGAG GAGGAG(10/8) 136 GAGGAG BsiEI 14 CGGCCG CG[GA][CT]^CG 210 CGGTCG BsiHKAI 440 GTGCAC G[AT]GC[AT]^C 497 GTGCAC BsmAI 308 GTCTC GTCTC(1/5) 556 GTCTC BsmFI 50 GGGAC GGGAC(10/14) 600 GTCCC Cac8I 661 GCTGGC GC[ACGT]^[ACGT]GC 765 GCAAGC DraI 195 TTTAAA TTT^AAA 843 TTTAAA DraII 50 GGGACCT [GA]G^G[ACGT]CC[CT] 83 GGGTCCT EaeI 14 CGGCCG [CT]^GGCC[GA] 26 TGGCCG EcoO109I 50 GGGACCT [GA]G^G[ACGT]CC[CT] 83 GGGTCCT FbaI 392 TGATCA T^GATCA 896 TGATCA HphI 1006 GGTGA GGTGA(8/7) 478 TCACC MspA1I 78 CAGCGG C[AC]G^C[GT]G 116 CAGCTG PleI 815 GACTC GAGTC(4/5) 850 GACTC PpuMI 50 GGGACCT [GA]G^G[AT]CC[CT] 83 GGGTCCT SfaNI 793 GATGC GCATC(5/9) 95 GCATC TaqI 311 TCGA T^CGA 318 TCGA TthHB8I 311 TCGA T^CGA 318 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BstYI Cfr13I DdeI DpnII FauI Fnu4HI HaeIII HinfI ItaI MaeI MboI MboII MflI MnlI MseI MwoI NdeII NlaIV RsaI Sau3AI Sau96I Tru9I TseI Tsp509I TspRI XhoII