(Circular) (Six-base) MAPSORT of: pFN21AE1347.seq Check: 1591 from: 1 to: 5842 pFN21AE1347 5842 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3652 278 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 3905 5289 5557 1632 Size: 602 1671 1384 268 1917 Fragments arranged by size: 1917 1671 1384 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 2074 2406 3585 5157 2074 Size: 332 1179 1572 2759 Fragments arranged by size: 2759 1572 1179 332 ApaLI G'TGCA_C Cuts at: 2445 2502 3899 5055 2445 Size: 57 1397 1156 3232 Fragments arranged by size: 3232 1397 1156 57 ApoI r'AATT_y Cuts at: 1493 2243 2329 3151 3377 3529 5638 1493 Size: 750 86 822 226 152 2109 1697 Fragments arranged by size: 2109 1697 822 750 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2337 3167 1870 Size: 73 394 830 4545 Fragments arranged by size: 4545 830 394 73 AvrII C'CTAG_G Cuts at: 2762 2762 Size: 5842 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2544 2577 2544 Size: 33 5809 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3163 4618 618 Size: 325 217 627 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 627 325 217 BanII G_rGCy'C Cuts at: 1391 2842 3161 1391 Size: 1451 319 4072 Fragments arranged by size: 4072 1451 319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3033 928 Size: 971 1134 3737 Fragments arranged by size: 3737 1134 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3109 3734 4944 3109 Size: 625 1210 4007 Fragments arranged by size: 4007 1210 625 BclI T'GATC_A Cuts at: 1548 2397 2901 1548 Size: 849 504 4489 Fragments arranged by size: 4489 849 504 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4453 136 Size: 107 122 71 631 605 337 2444 1525 Fragments arranged by size: 2444 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2890 3088 5837 2890 Size: 198 2749 2895 Fragments arranged by size: 2895 2749 198 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2449 2506 3903 5059 1790 Size: 659 57 1397 1156 2573 Fragments arranged by size: 2573 1397 1156 659 57 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2445 3573 4531 Size: 416 53 83 399 24 1028 1128 958 Cuts at: 4531 442 Size: 1753 Fragments arranged by size: 1753 1128 1028 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2958 4502 1239 Size: 857 862 1544 2579 Fragments arranged by size: 2579 1544 862 857 Bpu10I CC'TnA_GC Cuts at: 2727 2727 Size: 5842 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2318 4505 882 Size: 617 819 2187 2219 Fragments arranged by size: 2219 2187 819 617 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3649 4031 4666 275 Size: 53 83 186 936 2116 382 635 1451 Fragments arranged by size: 2116 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4275 4673 4947 5094 5505 4275 Size: 398 274 147 411 4612 Fragments arranged by size: 4612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5617 Fragments arranged by size: 5617 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2156 1186 Size: 865 105 4872 Fragments arranged by size: 4872 865 105 BseYI C'CCAG_C Cuts at: 3102 5045 5541 3102 Size: 1943 496 3403 Fragments arranged by size: 3403 1943 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 4918 Fragments arranged by size: 4918 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 2218 3292 4053 5081 1540 Size: 275 186 21 196 1074 761 1028 2301 Fragments arranged by size: 2301 1074 1028 761 275 196 186 21 BsiHKAI G_wGCw'C Cuts at: 2449 2506 3903 3988 5059 2449 Size: 57 1397 85 1071 3232 Fragments arranged by size: 3232 1397 1071 85 57 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2449 2506 2842 3161 3903 3988 5059 Size: 399 659 57 336 319 742 85 1071 Cuts at: 5059 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 659 399 336 319 85 57 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 3197 844 Size: 1223 1130 3489 Fragments arranged by size: 3489 1223 1130 BsrBI CCG'CTC Cuts at: 1394 1823 3729 1394 Size: 429 1906 3507 Fragments arranged by size: 3507 1906 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4486 4673 1700 Size: 110 2676 187 2869 Fragments arranged by size: 2869 2676 187 110 BsrGI T'GTAC_A Cuts at: 96 2464 96 Size: 2368 3474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 3902 4914 3902 Size: 1012 4830 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2624 2890 3088 3172 3539 3924 3941 5393 Size: 652 266 198 84 367 385 17 1452 Cuts at: 5393 5479 5491 5837 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 652 385 367 346 266 198 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2966 487 Size: 683 56 1740 3363 Fragments arranged by size: 3363 1740 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2303 3358 4152 4172 2031 Size: 272 1055 794 20 3701 Fragments arranged by size: 3701 1055 794 272 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 2202 2850 3146 2202 Size: 648 296 4898 Fragments arranged by size: 4898 648 296 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2031 3289 Size: 54 1425 50 275 18 189 12 1258 Cuts at: 3289 4178 5807 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1258 889 275 189 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2019 3289 1537 Size: 275 207 1270 4090 Fragments arranged by size: 4090 1270 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 3009 3772 1353 Size: 1656 763 3423 Fragments arranged by size: 3423 1656 763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4429 4803 4949 387 Size: 1064 138 2840 374 146 1280 Fragments arranged by size: 2840 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2234 2958 3905 4502 5289 5557 Size: 393 464 138 724 947 597 787 268 Cuts at: 5557 1239 Size: 1524 Fragments arranged by size: 1524 947 787 724 597 464 393 268 138 EcoO109I rG'GnC_Cy Cuts at: 2056 2089 2056 Size: 33 5809 EcoRI G'AATT_C Cuts at: 3151 3529 3151 Size: 378 5464 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2465 2687 3047 2465 Size: 222 360 5260 Fragments arranged by size: 5260 360 222 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2407 4989 1678 Size: 729 2582 2531 Fragments arranged by size: 2582 2531 729 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4483 4515 4557 4589 1373 Size: 32 135 32 2911 32 42 32 2626 Fragments arranged by size: 2911 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2411 3186 3432 669 Size: 988 281 473 775 246 3079 Fragments arranged by size: 3079 988 775 473 281 246 HindIII A'AGCT_T Cuts at: 748 3068 748 Size: 2320 3522 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 5140 1305 Size: 3651 184 2007 Fragments arranged by size: 3651 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2123 3258 3935 5083 5328 1606 Size: 479 38 1135 677 1148 245 2120 Fragments arranged by size: 2120 1148 1135 677 479 245 38 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 2019 3289 2019 Size: 1270 4572 NspI r_CATG'y Cuts at: 1132 1229 3200 4745 1132 Size: 97 1971 1545 2229 Fragments arranged by size: 2229 1971 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 2435 1108 Size: 1327 4515 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2089 2056 Size: 33 5809 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5842 PvuII CAG'CTG Cuts at: 2123 2123 Size: 5842 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 3190 4325 5006 5197 826 Size: 219 2145 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5842 SmaI CCC'GGG Cuts at: 1872 3169 1872 Size: 1297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3946 4847 5109 820 Size: 197 926 2003 901 262 1553 Fragments arranged by size: 2003 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2762 3281 513 Size: 552 939 758 519 3074 Fragments arranged by size: 3074 939 758 552 519 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 3869 3886 4039 4224 4669 325 Size: 1255 783 1506 17 153 185 445 1498 Fragments arranged by size: 1506 1498 1255 783 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2464 4088 5800 Size: 274 80 33 51 1443 487 1624 1712 Cuts at: 5800 96 Size: 138 Fragments arranged by size: 1712 1624 1443 487 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2588 2620 5802 5834 2588 Size: 32 3182 32 2596 Fragments arranged by size: 3182 2596 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5842 XmaI C'CCGG_G Cuts at: 1870 3167 1870 Size: 1297 4545 XmnI GAAnn'nnTTC Cuts at: 2247 3971 2247 Size: 1724 4118 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3650 276 Size: 53 83 186 936 2116 2468 Fragments arranged by size: 2468 2116 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI