Restriction map for
pFN21AE4392
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AvaI AviII AvrII BamHI
BbeI BbrPI BciVI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI MaeI MfeI MflI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 1057 GGTACC G^GTACC
AfaI 1058 GTAC GT^AC
Asp700I 144 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1057 GGTACC G^GTACC
AspI 676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 702 TGATCA T^GATCA
BglI 1097 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 775 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 362 GGTCTC GGTCTC(1/5)
BsgI 160 GTGCAG GTGCAG(16/14)
BsmI 790 GAATGC GAATGC(1/-1)
BsrDI 1052 GCAATG GCAATG(2/0)
BsrFI 484 GCCGGC [GA]^CCGG[CT]
BssSI 937 CTCGTG CACGAG(-5/-1)
BstEII 171 GGTGACC G^GT[ACGT]ACC
BstPI 171 GGTGACC G^GT[ACGT]ACC
Cfr10I 484 GCCGGC [GA]^CCGG[CT]
EarI 266 CTCTTC CTCTTC(1/4)
EcoO65I 171 GGTGACC G^GT[ACGT]ACC
FbaI 702 TGATCA T^GATCA
KpnI 1057 GGTACC GGTAC^C
Ksp632I 266 CTCTTC CTCTTC(1/4)
MaeII 1169 ACGT A^CGT
MamI 775 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 484 GCCGGC GCC^GGC
NgoMIV 484 GCCGGC G^CCGGC
PflFI 676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 680 GAGTC GAGTC(4/5)
PshAI 148 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 956 CTGCAG CTGCA^G
RsaI 1058 GTAC GT^AC
TaqI 926 TCGA T^CGA
Tsp509I 870 AATT ^AATT
Tth111I 676 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 926 TCGA T^CGA
XmnI 144 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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BanI 709 GGTGCC G^G[CT][GA]CC
1057 GGTACC
BbsI 682 GTCTTC GAAGAC(2/6)
988 GTCTTC
BbvCI 693 CCTCAGC CCTCAGC(-5/-2)
280 GCTGAGG
BcgI 98 CGATCTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
263 GCACTCTTCTCG
BpuAI 682 GTCTTC GAAGAC(2/6)
988 GTCTTC
BseRI 691 CTCCTC GAGGAG(10/8)
1147 GAGGAG
BsrI 4 ACTGG ACTGG(1/-1)
1100 CCAGT
CfoI 25 GCGC GCG^C
720 GCGC
HaeII 24 GGCGCT [GA]GCGC^[CT]
719 GGCGCT
HgaI 38 GACGC GACGC(5/10)
663 GACGC
HhaI 25 GCGC GCG^C
720 GCGC
HinP1I 25 GCGC G^CGC
720 GCGC
KspI 49 CCGCGG CCGC^GG
256 CCGCGG
MslI 576 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1209 CATCTCCGTG
SacI 805 GAGCTC GAGCT^C
934 GAGCTC
SacII 49 CCGCGG CCGC^GG
256 CCGCGG
TfiI 54 GATTC G^A[AT]TC
555 GAATC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AccII AciI AluI AlwNI AspHI
AvaII BalI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BssKI BstF5I BstNI BstUI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI