(Circular) (Six-base) MAPSORT of: pFN21AE4392.seq Check: 6372 from: 1 to: 5950 pFN21AE4392 5950 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3293 1937 Size: 1356 4594 Acc65I G'GTAC_C Cuts at: 3062 3271 3062 Size: 209 5741 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2604 3037 4013 5397 5665 1632 Size: 972 433 976 1384 268 1917 Fragments arranged by size: 1917 1384 976 972 433 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 2585 1166 Size: 1419 4531 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 2046 2173 2969 3693 5265 2046 Size: 127 796 724 1572 2731 Fragments arranged by size: 2731 1572 796 724 127 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4504 160 Size: 872 3472 1606 Fragments arranged by size: 3472 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2714 3062 3271 4726 618 Size: 325 217 627 927 348 209 1455 1842 Fragments arranged by size: 1842 1455 927 627 348 325 217 209 BanII G_rGCy'C Cuts at: 1391 2609 2814 2943 3269 1391 Size: 1218 205 129 326 4072 Fragments arranged by size: 4072 1218 326 205 129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2680 2986 928 Size: 971 781 306 3892 Fragments arranged by size: 3892 971 781 306 BbvCI CC'TCA_GC Cuts at: 2286 2699 2286 Size: 413 5537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2092 2126 2257 2291 4141 4175 1903 Size: 34 155 34 131 34 1850 34 3678 Fragments arranged by size: 3678 1850 155 131 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3842 5052 3842 Size: 1210 4740 BclI T'GATC_A Cuts at: 1548 2707 1548 Size: 1159 4791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3108 4561 136 Size: 107 122 71 631 605 1436 1453 1525 Fragments arranged by size: 1525 1453 1436 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 3367 3367 Size: 5950 Bme1580I G_kGCm'C Cuts at: 1790 2270 4011 5167 1790 Size: 480 1741 1156 2573 Fragments arranged by size: 2573 1741 1156 480 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 3099 3681 Size: 416 53 83 399 24 602 1080 582 Cuts at: 3681 4639 442 Size: 958 1753 Fragments arranged by size: 1753 1080 958 602 582 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3232 3232 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2566 2693 3152 4610 1239 Size: 1327 127 459 1458 2579 Fragments arranged by size: 2579 1458 1327 459 127 Bpu10I CC'TnA_GC Cuts at: 2286 2699 2937 2286 Size: 413 238 5299 Fragments arranged by size: 5299 413 238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2373 4613 882 Size: 617 874 2240 2219 Fragments arranged by size: 2240 2219 874 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 2784 5944 2784 Size: 3160 2790 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3757 4139 4774 275 Size: 53 83 186 936 2224 382 635 1451 Fragments arranged by size: 2224 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2242 2272 2353 2383 2958 Size: 30 165 30 839 30 81 30 575 Cuts at: 2958 2988 1178 Size: 30 4140 Fragments arranged by size: 4140 839 575 165 81 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2687 3167 1186 Size: 1501 480 3969 Fragments arranged by size: 3969 1501 480 BseYI C'CCAG_C Cuts at: 2021 2046 3007 3165 5153 5649 2021 Size: 25 961 158 1988 496 2322 Fragments arranged by size: 2322 1988 961 496 158 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 1163 Size: 573 450 4927 Fragments arranged by size: 4927 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2482 2552 2814 2943 4011 4096 5167 2482 Size: 70 262 129 1068 85 1071 3265 Fragments arranged by size: 3265 1071 1068 262 129 85 70 BsmI GAATG_Cn' Cuts at: 2801 3461 3554 2801 Size: 660 93 5197 Fragments arranged by size: 5197 660 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2270 2482 2552 2609 2814 2943 3269 Size: 399 480 212 70 57 205 129 326 Cuts at: 3269 4011 4096 5167 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 326 212 205 129 85 70 57 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2205 2245 2677 3837 1394 Size: 429 382 40 432 1160 3507 Fragments arranged by size: 3507 1160 432 429 382 40 BsrDI GCAATG_nn' Cuts at: 65 1248 3064 4445 4619 65 Size: 1183 1816 1381 174 1396 Fragments arranged by size: 1816 1396 1381 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2489 4594 4781 1700 Size: 110 679 2105 187 2869 Fragments arranged by size: 2869 2105 679 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5950 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 2942 4010 5022 2942 Size: 1068 1012 3870 Fragments arranged by size: 3870 1068 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5950 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 2176 1184 Size: 992 4958 BstYI r'GATC_y Cuts at: 1972 3280 3647 4032 4049 5501 5587 5599 5945 Size: 1308 367 385 17 1452 86 12 346 Cuts at: 5945 1972 Size: 1977 Fragments arranged by size: 1977 1452 1308 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2054 2181 2261 2346 2633 2662 3219 Size: 552 989 127 80 85 287 29 557 Cuts at: 3219 513 Size: 3244 Fragments arranged by size: 3244 989 557 552 287 127 85 80 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2314 2451 2718 3058 487 Size: 683 56 1088 137 267 340 3379 Fragments arranged by size: 3379 1088 683 340 267 137 56 BtsI GCAGTG_nn' Cuts at: 2860 2866 3466 4260 4280 2860 Size: 6 600 794 20 4530 Fragments arranged by size: 4530 794 600 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2177 2212 529 Size: 35 1613 35 4267 Fragments arranged by size: 4267 1613 35 35 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 3095 3222 Size: 54 1425 50 275 18 829 436 127 Cuts at: 3222 3397 4286 5915 8 Size: 175 889 1629 43 Fragments arranged by size: 1629 1425 889 829 436 275 175 127 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2277 3880 1353 Size: 924 1603 3423 Fragments arranged by size: 3423 1603 924 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3088 4537 4911 5057 387 Size: 1064 138 1499 1449 374 146 1280 Fragments arranged by size: 1499 1449 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 2941 2812 Size: 129 5821 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2566 2604 2693 3037 3152 4013 4610 Size: 393 934 38 89 344 115 861 597 Cuts at: 4610 5397 5665 1239 Size: 787 268 1524 Fragments arranged by size: 1524 934 861 787 597 393 344 268 115 89 38 EcoO109I rG'GnC_Cy Cuts at: 2913 3101 3160 2913 Size: 188 59 5703 Fragments arranged by size: 5703 188 59 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 2033 2728 5097 1678 Size: 355 695 2369 2531 Fragments arranged by size: 2531 2369 695 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2958 2990 4591 4623 4665 Size: 32 135 32 1386 32 1601 32 42 Cuts at: 4665 4697 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 1386 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3066 3275 3066 Size: 209 5741 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3027 5064 5248 1305 Size: 1722 2037 184 2007 Fragments arranged by size: 2037 2007 1722 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2661 3097 3224 10 Size: 54 1425 343 829 436 127 2736 Fragments arranged by size: 2736 1425 829 436 343 127 54 MslI CAynn'nnrTG Cuts at: 518 1166 2585 3218 3908 4267 4426 518 Size: 648 1419 633 690 359 159 2042 Fragments arranged by size: 2042 1419 690 648 633 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2173 2263 2626 2745 3366 4043 5191 Size: 450 117 90 363 119 621 677 1148 Cuts at: 5191 5436 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 621 450 363 245 119 117 90 NaeI GCC'GGC Cuts at: 2491 2491 Size: 5950 NcoI C'CATG_G Cuts at: 513 1065 2181 2346 2662 513 Size: 552 1116 165 316 3801 Fragments arranged by size: 3801 1116 552 316 165 NdeI CA'TA_TG Cuts at: 387 387 Size: 5950 NgoMIV G'CCGG_C Cuts at: 2489 2489 Size: 5950 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NspI r_CATG'y Cuts at: 1132 1229 3308 4853 1132 Size: 97 2079 1545 2229 Fragments arranged by size: 2229 2079 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5950 PfoI T'CCnGG_A Cuts at: 2034 2543 2034 Size: 509 5441 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2157 1613 Size: 544 5406 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 2965 3302 830 Size: 2135 337 3478 Fragments arranged by size: 3478 2135 337 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 SacI G_AGCT'C Cuts at: 2814 2943 2814 Size: 129 5821 SacII CC_GC'GG Cuts at: 2057 2264 2057 Size: 207 5743 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2080 2373 2961 2970 3298 4433 5114 Size: 219 1035 293 588 9 328 1135 681 Cuts at: 5114 5305 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1035 681 588 328 293 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 3874 5 Size: 47 3822 2081 Fragments arranged by size: 3822 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2139 2181 2346 2419 2662 3389 513 Size: 552 1074 42 165 73 243 727 3074 Fragments arranged by size: 3074 1074 727 552 243 165 73 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 2147 2188 3977 3994 4147 4332 Size: 1255 500 67 41 1789 17 153 185 Cuts at: 4332 4777 325 Size: 445 1498 Fragments arranged by size: 1789 1498 1255 500 445 185 153 67 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4196 5908 96 Size: 274 80 33 51 1443 2219 1712 138 Fragments arranged by size: 2219 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2242 2274 2353 2385 5910 5942 2242 Size: 32 79 32 3525 32 2250 Fragments arranged by size: 3525 2250 79 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2684 1316 Size: 1368 4582 XbaI T'CTAG_A Cuts at: 3286 3286 Size: 5950 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2153 4079 2153 Size: 1926 4024 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI