(Linear) (Six-base) MAPSORT of: KIEE4392.seq Check: 2722 from: 1 to: 1245 KIEE4392.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. Acc65I G'GTAC_C Cuts at: 0 1057 1245 Size: 1057 188 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 599 1032 1245 Size: 599 433 213 Fragments arranged by size: 599 433 213 AleI CACnn'nnGTG Cuts at: 0 580 1245 Size: 580 665 AlwNI CAG_nnn'CTG Cuts at: 0 41 168 964 1245 Size: 41 127 796 281 Fragments arranged by size: 796 281 127 41 BanI G'GyrC_C Cuts at: 0 709 1057 1245 Size: 709 348 188 Fragments arranged by size: 709 348 188 BanII G_rGCy'C Cuts at: 0 604 809 938 1245 Size: 604 205 129 307 Fragments arranged by size: 604 307 205 129 BbsI GAAGACnn'nnnn_ Cuts at: 0 675 981 1245 Size: 675 306 264 Fragments arranged by size: 675 306 264 BbvCI CC'TCA_GC Cuts at: 0 281 694 1245 Size: 281 413 551 Fragments arranged by size: 551 413 281 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 87 121 252 286 1245 Size: 87 34 131 34 959 Fragments arranged by size: 959 131 87 34 34 BclI T'GATC_A Cuts at: 0 702 1245 Size: 702 543 BglI GCCn_nnn'nGGC Cuts at: 0 1103 1245 Size: 1103 142 Bme1580I G_kGCm'C Cuts at: 0 265 1245 Size: 265 980 BmrI ACTGGGnnnn_n' Cuts at: 0 14 1094 1245 Size: 14 1080 151 Fragments arranged by size: 1080 151 14 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1227 1245 Size: 1227 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 688 1147 1245 Size: 561 127 459 98 Fragments arranged by size: 561 459 127 98 Bpu10I CC'TnA_GC Cuts at: 0 281 694 932 1245 Size: 281 413 238 313 Fragments arranged by size: 413 313 281 238 BsaI GGTCTCn'nnnn_ Cuts at: 0 368 1245 Size: 368 877 BsaBI GATnn'nnATC Cuts at: 0 779 1245 Size: 779 466 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 237 267 348 378 953 983 1245 Size: 237 30 81 30 575 30 262 Fragments arranged by size: 575 262 237 81 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 682 1162 1245 Size: 682 480 83 Fragments arranged by size: 682 480 83 BseYI C'CCAG_C Cuts at: 0 16 41 1002 1160 1245 Size: 16 25 961 158 85 Fragments arranged by size: 961 158 85 25 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 1245 Size: 181 1064 BsiHKAI G_wGCw'C Cuts at: 0 477 547 809 938 1245 Size: 477 70 262 129 307 Fragments arranged by size: 477 307 262 129 70 BsmI GAATG_Cn' Cuts at: 0 796 1245 Size: 796 449 Bsp1286I G_dGCh'C Cuts at: 0 265 477 547 604 809 938 1245 Size: 265 212 70 57 205 129 307 Fragments arranged by size: 307 265 212 205 129 70 57 BsrBI CCG'CTC Cuts at: 0 200 240 672 1245 Size: 200 40 432 573 Fragments arranged by size: 573 432 200 40 BsrDI GCAATG_nn' Cuts at: 0 1059 1245 Size: 1059 186 BsrFI r'CCGG_y Cuts at: 0 484 1245 Size: 484 761 BssSI C'ACGA_G Cuts at: 0 937 1245 Size: 937 308 BstEII G'GTnAC_C Cuts at: 0 171 1245 Size: 171 1074 BtgI C'CryG_G Cuts at: 0 49 176 256 341 628 657 1214 1245 Size: 49 127 80 85 287 29 557 31 Fragments arranged by size: 557 287 127 85 80 49 31 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 309 446 713 1053 1245 Size: 309 137 267 340 192 Fragments arranged by size: 340 309 267 192 137 BtsI GCAGTG_nn' Cuts at: 0 855 861 1245 Size: 855 6 384 Fragments arranged by size: 855 384 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 172 207 1245 Size: 172 35 1038 Fragments arranged by size: 1038 172 35 EaeI y'GGCC_r Cuts at: 0 654 1090 1217 1245 Size: 654 436 127 28 Fragments arranged by size: 654 436 127 28 EarI CTCTTCn'nnn_ Cuts at: 0 272 1245 Size: 272 973 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1083 1245 Size: 1083 162 EcoICRI GAG'CTC Cuts at: 0 807 936 1245 Size: 807 129 309 Fragments arranged by size: 807 309 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 599 688 1032 1147 1245 Size: 561 38 89 344 115 98 Fragments arranged by size: 561 344 115 98 89 38 EcoO109I rG'GnC_Cy Cuts at: 0 908 1096 1155 1245 Size: 908 188 59 90 Fragments arranged by size: 908 188 90 59 HaeII r_GCGC'y Cuts at: 0 28 723 1245 Size: 28 695 522 Fragments arranged by size: 695 522 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 953 985 1245 Size: 953 32 260 Fragments arranged by size: 953 260 32 KpnI G_GTAC'C Cuts at: 0 1061 1245 Size: 1061 184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1022 1245 Size: 1022 223 MscI TGG'CCA Cuts at: 0 656 1092 1219 1245 Size: 656 436 127 26 Fragments arranged by size: 656 436 127 26 MslI CAynn'nnrTG Cuts at: 0 580 1213 1245 Size: 580 633 32 Fragments arranged by size: 633 580 32 MspA1I CmG'CkG Cuts at: 0 51 168 258 621 740 1245 Size: 51 117 90 363 119 505 Fragments arranged by size: 505 363 119 117 90 51 NaeI GCC'GGC Cuts at: 0 486 1245 Size: 486 759 NcoI C'CATG_G Cuts at: 0 176 341 657 1245 Size: 176 165 316 588 Fragments arranged by size: 588 316 176 165 NgoMIV G'CCGG_C Cuts at: 0 484 1245 Size: 484 761 PfoI T'CCnGG_A Cuts at: 0 29 538 1245 Size: 29 509 707 Fragments arranged by size: 707 509 29 PshAI GACnn'nnGTC Cuts at: 0 152 1245 Size: 152 1093 PstI C_TGCA'G Cuts at: 0 960 1245 Size: 960 285 SacI G_AGCT'C Cuts at: 0 809 938 1245 Size: 809 129 307 Fragments arranged by size: 809 307 129 SacII CC_GC'GG Cuts at: 0 52 259 1245 Size: 52 207 986 Fragments arranged by size: 986 207 52 SfcI C'TryA_G Cuts at: 0 75 368 956 965 1245 Size: 75 293 588 9 280 Fragments arranged by size: 588 293 280 75 9 StyI C'CwwG_G Cuts at: 0 134 176 341 414 657 1245 Size: 134 42 165 73 243 588 Fragments arranged by size: 588 243 165 134 73 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 75 142 183 1245 Size: 75 67 41 1062 Fragments arranged by size: 1062 75 67 41 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 237 269 348 380 1245 Size: 237 32 79 32 865 Fragments arranged by size: 865 237 79 32 32 Tth111I GACn'n_nGTC Cuts at: 0 679 1245 Size: 679 566 XmnI GAAnn'nnTTC Cuts at: 0 148 1245 Size: 148 1097 Enzymes that do cut: Acc65I AcuI AleI AlwNI BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BssSI BstEII BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PfoI PshAI PstI SacI SacII SfcI StyI TaqII TstI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmaI ZraI