Restriction map for
pFN21AE1152
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MluI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 698 TCCGGA T^CCGGA
Asp700I 295 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 842 CCCGGG C^[CT]CG[GA]G
BalI 157 TGGCCA TGG^CCA
BbsI 409 GAAGAC GAAGAC(2/6)
BglII 420 AGATCT A^GATCT
BpmI 50 CTGGAG CTGGAG(16/14)
BpuAI 409 GAAGAC GAAGAC(2/6)
BseAI 698 TCCGGA T^CCGGA
BsmAI 1296 GAGAC GTCTC(1/5)
BsmI 174 GAATGC GAATGC(1/-1)
BsoBI 842 CCCGGG C^[CT]CG[GA]G
BspEI 698 TCCGGA T^CCGGA
BstXI 1424 CCAGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 420 AGATCT [GA]^GATC[CT]
DraI 678 TTTAAA TTT^AAA
DraII 194 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 157 TGGCCA [CT]^GGCC[GA]
EcoNI 186 CCTTTCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 194 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 500 CCTTGG C^C[AT][AT]GG
HincII 958 GTCAAC GT[CT]^[GA]AC
HindII 958 GTCAAC GT[CT]^[GA]AC
MfeI 1328 CAATTG C^AATTG
MflI 420 AGATCT [GA]^GATC[CT]
MluNI 157 TGGCCA TGG^CCA
MroI 698 TCCGGA T^CCGGA
MscI 157 TGGCCA TGG^CCA
MspA1I 980 CAGCTG C[AC]G^C[GT]G
MunI 1328 CAATTG C^AATTG
PpuMI 194 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 980 CAGCTG CAG^CTG
SfaNI 579 GCATC GCATC(5/9)
SmaI 842 CCCGGG CCC^GGG
SspI 389 AATATT AAT^ATT
StyI 500 CCTTGG C^C[AT][AT]GG
TaqI 293 TCGA T^CGA
TthHB8I 293 TCGA T^CGA
XcmI 160 CCAGCACGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 420 AGATCT [GA]^GATC[CT]
XmaCI 842 CCCGGG C^CCGGG
XmaI 842 CCCGGG C^CCGGG
XmnI 295 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 482 GTAC GT^AC
559 GTAC
AflIII 69 ACATGT A^C[GA][CT]GT
268 ACATGT
AlwI 787 GATCC GGATC(4/5)
831 GGATC
AlwNI 756 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
912 CAGAGCCTG
AspHI 243 GAGCAC G[AT]GC[AT]^C
1054 GAGCAC
BanII 506 GAGCCC G[GA]GC[CT]^C
729 GAGCCC
BcnI 842 CCCGG CC^[GC]GG
843 CCGGG
BsaAI 277 TACGTA [CT]AC^GT[GA]
772 TACGTA
BsaWI 698 TCCGGA [AT]^CCGG[AT]
1143 ACCGGA
BseRI 1364 CTCCTC GAGGAG(10/8)
1217 GAGGAG
BsiHKAI 243 GAGCAC G[AT]GC[AT]^C
1054 GAGCAC
BspLU11I 69 ACATGT A^CATGT
268 ACATGT
BspMI 126 ACCTGC ACCTGC(4/8)
192 GCAGGT
MwoI 40 GCGGCTGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
982 GCTGTTTCTGC
NciI 842 CCCGG CC^[GC]GG
843 CCGGG
PleI 529 GACTC GAGTC(4/5)
1362 GACTC
PstI 890 CTGCAG CTGCA^G
1005 CTGCAG
RsaI 482 GTAC GT^AC
559 GTAC
SnaBI 277 TACGTA TAC^GTA
772 TACGTA
Tsp45I 317 GTGAC ^GT[GC]AC
793 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaII BbvI BfaI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV NspI PflMI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I TseI Tsp509I TspRI Van91I