Restriction map for
pFN21AE6322
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BseRI BsgI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 704 GGTACC G^GTACC
AccI 633 GTCGAC GT^[AC][GT]AC
Asp718I 704 GGTACC G^GTACC
AspHI 673 GAGCTC G[AT]GC[AT]^C
BalI 413 TGGCCA TGG^CCA
BamHI 86 GGATCC G^GATCC
BcgI 635 CGACATTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 182 TGATCA T^GATCA
BcnI 245 CCCGG CC^[GC]GG
BfaI 8 CTAG C^TAG
BglI 24 GCCCGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 203 AGATCT A^GATCT
BsaBI 204 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 294 GGTCTC GGTCTC(1/5)
BsiEI 27 CGACCG CG[GA][CT]^CG
BsiHKAI 673 GAGCTC G[AT]GC[AT]^C
BsmBI 139 GAGACG CGTCTC(1/5)
BsmFI 794 GTCCC GGGAC(10/14)
Bsp1407I 464 TGTACA T^GTACA
BspMI 802 ACCTGC ACCTGC(4/8)
BsrBI 43 CCGCTC CCGCTC(-3/-3)
BsrDI 638 CATTGC GCAATG(2/0)
BsrFI 29 ACCGGC [GA]^CCGG[CT]
BsrGI 464 TGTACA T^GTACA
BstEII 384 GGTGACC G^GT[ACGT]ACC
BstPI 384 GGTGACC G^GT[ACGT]ACC
BstXI 746 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 36 GCGC GCG^C
Cfr10I 29 ACCGGC [GA]^CCGG[CT]
DraII 338 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 290 CCATGG C^C[GA][CT]GG
EaeI 413 TGGCCA [CT]^GGCC[GA]
EarI 859 CTCTTC CTCTTC(1/4)
EcoO109I 338 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 384 GGTGACC G^GT[ACGT]ACC
FauI 579 GCGGG CCCGC(4/6)
FbaI 182 TGATCA T^GATCA
HhaI 36 GCGC GCG^C
HinP1I 36 GCGC G^CGC
HinfI 656 GAATC G^A[ACGT]TC
KpnI 704 GGTACC GGTAC^C
Ksp632I 859 CTCTTC CTCTTC(1/4)
MaeI 8 CTAG C^TAG
MaeII 142 ACGT A^CGT
MaeIII 385 GTGAC ^GT[ACGT]AC
MamI 204 GATCTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MslI 768 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 245 CCCGG CC^[GC]GG
NcoI 290 CCATGG C^CATGG
NspI 575 ACATGC [GA]CATG^[CT]
PflMI 881 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 338 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 91 CTGCAG CTGCA^G
SacI 673 GAGCTC GAGCT^C
SalI 633 GTCGAC G^TCGAC
SbfI 90 CCTGCAGG CCTGCA^GG
SmlI 687 CTTGAG C^T[CT][GA]AG
Sse8387I 90 CCTGCAGG CCTGCA^GG
SspBI 464 TGTACA T^GTACA
TfiI 656 GAATC G^A[AT]TC
Tsp45I 385 GTGAC ^GT[GC]AC
Van91I 881 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 881 CCATGCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 43 CCGC CCGC(-3/-1)
579 GCGG
BanII 581 GGGCTC G[GA]GC[CT]^C
673 GAGCTC
BbsI 954 GAAGAC GAAGAC(2/6)
166 GTCTTC
BpmI 297 CTCCAG CTGGAG(16/14)
446 CTGGAG
BpuAI 954 GAAGAC GAAGAC(2/6)
166 GTCTTC
BsmAI 139 GAGAC GTCTC(1/5)
295 GTCTC
BstYI 86 GGATCC [GA]^GATC[CT]
203 AGATCT
DdeI 335 CTCAG C^T[ACGT]AG
950 CTAAG
HapII 30 CCGG C^CGG
246 CCGG
HincII 322 GTCAAC GT[CT]^[GA]AC
633 GTCGAC
HindII 322 GTCAAC GT[CT]^[GA]AC
633 GTCGAC
HpaII 30 CCGG C^CGG
246 CCGG
HphI 384 GGTGA GGTGA(8/7)
146 TCACC
MflI 86 GGATCC [GA]^GATC[CT]
203 AGATCT
MseI 157 TTAA T^TAA
436 TTAA
MspI 30 CCGG C^CGG
246 CCGG
SfaNI 612 GATGC GCATC(5/9)
928 GATGC
SfcI 91 CTGCAG C^T[GA][CT]AG
313 CTACAG
TaqI 634 TCGA T^CGA
825 TCGA
Tru9I 157 TTAA T^TAA
436 TTAA
TthHB8I 634 TCGA T^CGA
825 TCGA
XhoII 86 GGATCC [GA]^GATC[CT]
203 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AlwI AvaII BanI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI StyI TseI Tsp509I TspRI