################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AhdI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HindIII KpnI KspI MamI MflI MluI MluNI MroI MscI MvnI NaeI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 3 GGCGCC G[GA]^CG[CT]C AgeI 1062 ACCGGT A^CCGGT Alw44I 578 GTGCAC G^TGCAC ApaLI 578 GTGCAC G^TGCAC AseI 508 ATTAAT AT^TAAT AsnI 508 ATTAAT AT^TAAT Asp700I 360 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 3 GGCGCC GGCGC^C BcnI 1098 CCCGG CC^[GC]GG BpmI 1723 CTCCAG CTGGAG(16/14) BsaHI 3 GGCGCC G[GA]^CG[CT]C BsaWI 1062 ACCGGT [AT]^CCGG[AT] BsgI 208 CTGCAC GTGCAG(16/14) BsmAI 1567 GTCTC GTCTC(1/5) Bsp1407I 47 TGTACA T^GTACA BspMI 1227 GCAGGT ACCTGC(4/8) BsrBI 16 CCGCTC CCGCTC(-3/-3) BsrFI 1062 ACCGGT [GA]^CCGG[CT] BsrGI 47 TGTACA T^GTACA CfoI 4 GCGC GCG^C Cfr10I 1062 ACCGGT [GA]^CCGG[CT] EarI 1387 GAAGAG CTCTTC(1/4) EcoT14I 94 CCAAGG C^C[AT][AT]GG FauI 428 CCCGC CCCGC(4/6) HaeII 3 GGCGCC [GA]GCGC^[CT] HaeIII 820 GGCC GG^CC HhaI 4 GCGC GCG^C Hin1I 3 GGCGCC G[GA]^CG[CT]C HinP1I 4 GCGC G^CGC HpaI 436 GTTAAC GTT^AAC KasI 3 GGCGCC G^GCGCC Ksp632I 1387 GAAGAG CTCTTC(1/4) MaeII 274 ACGT A^CGT MfeI 479 CAATTG C^AATTG MslI 1192 CACAAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 308 CAGCTG C[AC]G^C[GT]G MunI 479 CAATTG C^AATTG NarI 3 GGCGCC GG^CGCC NciI 1098 CCCGG CC^[GC]GG NheI 715 GCTAGC G^CTAGC PinAI 1062 ACCGGT A^CCGGT PleI 1515 GAGTC GAGTC(4/5) PshAI 1510 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 508 ATTAAT AT^TAAT PvuII 308 CAGCTG CAG^CTG SfoI 3 GGCGCC GGC^GCC SspBI 47 TGTACA T^GTACA StyI 94 CCAAGG C^C[AT][AT]GG XmnI 360 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 16 CCGC CCGC(-3/-1) 429 CCGC AflIII 352 ACATGT A^C[GA][CT]GT 1126 ACATGT AlwI 919 GATCC GGATC(4/5) 34 GGATC AlwNI 1661 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG 1726 CAGGAGCTG BciVI 314 GGATAC GTATCC(6/5) 1156 GGATAC BspLU11I 352 ACATGT A^CATGT 1126 ACATGT DpnII 35 GATC ^GATC 919 GATC DraII 142 GGGACCT [GA]G^G[ACGT]CC[CT] 1586 GGGACCC EcoNI 222 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1719 CCTTCTCCAGG EcoO109I 142 GGGACCT [GA]G^G[ACGT]CC[CT] 1586 GGGACCC EcoRI 244 GAATTC G^AATTC 843 GAATTC HapII 1063 CCGG C^CGG 1099 CCGG HincII 436 GTTAAC GT[CT]^[GA]AC 727 GTTGAC HindII 436 GTTAAC GT[CT]^[GA]AC 727 GTTGAC HpaII 1063 CCGG C^CGG 1099 CCGG MboI 35 GATC ^GATC 919 GATC MspI 1063 CCGG C^CGG 1099 CCGG NdeII 35 GATC ^GATC 919 GATC NspI 352 ACATGT [GA]CATG^[CT] 1126 ACATGT PflMI 811 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1725 CCAGGAGCTGG PpuMI 142 GGGACCT [GA]G^G[AT]CC[CT] 1586 GGGACCC Sau3AI 35 GATC ^GATC 919 GATC SfcI 797 CTGTAG C^T[GA][CT]AG 963 CTATAG SspI 178 AATATT AAT^ATT 1257 AATATT TaqI 218 TCGA T^CGA 1640 TCGA TfiI 100 GAATC G^A[AT]TC 1075 GATTC Tsp45I 190 GTCAC ^GT[GC]AC 1508 GTGAC TthHB8I 218 TCGA T^CGA 1640 TCGA Van91I 811 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1725 CCAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI AvaII BanI BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboII MnlI MseI MvaI MwoI NlaIII NlaIV RsaI Sau96I ScrFI Tru9I TseI Tsp509I TspRI