(Circular) (Six-base) MAPSORT of: pFN21AE1363.seq Check: 8119 from: 1 to: 6460 pFN21AE1363 6460 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4270 278 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3803 1937 Size: 1866 4594 Acc65I G'GTAC_C Cuts at: 3781 3781 Size: 6460 AclI AA'CG_TT Cuts at: 1563 4587 4960 1563 Size: 3024 373 3063 Fragments arranged by size: 3063 3024 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 4523 5907 6175 1632 Size: 620 2271 1384 268 1917 Fragments arranged by size: 2271 1917 1384 620 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6263 AflIII A'CryG_T Cuts at: 1203 2357 3131 5359 6336 1203 Size: 1154 774 2228 977 1327 Fragments arranged by size: 2228 1327 1154 977 774 AgeI A'CCGG_T Cuts at: 3067 5291 3067 Size: 2224 4236 AhdI GACnn_n'nnGTC Cuts at: 5189 5189 Size: 6460 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6460 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6460 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2839 2871 2839 Size: 32 6428 AlwNI CAG_nnn'CTG Cuts at: 3671 3736 4203 5775 3671 Size: 65 467 1572 4356 Fragments arranged by size: 4356 1572 467 65 ApaLI G'TGCA_C Cuts at: 2583 4517 5673 2583 Size: 1934 1156 3370 Fragments arranged by size: 3370 1934 1156 ApoI r'AATT_y Cuts at: 1493 2249 2848 3168 3705 3769 3995 4147 6256 Size: 756 599 320 537 64 226 152 2109 Cuts at: 6256 1493 Size: 1697 Fragments arranged by size: 2109 1697 756 599 537 320 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 2514 5014 160 Size: 872 1482 2500 1606 Fragments arranged by size: 2500 1606 1482 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 AvaI C'yCGr_G Cuts at: 1870 1943 3785 1870 Size: 73 1842 4545 Fragments arranged by size: 4545 1842 73 BamHI G'GATC_C Cuts at: 3790 4157 3790 Size: 367 6093 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 3099 3532 3781 5236 Size: 325 217 627 221 1091 433 249 1455 Cuts at: 5236 618 Size: 1842 Fragments arranged by size: 1842 1455 1091 627 433 325 249 221 217 BanII G_rGCy'C Cuts at: 1391 3779 1391 Size: 2388 4072 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6460 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5489 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4651 4685 1903 Size: 34 2714 34 3678 Fragments arranged by size: 3678 2714 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2313 3155 4352 5562 2313 Size: 842 1197 1210 3211 Fragments arranged by size: 3211 1210 1197 842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6460 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5071 136 Size: 107 122 71 631 605 3399 1525 Fragments arranged by size: 3399 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6455 6455 Size: 6460 BlpI GC'TnA_GC Cuts at: 3877 3877 Size: 6460 Bme1580I G_kGCm'C Cuts at: 1790 2434 2587 3104 4521 5677 1790 Size: 644 153 517 1417 1156 2573 Fragments arranged by size: 2573 1417 1156 644 517 153 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6460 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2361 4191 5149 Size: 416 53 83 399 24 944 1830 958 Cuts at: 5149 442 Size: 1753 Fragments arranged by size: 1830 1753 958 944 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2724 1056 Size: 1668 4792 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3713 5120 1239 Size: 2474 1407 2579 Fragments arranged by size: 2579 2474 1407 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4585 5450 5748 4585 Size: 865 298 5297 Fragments arranged by size: 5297 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5123 882 Size: 617 3624 2219 Fragments arranged by size: 3624 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4251 493 Size: 711 3047 2702 Fragments arranged by size: 3047 2702 711 BsaBI GATnn'nnATC Cuts at: 6454 6454 Size: 6460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4267 4649 5284 Size: 53 83 186 936 476 2258 382 635 Cuts at: 5284 275 Size: 1451 Fragments arranged by size: 2258 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3067 4893 5291 5565 5712 6123 3067 Size: 1826 398 274 147 411 3404 Fragments arranged by size: 3404 1826 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2163 2193 3121 3151 1178 Size: 30 165 30 760 30 928 30 4487 Fragments arranged by size: 4487 928 760 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6460 BseYI C'CCAG_C Cuts at: 2315 3349 5663 6159 2315 Size: 1034 2314 496 2616 Fragments arranged by size: 2616 2314 1034 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 1163 Size: 573 462 5425 Fragments arranged by size: 5425 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3910 4671 5699 1540 Size: 275 186 1909 761 1028 2301 Fragments arranged by size: 2301 1909 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2587 3139 3557 4521 4606 5677 2587 Size: 552 418 964 85 1071 3370 Fragments arranged by size: 3370 1071 964 552 418 85 BsmI GAATG_Cn' Cuts at: 3971 4064 3971 Size: 93 6367 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2434 2587 3104 3139 3557 3779 4521 Size: 399 644 153 517 35 418 222 742 Cuts at: 4521 4606 5677 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 517 418 399 222 153 85 35 BspEI T'CCGG_A Cuts at: 6123 6123 Size: 6460 BspHI T'CATG_A Cuts at: 4349 6079 4349 Size: 1730 4730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3223 3815 844 Size: 2379 592 3489 Fragments arranged by size: 3489 2379 592 BsrBI CCG'CTC Cuts at: 1394 1823 2023 4347 1394 Size: 429 200 2324 3507 Fragments arranged by size: 3507 2324 429 200 BsrDI GCAATG_nn' Cuts at: 65 1248 2173 2443 2778 4955 5129 65 Size: 1183 925 270 335 2177 174 1396 Fragments arranged by size: 2177 1396 1183 925 335 270 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3067 5104 5291 1700 Size: 110 1257 2037 187 2869 Fragments arranged by size: 2869 2037 1257 187 110 BsrGI T'GTAC_A Cuts at: 96 2052 96 Size: 1956 4504 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6460 BssSI C'ACGA_G Cuts at: 4520 5532 4520 Size: 1012 5448 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6460 BstBI TT'CG_AA Cuts at: 5264 5264 Size: 6460 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6460 BstYI r'GATC_y Cuts at: 1972 3790 4157 4542 4559 6011 6097 6109 6455 Size: 1818 367 385 17 1452 86 12 346 Cuts at: 6455 1972 Size: 1977 Fragments arranged by size: 1977 1818 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4969 Fragments arranged by size: 4969 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5721 Fragments arranged by size: 5721 683 56 BtsI GCAGTG_nn' Cuts at: 3976 4770 4790 3976 Size: 794 20 5646 Fragments arranged by size: 5646 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6425 DraI TTT'AAA Cuts at: 3764 3764 Size: 6460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5467 809 Size: 507 4151 1802 Fragments arranged by size: 4151 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3907 4796 6425 Size: 54 1425 50 275 18 2077 889 1629 Cuts at: 6425 8 Size: 43 Fragments arranged by size: 2077 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3907 1537 Size: 275 2095 4090 Fragments arranged by size: 4090 2095 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3387 4390 1353 Size: 2034 1003 3423 Fragments arranged by size: 3423 2034 1003 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5047 5421 5567 387 Size: 1064 138 3458 374 146 1280 Fragments arranged by size: 3458 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 3713 4523 5120 5907 6175 1239 Size: 393 620 1461 810 597 787 268 1524 Fragments arranged by size: 1524 1461 810 787 620 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2231 3728 2231 Size: 1497 4963 EcoO109I rG'GnC_Cy Cuts at: 2148 3592 2148 Size: 1444 5016 EcoRI G'AATT_C Cuts at: 2249 2848 3769 4147 2249 Size: 599 921 378 4562 Fragments arranged by size: 4562 921 599 378 FspI TGC'GCA Cuts at: 4966 4966 Size: 6460 HaeII r_GCGC'y Cuts at: 1678 2012 5607 1678 Size: 334 3595 2531 Fragments arranged by size: 3595 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3197 3229 5101 5133 5175 Size: 32 135 32 1625 32 1872 32 42 Cuts at: 5175 5207 1373 Size: 32 2626 Fragments arranged by size: 2626 1872 1625 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2443 2734 3804 4050 669 Size: 988 281 505 291 1070 246 3079 Fragments arranged by size: 3079 1070 988 505 291 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6460 HpaI GTT'AAC Cuts at: 2443 4050 2443 Size: 1607 4853 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6460 KpnI G_GTAC'C Cuts at: 3785 3785 Size: 6460 MfeI C'AATT_G Cuts at: 2484 4059 2484 Size: 1575 4885 MluI A'CGCG_T Cuts at: 6336 6336 Size: 6460 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3678 5574 5758 1305 Size: 2373 1896 184 2007 Fragments arranged by size: 2373 2007 1896 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4638 Fragments arranged by size: 4638 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3201 4418 4777 4936 518 Size: 648 2035 1217 359 159 2042 Fragments arranged by size: 2042 2035 1217 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2315 3876 4553 5701 5946 1606 Size: 709 1561 677 1148 245 2120 Fragments arranged by size: 2120 1561 1148 709 677 245 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6460 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4969 Fragments arranged by size: 4969 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6460 NheI G'CTAG_C Cuts at: 1052 2720 1052 Size: 1668 4792 NotI GC'GGCC_GC Cuts at: 3907 3907 Size: 6460 NspI r_CATG'y Cuts at: 1132 1229 2361 3135 3818 5363 1132 Size: 97 1132 774 683 1545 2229 Fragments arranged by size: 2229 1545 1132 774 683 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6460 PciI A'CATG_T Cuts at: 2357 3131 5359 2357 Size: 774 2228 3458 Fragments arranged by size: 3458 2228 774 PflMI CCAn_nnn'nTGG Cuts at: 1108 2822 3736 1108 Size: 1714 914 3832 Fragments arranged by size: 3832 1714 914 PfoI T'CCnGG_A Cuts at: 2569 3729 2569 Size: 1160 5300 PmeI GTTT'AAAC Cuts at: 3764 3764 Size: 6460 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2935 2967 4529 4561 6042 6074 2935 Size: 32 1562 32 1481 32 3321 Fragments arranged by size: 3321 1562 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 3592 2148 Size: 1444 5016 PshAI GACnn'nnGTC Cuts at: 1613 3519 1613 Size: 1906 4554 PsiI TTA'TAA Cuts at: 4030 4030 Size: 6460 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2754 2786 2754 Size: 32 6428 PstI C_TGCA'G Cuts at: 830 3812 830 Size: 2982 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6460 PvuII CAG'CTG Cuts at: 2315 2315 Size: 6460 SalI G'TCGA_C Cuts at: 1936 3802 1936 Size: 1866 4594 SanDI GG'GwC_CC Cuts at: 3592 3592 Size: 6460 SbfI CC_TGCA'GG Cuts at: 3812 3812 Size: 6460 ScaI AGT'ACT Cuts at: 4708 6420 4708 Size: 1712 4748 SfcI C'TryA_G Cuts at: 826 1045 2802 2968 3808 4943 5624 5815 826 Size: 219 1757 166 840 1135 681 191 1471 Fragments arranged by size: 1757 1471 1135 840 681 219 191 166 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6460 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6460 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6460 SmaI CCC'GGG Cuts at: 1872 3787 1872 Size: 1915 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4564 5465 5727 820 Size: 197 926 2621 901 262 1553 Fragments arranged by size: 2621 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4251 493 Size: 3758 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6460 SphI G_CATG'C Cuts at: 1132 3818 1132 Size: 2686 3774 SspI AAT'ATT Cuts at: 5 52 2185 3264 4384 5 Size: 47 2133 1079 1120 2081 Fragments arranged by size: 2133 2081 1120 1079 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6460 StyI C'CwwG_G Cuts at: 513 1065 2004 2099 3899 513 Size: 552 939 95 1800 3074 Fragments arranged by size: 3074 1800 939 552 95 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2272 4487 4504 4657 4842 5287 325 Size: 1255 692 2215 17 153 185 445 1498 Fragments arranged by size: 2215 1498 1255 692 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 4706 6418 Size: 274 80 33 51 1443 75 2654 1712 Cuts at: 6418 96 Size: 138 Fragments arranged by size: 2654 1712 1443 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2112 6420 6452 2080 Size: 32 4308 32 2088 Fragments arranged by size: 4308 2088 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6460 XbaI T'CTAG_A Cuts at: 3796 3796 Size: 6460 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6460 XmaI C'CCGG_G Cuts at: 1870 3785 1870 Size: 1915 4545 XmnI GAAnn'nnTTC Cuts at: 2369 4589 2369 Size: 2220 4240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4268 276 Size: 53 83 186 936 2734 2468 Fragments arranged by size: 2734 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI