(Linear) (Six-base) MAPSORT of: KIEE1363.seq Check: 3229 from: 1 to: 1755 KIEE1363.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1755 Size: 247 1508 AflIII A'CryG_T Cuts at: 0 352 1126 1755 Size: 352 774 629 Fragments arranged by size: 774 629 352 AgeI A'CCGG_T Cuts at: 0 1062 1755 Size: 1062 693 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 834 866 1755 Size: 834 32 889 Fragments arranged by size: 889 834 32 AlwNI CAG_nnn'CTG Cuts at: 0 1666 1731 1755 Size: 1666 65 24 Fragments arranged by size: 1666 65 24 ApaLI G'TGCA_C Cuts at: 0 578 1755 Size: 578 1177 ApoI r'AATT_y Cuts at: 0 244 843 1163 1700 1755 Size: 244 599 320 537 55 Fragments arranged by size: 599 537 320 244 55 AseI AT'TA_AT Cuts at: 0 509 1755 Size: 509 1246 BanI G'GyrC_C Cuts at: 0 3 1094 1527 1755 Size: 3 1091 433 228 Fragments arranged by size: 1091 433 228 3 BbeI G_GCGC'C Cuts at: 0 7 1755 Size: 7 1748 BciVI GTATCCnnnnn_n' Cuts at: 0 308 1150 1755 Size: 308 842 605 Fragments arranged by size: 842 605 308 Bme1580I G_kGCm'C Cuts at: 0 429 582 1099 1755 Size: 429 153 517 656 Fragments arranged by size: 656 517 429 153 BmrI ACTGGGnnnn_n' Cuts at: 0 356 1755 Size: 356 1399 BmtI G_CTAG'C Cuts at: 0 719 1755 Size: 719 1036 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1708 1755 Size: 1708 47 BsaHI Gr'CG_yC Cuts at: 0 4 1755 Size: 4 1751 BsaWI w'CCGG_w Cuts at: 0 1062 1755 Size: 1062 693 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 158 188 1116 1146 1755 Size: 158 30 928 30 609 Fragments arranged by size: 928 609 158 30 30 BseYI C'CCAG_C Cuts at: 0 310 1344 1755 Size: 310 1034 411 Fragments arranged by size: 1034 411 310 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1755 Size: 193 1562 BsiHKAI G_wGCw'C Cuts at: 0 582 1134 1552 1755 Size: 582 552 418 203 Fragments arranged by size: 582 552 418 203 Bsp1286I G_dGCh'C Cuts at: 0 429 582 1099 1134 1552 1755 Size: 429 153 517 35 418 203 Fragments arranged by size: 517 429 418 203 153 35 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1218 1755 Size: 1218 537 BsrBI CCG'CTC Cuts at: 0 18 1755 Size: 18 1737 BsrDI GCAATG_nn' Cuts at: 0 168 438 773 1755 Size: 168 270 335 982 Fragments arranged by size: 982 335 270 168 BsrFI r'CCGG_y Cuts at: 0 1062 1755 Size: 1062 693 BsrGI T'GTAC_A Cuts at: 0 47 1755 Size: 47 1708 EarI CTCTTCn'nnn_ Cuts at: 0 1382 1755 Size: 1382 373 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 1708 1755 Size: 247 1461 47 Fragments arranged by size: 1461 247 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 1723 1755 Size: 226 1497 32 Fragments arranged by size: 1497 226 32 EcoO109I rG'GnC_Cy Cuts at: 0 143 1587 1755 Size: 143 1444 168 Fragments arranged by size: 1444 168 143 EcoRI G'AATT_C Cuts at: 0 244 843 1755 Size: 244 599 912 Fragments arranged by size: 912 599 244 HaeII r_GCGC'y Cuts at: 0 7 1755 Size: 7 1748 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1192 1224 1755 Size: 1192 32 531 Fragments arranged by size: 1192 531 32 HincII GTy'rAC Cuts at: 0 438 729 1755 Size: 438 291 1026 Fragments arranged by size: 1026 438 291 HpaI GTT'AAC Cuts at: 0 438 1755 Size: 438 1317 KasI G'GCGC_C Cuts at: 0 3 1755 Size: 3 1752 MfeI C'AATT_G Cuts at: 0 479 1755 Size: 479 1276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1673 1755 Size: 1673 82 MslI CAynn'nnrTG Cuts at: 0 1196 1755 Size: 1196 559 MspA1I CmG'CkG Cuts at: 0 310 1755 Size: 310 1445 NarI GG'CG_CC Cuts at: 0 4 1755 Size: 4 1751 NheI G'CTAG_C Cuts at: 0 715 1755 Size: 715 1040 NspI r_CATG'y Cuts at: 0 356 1130 1755 Size: 356 774 625 Fragments arranged by size: 774 625 356 PciI A'CATG_T Cuts at: 0 352 1126 1755 Size: 352 774 629 Fragments arranged by size: 774 629 352 PflMI CCAn_nnn'nTGG Cuts at: 0 817 1731 1755 Size: 817 914 24 Fragments arranged by size: 914 817 24 PfoI T'CCnGG_A Cuts at: 0 564 1724 1755 Size: 564 1160 31 Fragments arranged by size: 1160 564 31 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 930 962 1755 Size: 930 32 793 Fragments arranged by size: 930 793 32 PpuMI rG'GwC_Cy Cuts at: 0 143 1587 1755 Size: 143 1444 168 Fragments arranged by size: 1444 168 143 PshAI GACnn'nnGTC Cuts at: 0 1514 1755 Size: 1514 241 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 749 781 1755 Size: 749 32 974 Fragments arranged by size: 974 749 32 PvuII CAG'CTG Cuts at: 0 310 1755 Size: 310 1445 SanDI GG'GwC_CC Cuts at: 0 1587 1755 Size: 1587 168 SfcI C'TryA_G Cuts at: 0 797 963 1755 Size: 797 166 792 Fragments arranged by size: 797 792 166 SfoI GGC'GCC Cuts at: 0 5 1755 Size: 5 1750 SspI AAT'ATT Cuts at: 0 180 1259 1755 Size: 180 1079 496 Fragments arranged by size: 1079 496 180 StyI C'CwwG_G Cuts at: 0 94 1755 Size: 94 1661 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 267 1755 Size: 267 1488 TatI w'GTAC_w Cuts at: 0 47 1755 Size: 47 1708 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 75 107 1755 Size: 75 32 1648 Fragments arranged by size: 1648 75 32 XmnI GAAnn'nnTTC Cuts at: 0 364 1755 Size: 364 1391 Enzymes that do cut: AcuI AflIII AgeI AloI AlwNI ApaLI ApoI AseI BanI BbeI BciVI Bme1580I BmrI BmtI BpmI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI KasI MfeI MmeI MslI MspA1I NarI NheI NspI PciI PflMI PfoI PpiI PpuMI PshAI PsrI PvuII SanDI SfcI SfoI SspI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AhdI AleI AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII KpnI MluI MscI NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PmeI PmlI PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI