################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI Hin1I HindIII HpaI HphI KasI Ksp632I KspI MaeI MamI MboII MfeI MluI MluNI MroI MscI MseI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 134 GGTACC G^GTACC Alw44I 532 GTGCAC G^TGCAC AlwI 475 GATCC GGATC(4/5) ApaLI 532 GTGCAC G^TGCAC Asp718I 134 GGTACC G^GTACC AspHI 532 GTGCAC G[AT]GC[AT]^C AviII 638 TGCGCA TGC^GCA BanI 134 GGTACC G^G[CT][GA]CC BglII 438 AGATCT A^GATCT BpmI 711 CTGGAG CTGGAG(16/14) BseRI 845 CTCCTC GAGGAG(10/8) BsiHKAI 532 GTGCAC G[AT]GC[AT]^C BsmFI 74 GGGAC GGGAC(10/14) BsrDI 302 CATTGC GCAATG(2/0) BsrI 71 ACTGG ACTGG(1/-1) BstEII 333 GGTAACC G^GT[ACGT]ACC BstPI 333 GGTAACC G^GT[ACGT]ACC BstXI 345 CCATGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 438 AGATCT [GA]^GATC[CT] EcoNI 231 CCTAAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 333 GGTAACC G^GT[ACGT]ACC EcoT14I 287 CCAAGG C^C[AT][AT]GG FseI 838 GGCCGGCC GGCCGG^CC FspI 638 TGCGCA TGC^GCA HaeII 216 GGCGCT [GA]GCGC^[CT] HgaI 918 GACGC GACGC(5/10) HincII 61 GTCAAC GT[CT]^[GA]AC HindII 61 GTCAAC GT[CT]^[GA]AC HinfI 108 GACTC G^A[ACGT]TC KpnI 134 GGTACC GGTAC^C MaeII 77 ACGT A^CGT MflI 438 AGATCT [GA]^GATC[CT] MslI 535 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PleI 108 GACTC GAGTC(4/5) StuI 755 AGGCCT AGG^CCT StyI 287 CCAAGG C^C[AT][AT]GG TaqI 395 TCGA T^CGA Tsp45I 283 GTGAC ^GT[GC]AC TthHB8I 395 TCGA T^CGA XcmI 287 CCAAGGCTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 438 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 600 GGACC G^G[AT]CC 741 GGACC BglI 757 GCCTTACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 834 GCCCGGCCGGC BsaWI 38 ACCGGA [AT]^CCGG[AT] 927 ACCGGA BsiEI 604 CGGCCG CG[GA][CT]^CG 837 CGGCCG BspMI 166 ACCTGC ACCTGC(4/8) 635 ACCTGC BsrBI 502 CCGCTC CCGCTC(-3/-3) 274 GAGCGG BssSI 442 CTCGTG CACGAG(-5/-1) 748 CTCGTG DdeI 869 CTGAG C^T[ACGT]AG 875 CTGAG DpnII 439 GATC ^GATC 475 GATC DsaI 557 CCGTGG C^C[GA][CT]GG 647 CCACGG EagI 604 CGGCCG C^GGCCG 837 CGGCCG EclXI 604 CGGCCG C^GGCCG 837 CGGCCG Eco52I 604 CGGCCG C^GGCCG 837 CGGCCG MaeIII 283 GTGAC ^GT[ACGT]AC 334 GTAAC MboI 439 GATC ^GATC 475 GATC NaeI 247 GCCGGC GCC^GGC 839 GCCGGC NdeII 439 GATC ^GATC 475 GATC NgoMIV 247 GCCGGC G^CCGGC 839 GCCGGC NlaIII 346 CATG CATG^ 387 CATG PflMI 189 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 552 CCACCCCGTGG Sau3AI 439 GATC ^GATC 475 GATC SfiI 756 GGCCTTACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 833 GGCCCGGCCGGCC Van91I 189 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 552 CCACCCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AvaI BanII BbvI BcnI BmyI BsaJI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DraIII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI TseI TspRI