(Circular) (Six-base) MAPSORT of: pFN21AE5306.seq Check: 1749 from: 1 to: 5641 pFN21AE5306 5641 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2180 2180 Size: 5641 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3451 278 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2984 1937 Size: 1047 4594 Acc65I G'GTAC_C Cuts at: 2139 2962 2139 Size: 823 4818 AclI AA'CG_TT Cuts at: 1563 3768 4141 1563 Size: 2205 373 3063 Fragments arranged by size: 3063 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 3704 5088 5356 1632 Size: 890 1182 1384 268 1917 Fragments arranged by size: 1917 1384 1182 890 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5444 AflIII A'CryG_T Cuts at: 1203 4540 5517 1203 Size: 3337 977 1327 Fragments arranged by size: 3337 1327 977 AgeI A'CCGG_T Cuts at: 4472 4472 Size: 5641 AhdI GACnn_n'nnGTC Cuts at: 4370 4370 Size: 5641 AleI CACnn'nnGTG Cuts at: 1166 2544 1166 Size: 1378 4263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2562 1184 Size: 1378 4263 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2597 2629 2597 Size: 32 5609 AlwNI CAG_nnn'CTG Cuts at: 2190 2743 2911 3384 4956 2190 Size: 553 168 473 1572 2875 Fragments arranged by size: 2875 1572 553 473 168 ApaI G_GGCC'C Cuts at: 2386 2804 2841 2897 2386 Size: 418 37 56 5130 Fragments arranged by size: 5130 418 56 37 ApaLI G'TGCA_C Cuts at: 2537 3698 4854 2537 Size: 1161 1156 3324 Fragments arranged by size: 3324 1161 1156 ApoI r'AATT_y Cuts at: 1493 2950 3176 3328 5437 1493 Size: 1457 226 152 2109 1697 Fragments arranged by size: 2109 1697 1457 226 152 AseI AT'TA_AT Cuts at: 160 1032 4195 160 Size: 872 3163 1606 Fragments arranged by size: 3163 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2593 2789 2966 1870 Size: 73 207 443 196 177 4545 Fragments arranged by size: 4545 443 207 196 177 73 BamHI G'GATC_C Cuts at: 2971 3338 2971 Size: 367 5274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2139 2962 4417 618 Size: 325 217 627 352 823 1455 1842 Fragments arranged by size: 1842 1455 823 627 352 325 217 BanII G_rGCy'C Cuts at: 1391 2386 2408 2723 2804 2841 2897 2960 1391 Size: 995 22 315 81 37 56 63 4072 Fragments arranged by size: 4072 995 315 81 63 56 37 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3832 3866 1903 Size: 34 1895 34 3678 Fragments arranged by size: 3678 1895 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3533 4743 3533 Size: 1210 4431 BclI T'GATC_A Cuts at: 1548 1548 Size: 5641 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2768 2845 4252 Size: 107 122 71 631 605 1096 77 1407 Cuts at: 4252 136 Size: 1525 Fragments arranged by size: 1525 1407 1096 631 605 122 107 77 71 BglII A'GATC_T Cuts at: 2443 5636 2443 Size: 3193 2448 BlpI GC'TnA_GC Cuts at: 3058 3058 Size: 5641 Bme1580I G_kGCm'C Cuts at: 1790 2322 2386 2541 2551 2804 2841 2897 3702 Size: 532 64 155 10 253 37 56 805 Cuts at: 3702 4858 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 805 532 253 155 64 56 37 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2086 3372 4330 Size: 416 53 83 399 24 669 1286 958 Cuts at: 4330 442 Size: 1753 Fragments arranged by size: 1753 1286 958 669 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5641 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2737 4301 1239 Size: 1498 1564 2579 Fragments arranged by size: 2579 1564 1498 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3766 4631 4929 3766 Size: 865 298 4478 Fragments arranged by size: 4478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4304 882 Size: 617 2805 2219 Fragments arranged by size: 2805 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3432 493 Size: 711 2228 2702 Fragments arranged by size: 2702 2228 711 BsaBI GATnn'nnATC Cuts at: 5635 5635 Size: 5641 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3448 3830 4465 275 Size: 53 83 186 936 1915 382 635 1451 Fragments arranged by size: 1915 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2043 2932 4074 4472 4746 4893 5304 2043 Size: 889 1142 398 274 147 411 2380 Fragments arranged by size: 2380 1142 889 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2015 2045 1178 Size: 30 165 30 612 30 4774 Fragments arranged by size: 4774 612 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2841 1186 Size: 1655 3986 BseYI C'CCAG_C Cuts at: 4844 5340 4844 Size: 496 5145 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 2662 2777 1163 Size: 573 876 50 115 4027 Fragments arranged by size: 4027 876 573 115 50 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2612 2845 3091 3852 4880 1540 Size: 275 186 611 233 246 761 1028 2301 Fragments arranged by size: 2301 1028 761 611 275 246 233 186 BsiHKAI G_wGCw'C Cuts at: 2541 3702 3787 4858 2541 Size: 1161 85 1071 3324 Fragments arranged by size: 3324 1161 1071 85 BsmI GAATG_Cn' Cuts at: 3152 3245 3152 Size: 93 5548 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2386 2408 2541 2551 2723 2804 Size: 399 532 64 22 133 10 172 81 Cuts at: 2804 2841 2897 2960 3702 3787 4858 1391 Size: 37 56 63 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 399 172 133 85 81 64 63 56 37 22 10 BspEI T'CCGG_A Cuts at: 5304 5304 Size: 5641 BspHI T'CATG_A Cuts at: 3530 5260 3530 Size: 1730 3911 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2180 2649 2996 844 Size: 1336 469 347 3489 Fragments arranged by size: 3489 1336 469 347 BsrBI CCG'CTC Cuts at: 1394 1823 2281 2509 3528 1394 Size: 429 458 228 1019 3507 Fragments arranged by size: 3507 1019 458 429 228 BsrDI GCAATG_nn' Cuts at: 65 1248 2306 4136 4310 65 Size: 1183 1058 1830 174 1396 Fragments arranged by size: 1830 1396 1183 1058 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2252 2607 2844 4285 4472 1700 Size: 110 442 355 237 1441 187 2869 Fragments arranged by size: 2869 1441 442 355 237 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5641 BssSI C'ACGA_G Cuts at: 2447 2753 3701 4713 2447 Size: 306 948 1012 3375 Fragments arranged by size: 3375 1012 948 306 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5641 BstBI TT'CG_AA Cuts at: 4445 4445 Size: 5641 BstEII G'GTnAC_C Cuts at: 1184 2338 1184 Size: 1154 4487 BstXI CCAn_nnnn'nTGG Cuts at: 2357 2357 Size: 5641 BstYI r'GATC_y Cuts at: 1972 2443 2971 3338 3723 3740 5192 5278 5290 Size: 471 528 367 385 17 1452 86 12 Cuts at: 5290 5636 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 528 471 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2562 2652 513 Size: 552 939 558 90 3502 Fragments arranged by size: 3502 939 558 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4902 Fragments arranged by size: 4902 683 56 BtsI GCAGTG_nn' Cuts at: 2539 2551 2729 2912 3157 3951 3971 2539 Size: 12 178 183 245 794 20 4209 Fragments arranged by size: 4209 794 245 183 178 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5641 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5606 DraI TTT'AAA Cuts at: 2945 2945 Size: 5641 DraIII CAC_nnn'GTG Cuts at: 2394 2563 2664 2394 Size: 169 101 5371 Fragments arranged by size: 5371 169 101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4648 809 Size: 507 3332 1802 Fragments arranged by size: 3332 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2609 2842 Size: 54 1425 50 275 18 327 452 233 Cuts at: 2842 3088 3977 5606 8 Size: 246 889 1629 43 Fragments arranged by size: 1629 1425 889 452 327 275 246 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2609 2842 3088 1537 Size: 275 797 233 246 4090 Fragments arranged by size: 4090 797 275 246 233 EarI CTCTTCn'nnn_ Cuts at: 1353 3571 1353 Size: 2218 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4228 4602 4748 387 Size: 1064 138 2639 374 146 1280 Fragments arranged by size: 2639 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2737 3704 4301 5088 5356 1239 Size: 393 890 215 967 597 787 268 1524 Fragments arranged by size: 1524 967 890 787 597 393 268 215 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 5641 EcoO109I rG'GnC_Cy Cuts at: 2800 2801 2893 2894 2800 Size: 1 92 1 5547 Fragments arranged by size: 5547 92 1 1 EcoRI G'AATT_C Cuts at: 2950 3328 2950 Size: 378 5263 FseI GG_CCGG'CC Cuts at: 2848 2848 Size: 5641 FspI TGC'GCA Cuts at: 2645 4147 2645 Size: 1502 4139 HaeII r_GCGC'y Cuts at: 1678 2225 4788 1678 Size: 547 2563 2531 Fragments arranged by size: 2563 2531 547 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 2471 2503 4282 Size: 32 135 32 443 32 424 32 1779 Cuts at: 4282 4314 4356 4388 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1779 443 424 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 2985 3231 669 Size: 988 281 130 917 246 3079 Fragments arranged by size: 3079 988 917 281 246 130 HindIII A'AGCT_T Cuts at: 748 748 Size: 5641 HpaI GTT'AAC Cuts at: 3231 3231 Size: 5641 KpnI G_GTAC'C Cuts at: 2143 2966 2143 Size: 823 4818 MfeI C'AATT_G Cuts at: 3240 3240 Size: 5641 MluI A'CGCG_T Cuts at: 5517 5517 Size: 5641 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2895 4755 4939 1305 Size: 1590 1860 184 2007 Fragments arranged by size: 2007 1860 1590 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3819 Fragments arranged by size: 3819 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 3599 3958 4117 518 Size: 648 1378 1055 359 159 2042 Fragments arranged by size: 2042 1378 1055 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2200 2217 2530 2890 3057 3734 4882 Size: 528 66 17 313 360 167 677 1148 Cuts at: 4882 5127 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 528 360 313 245 167 66 17 NaeI GCC'GGC Cuts at: 2254 2846 2254 Size: 592 5049 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4150 Fragments arranged by size: 4150 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5641 NgoMIV G'CCGG_C Cuts at: 2252 2844 2252 Size: 592 5049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5641 NotI GC'GGCC_GC Cuts at: 3088 3088 Size: 5641 NspI r_CATG'y Cuts at: 1132 1229 2999 4544 1132 Size: 97 1770 1545 2229 Fragments arranged by size: 2229 1770 1545 97 PasI CC'CwG_GG Cuts at: 1348 2551 1348 Size: 1203 4438 PciI A'CATG_T Cuts at: 4540 4540 Size: 5641 PflMI CCAn_nnn'nTGG Cuts at: 1108 2200 2563 1108 Size: 1092 363 4186 Fragments arranged by size: 4186 1092 363 PfoI T'CCnGG_A Cuts at: 2325 2325 Size: 5641 PmeI GTTT'AAAC Cuts at: 2945 2945 Size: 5641 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3710 3742 5223 5255 3710 Size: 32 1481 32 4096 Fragments arranged by size: 4096 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5641 PsiI TTA'TAA Cuts at: 3211 3211 Size: 5641 PspOMI G'GGCC_C Cuts at: 2382 2800 2837 2893 2382 Size: 418 37 56 5130 Fragments arranged by size: 5130 418 56 37 PstI C_TGCA'G Cuts at: 830 2127 2546 2739 2907 2993 830 Size: 1297 419 193 168 86 3478 Fragments arranged by size: 3478 1297 419 193 168 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5641 PvuII CAG'CTG Cuts at: 2134 2200 2530 2134 Size: 66 330 5245 Fragments arranged by size: 5245 330 66 SalI G'TCGA_C Cuts at: 1936 2983 1936 Size: 1047 4594 SbfI CC_TGCA'GG Cuts at: 2993 2993 Size: 5641 ScaI AGT'ACT Cuts at: 3889 5601 3889 Size: 1712 3929 SfcI C'TryA_G Cuts at: 826 1045 2123 2542 2735 2903 2989 4124 4805 Size: 219 1078 419 193 168 86 1135 681 Cuts at: 4805 4996 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1078 681 419 219 193 191 168 86 SfiI GGCCn_nnn'nGGCC Cuts at: 2768 2845 2768 Size: 77 5564 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5641 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5641 SmaI CCC'GGG Cuts at: 1872 2968 1872 Size: 1096 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3745 4646 4908 820 Size: 197 926 1802 901 262 1553 Fragments arranged by size: 1802 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3432 493 Size: 2939 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5641 SphI G_CATG'C Cuts at: 1132 2999 1132 Size: 1867 3774 SspI AAT'ATT Cuts at: 5 52 3565 5 Size: 47 3513 2081 Fragments arranged by size: 3513 2081 47 StuI AGG'CCT Cuts at: 1517 2762 1517 Size: 1245 4396 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 3080 513 Size: 552 939 288 788 3074 Fragments arranged by size: 3074 939 788 552 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3668 3685 3838 4023 4468 325 Size: 1255 2088 17 153 185 445 1498 Fragments arranged by size: 2088 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3887 5599 96 Size: 274 80 33 51 1443 1910 1712 138 Fragments arranged by size: 1910 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2648 2680 5601 5633 2648 Size: 32 2921 32 2656 Fragments arranged by size: 2921 2656 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5641 XbaI T'CTAG_A Cuts at: 2977 2977 Size: 5641 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2299 2299 Size: 5641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5641 XmaI C'CCGG_G Cuts at: 1870 2966 1870 Size: 1096 4545 XmnI GAAnn'nnTTC Cuts at: 3770 3770 Size: 5641 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3449 276 Size: 53 83 186 936 1915 2468 Fragments arranged by size: 2468 1915 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI