(Linear) (Six-base) MAPSORT of: KIEE5306.seq Check: 1419 from: 1 to: 936 KIEE5306.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 175 936 Size: 175 761 Acc65I G'GTAC_C Cuts at: 0 134 936 Size: 134 802 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 936 Size: 517 419 AleI CACnn'nnGTG Cuts at: 0 539 936 Size: 539 397 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 557 936 Size: 557 379 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 592 624 936 Size: 592 32 312 Fragments arranged by size: 592 312 32 AlwNI CAG_nnn'CTG Cuts at: 0 185 738 906 936 Size: 185 553 168 30 Fragments arranged by size: 553 185 168 30 ApaI G_GGCC'C Cuts at: 0 381 799 836 892 936 Size: 381 418 37 56 44 Fragments arranged by size: 418 381 56 44 37 ApaLI G'TGCA_C Cuts at: 0 532 936 Size: 532 404 AvaI C'yCGr_G Cuts at: 0 145 588 784 936 Size: 145 443 196 152 Fragments arranged by size: 443 196 152 145 BanI G'GyrC_C Cuts at: 0 134 936 Size: 134 802 BanII G_rGCy'C Cuts at: 0 381 403 718 799 836 892 936 Size: 381 22 315 81 37 56 44 Fragments arranged by size: 381 315 81 56 44 37 22 BglI GCCn_nnn'nGGC Cuts at: 0 763 840 936 Size: 763 77 96 Fragments arranged by size: 763 96 77 BglII A'GATC_T Cuts at: 0 438 936 Size: 438 498 Bme1580I G_kGCm'C Cuts at: 0 317 381 536 546 799 836 892 936 Size: 317 64 155 10 253 37 56 44 Fragments arranged by size: 317 253 155 64 56 44 37 10 BmrI ACTGGGnnnn_n' Cuts at: 0 81 936 Size: 81 855 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 732 936 Size: 732 204 BsaWI w'CCGG_w Cuts at: 0 38 927 936 Size: 38 889 9 Fragments arranged by size: 889 38 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 10 40 936 Size: 10 30 896 Fragments arranged by size: 896 30 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 836 936 Size: 836 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 657 772 936 Size: 607 50 115 164 Fragments arranged by size: 607 164 115 50 BsiEI CG_ry'CG Cuts at: 0 607 840 936 Size: 607 233 96 Fragments arranged by size: 607 233 96 BsiHKAI G_wGCw'C Cuts at: 0 536 936 Size: 536 400 Bsp1286I G_dGCh'C Cuts at: 0 317 381 403 536 546 718 799 836 Size: 317 64 22 133 10 172 81 37 Cuts at: 836 892 936 Size: 56 44 Fragments arranged by size: 317 172 133 81 64 56 44 37 22 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 175 644 936 Size: 175 469 292 Fragments arranged by size: 469 292 175 BsrBI CCG'CTC Cuts at: 0 276 504 936 Size: 276 228 432 Fragments arranged by size: 432 276 228 BsrDI GCAATG_nn' Cuts at: 0 301 936 Size: 301 635 BsrFI r'CCGG_y Cuts at: 0 247 602 839 936 Size: 247 355 237 97 Fragments arranged by size: 355 247 237 97 BssSI C'ACGA_G Cuts at: 0 442 748 936 Size: 442 306 188 Fragments arranged by size: 442 306 188 BstEII G'GTnAC_C Cuts at: 0 333 936 Size: 333 603 BstXI CCAn_nnnn'nTGG Cuts at: 0 352 936 Size: 352 584 BstYI r'GATC_y Cuts at: 0 438 936 Size: 438 498 BtgI C'CryG_G Cuts at: 0 557 647 936 Size: 557 90 289 Fragments arranged by size: 557 289 90 BtsI GCAGTG_nn' Cuts at: 0 534 546 724 907 936 Size: 534 12 178 183 29 Fragments arranged by size: 534 183 178 29 12 DraIII CAC_nnn'GTG Cuts at: 0 389 558 659 936 Size: 389 169 101 277 Fragments arranged by size: 389 277 169 101 EaeI y'GGCC_r Cuts at: 0 152 604 837 936 Size: 152 452 233 99 Fragments arranged by size: 452 233 152 99 EagI C'GGCC_G Cuts at: 0 604 837 936 Size: 604 233 99 Fragments arranged by size: 604 233 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 732 936 Size: 517 215 204 Fragments arranged by size: 517 215 204 EcoNI CCTnn'n_nnAGG Cuts at: 0 235 936 Size: 235 701 EcoO109I rG'GnC_Cy Cuts at: 0 795 796 888 889 936 Size: 795 1 92 1 47 Fragments arranged by size: 795 92 47 1 1 FseI GG_CCGG'CC Cuts at: 0 843 936 Size: 843 93 FspI TGC'GCA Cuts at: 0 640 936 Size: 640 296 HaeII r_GCGC'y Cuts at: 0 220 936 Size: 220 716 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 10 42 466 498 936 Size: 10 32 424 32 438 Fragments arranged by size: 438 424 32 32 10 HincII GTy'rAC Cuts at: 0 63 936 Size: 63 873 KpnI G_GTAC'C Cuts at: 0 138 936 Size: 138 798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 890 936 Size: 890 46 MslI CAynn'nnrTG Cuts at: 0 539 936 Size: 539 397 MspA1I CmG'CkG Cuts at: 0 129 195 212 525 885 936 Size: 129 66 17 313 360 51 Fragments arranged by size: 360 313 129 66 51 17 NaeI GCC'GGC Cuts at: 0 249 841 936 Size: 249 592 95 Fragments arranged by size: 592 249 95 NgoMIV G'CCGG_C Cuts at: 0 247 839 936 Size: 247 592 97 Fragments arranged by size: 592 247 97 PasI CC'CwG_GG Cuts at: 0 546 936 Size: 546 390 PflMI CCAn_nnn'nTGG Cuts at: 0 195 558 936 Size: 195 363 378 Fragments arranged by size: 378 363 195 PfoI T'CCnGG_A Cuts at: 0 320 936 Size: 320 616 PspOMI G'GGCC_C Cuts at: 0 377 795 832 888 936 Size: 377 418 37 56 48 Fragments arranged by size: 418 377 56 48 37 PstI C_TGCA'G Cuts at: 0 122 541 734 902 936 Size: 122 419 193 168 34 Fragments arranged by size: 419 193 168 122 34 PvuII CAG'CTG Cuts at: 0 129 195 525 936 Size: 129 66 330 411 Fragments arranged by size: 411 330 129 66 SfcI C'TryA_G Cuts at: 0 118 537 730 898 936 Size: 118 419 193 168 38 Fragments arranged by size: 419 193 168 118 38 SfiI GGCCn_nnn'nGGCC Cuts at: 0 763 840 936 Size: 763 77 96 Fragments arranged by size: 763 96 77 StuI AGG'CCT Cuts at: 0 757 936 Size: 757 179 StyI C'CwwG_G Cuts at: 0 287 936 Size: 287 649 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 643 675 936 Size: 643 32 261 Fragments arranged by size: 643 261 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 294 936 Size: 294 642 Enzymes that do cut: AarI Acc65I AcuI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BanI BanII BglI BglII Bme1580I BmrI BpmI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EagI Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII KpnI MmeI MslI MspA1I NaeI NgoMIV PasI PflMI PfoI PspOMI PstI PvuII SfcI SfiI StuI StyI TstI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseYI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FspAI HindIII HpaI KasI MfeI MluI MscI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI