################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I MamI MboII MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp509I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 703 GTAGAC GT^[AC][GT]AC AlwI 126 GATCC GGATC(4/5) Aor51HI 754 AGCGCT AGC^GCT AspI 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 759 TGGCCA TGG^CCA BciVI 102 GGATAC GTATCC(6/5) BfaI 361 CTAG C^TAG BglII 81 AGATCT A^GATCT BlpI 574 GCTGAGC GC^T[ACGT]AGC Bpu1102I 574 GCTGAGC GC^T[ACGT]AGC BsaWI 116 TCCGGT [AT]^CCGG[AT] BsmFI 532 GGGAC GGGAC(10/14) BspHI 626 TCATGA T^CATGA BsrDI 495 CATTGC GCAATG(2/0) BsrFI 250 GCCGGC [GA]^CCGG[CT] BssHII 153 GCGCGC G^CGCGC BstEII 582 GGTGACC G^GT[ACGT]ACC BstPI 582 GGTGACC G^GT[ACGT]ACC BstXI 407 CCACGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 574 GCTGAGC GC^T[ACGT]AGC Cfr10I 250 GCCGGC [GA]^CCGG[CT] EaeI 759 TGGCCA [CT]^GGCC[GA] Eco47III 754 AGCGCT AGC^GCT EcoNI 594 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 582 GGTGACC G^GT[ACGT]ACC HincII 565 GTCAAC GT[CT]^[GA]AC HindII 565 GTCAAC GT[CT]^[GA]AC KspI 177 CCGCGG CCGC^GG MaeI 361 CTAG C^TAG MluNI 759 TGGCCA TGG^CCA MscI 759 TGGCCA TGG^CCA NaeI 250 GCCGGC GCC^GGC NcoI 203 CCATGG C^CATGG NgoMIV 250 GCCGGC G^CCGGC NspI 336 GCATGC [GA]CATG^[CT] PflFI 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 532 GGGACCT [GA]G^G[AT]CC[CT] PstI 256 CTGCAG CTGCA^G RcaI 626 TCATGA T^CATGA SacII 177 CCGCGG CCGC^GG SmaI 443 CCCGGG CCC^GGG SphI 336 GCATGC GCATG^C Tth111I 706 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 443 CCCGGG C^CCGGG XmaI 443 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 154 CGCG CG^CG 178 CGCG ApaI 219 GGGCCC GGGCC^C 793 GGGCCC AvaII 423 GGTCC G^G[AT]CC 533 GGACC BanI 190 GGCACC G^G[CT][GA]CC 379 GGTGCC BglI 661 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 889 GCCCTCATGGC BpmI 781 CTCCAG CTGGAG(16/14) 449 CTGGAG BsgI 678 CTGCAC GTGCAG(16/14) 903 CTGCAC BstF5I 411 GGATG GGATG(2/0) 603 GGATG BstUI 154 CGCG CG^CG 178 CGCG BstYI 81 AGATCT [GA]^GATC[CT] 125 AGATCC DpnII 82 GATC ^GATC 126 GATC FokI 411 GGATG GGATG(9/13) 603 GGATG HinfI 301 GATTC G^A[ACGT]TC 428 GATTC MaeII 707 ACGT A^CGT 851 ACGT MboI 82 GATC ^GATC 126 GATC MflI 81 AGATCT [GA]^GATC[CT] 125 AGATCC MslI 18 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 408 CACGGATGTG MvnI 154 CGCG CG^CG 178 CGCG NdeII 82 GATC ^GATC 126 GATC PvuII 65 CAGCTG CAG^CTG 877 CAGCTG Sau3AI 82 GATC ^GATC 126 GATC TfiI 301 GATTC G^A[AT]TC 428 GATTC Tsp45I 583 GTGAC ^GT[GC]AC 865 GTCAC XcmI 194 CCATTCTGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 374 CCACAGGTGCCCTGG XhoII 81 AGATCT [GA]^GATC[CT] 125 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AvaI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI