(Circular) (Six-base) MAPSORT of: pFN21AE1315.seq Check: 3093 from: 1 to: 5632 pFN21AE1315 5632 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 2975 1937 Size: 772 266 4594 Fragments arranged by size: 4594 772 266 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3695 5079 5347 1632 Size: 2063 1384 268 1917 Fragments arranged by size: 2063 1917 1384 268 AfeI AGC'GCT Cuts at: 2761 2761 Size: 5632 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 2027 2417 1166 Size: 861 390 4381 Fragments arranged by size: 4381 861 390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2698 1184 Size: 1514 4118 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaI G_GGCC'C Cuts at: 2228 2802 2228 Size: 574 5058 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2941 3167 3319 5428 1493 Size: 1448 226 152 2109 1697 Fragments arranged by size: 2109 1697 1448 226 152 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2448 2505 2802 2916 2957 1870 Size: 73 505 57 297 114 41 4545 Fragments arranged by size: 4545 505 297 114 73 57 41 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2195 2384 2953 4408 618 Size: 325 217 627 408 189 569 1455 1842 Fragments arranged by size: 1842 1455 627 569 408 325 217 189 BanII G_rGCy'C Cuts at: 1391 2228 2336 2802 2896 2951 1391 Size: 837 108 466 94 55 4072 Fragments arranged by size: 4072 837 466 108 94 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4661 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2101 3524 4734 2101 Size: 1423 1210 2999 Fragments arranged by size: 2999 1423 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2672 2900 4243 Size: 107 122 71 631 605 1000 228 1343 Cuts at: 4243 136 Size: 1525 Fragments arranged by size: 1525 1343 1000 631 605 228 122 107 71 BglII A'GATC_T Cuts at: 2086 5627 2086 Size: 3541 2091 BlpI GC'TnA_GC Cuts at: 2580 3049 2580 Size: 469 5163 Bme1580I G_kGCm'C Cuts at: 1790 2034 2198 2228 2389 2776 2802 3693 4849 Size: 244 164 30 161 387 26 891 1156 Cuts at: 4849 1790 Size: 2573 Fragments arranged by size: 2573 1156 891 387 244 164 161 30 26 BmgBI CAC'GTC Cuts at: 1318 2713 1318 Size: 1395 4237 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2170 3363 4321 Size: 416 53 83 399 24 753 1193 958 Cuts at: 4321 442 Size: 1753 Fragments arranged by size: 1753 1193 958 753 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 2771 4292 1239 Size: 1236 296 1521 2579 Fragments arranged by size: 2579 1521 1236 296 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3757 4622 4920 3757 Size: 865 298 4469 Fragments arranged by size: 4469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2121 4065 4463 4737 4884 5295 2121 Size: 1944 398 274 147 411 2458 Fragments arranged by size: 2458 1944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 2406 4835 5331 2406 Size: 2429 496 2707 Fragments arranged by size: 2707 2429 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2668 2893 1163 Size: 573 932 225 3902 Fragments arranged by size: 3902 932 573 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3693 3778 4849 3693 Size: 85 1071 4476 Fragments arranged by size: 4476 1071 85 BsmI GAATG_Cn' Cuts at: 3143 3236 3143 Size: 93 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2198 2228 2336 2389 2776 2802 Size: 399 244 164 30 108 53 387 26 Cuts at: 2802 2896 2951 3693 3778 4849 1391 Size: 94 55 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 244 164 108 94 85 55 53 30 26 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2631 3521 5251 2631 Size: 890 1730 3012 Fragments arranged by size: 3012 1730 890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2499 4127 4301 65 Size: 1183 1251 1628 174 1396 Fragments arranged by size: 1628 1396 1251 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 4276 4463 1700 Size: 110 445 2021 187 2869 Fragments arranged by size: 2869 2021 445 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 2158 1925 Size: 233 5399 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 2587 1184 Size: 1403 4229 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2086 2130 2962 3329 3714 3731 5183 5269 Size: 114 44 832 367 385 17 1452 86 Cuts at: 5269 5281 5627 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 832 385 367 346 114 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2182 2208 2412 513 Size: 552 939 178 26 204 3733 Fragments arranged by size: 3733 939 552 204 178 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2065 2680 487 Size: 683 56 839 615 3439 Fragments arranged by size: 3439 839 683 615 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3079 3968 Size: 54 1425 50 275 18 934 315 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 934 889 315 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3562 1353 Size: 2209 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 2771 3695 4292 5079 5347 1239 Size: 393 843 296 924 597 787 268 1524 Fragments arranged by size: 1524 924 843 787 597 393 296 268 EcoNI CCTnn'n_nnAGG Cuts at: 2603 2603 Size: 5632 EcoO109I rG'GnC_Cy Cuts at: 2224 2225 2524 2538 2798 2799 2224 Size: 1 299 14 260 1 5057 Fragments arranged by size: 5057 299 260 14 1 1 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2175 2269 2763 4779 1678 Size: 497 94 494 2016 2531 Fragments arranged by size: 2531 2016 497 494 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2572 2976 3222 669 Size: 988 281 634 404 246 3079 Fragments arranged by size: 3079 988 634 404 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2444 4746 4930 1305 Size: 1139 2302 184 2007 Fragments arranged by size: 2302 2007 1139 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2766 10 Size: 54 1425 343 934 2876 Fragments arranged by size: 2876 1425 934 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2417 3590 3949 4108 518 Size: 648 861 390 1173 359 159 2042 Fragments arranged by size: 2042 1173 861 648 390 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2184 2884 3048 3725 4873 5118 1606 Size: 466 112 700 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 700 677 466 245 164 112 NaeI GCC'GGC Cuts at: 2257 2257 Size: 5632 NcoI C'CATG_G Cuts at: 513 1065 2004 2208 513 Size: 552 939 204 3937 Fragments arranged by size: 3937 939 552 204 NdeI CA'TA_TG Cuts at: 387 387 Size: 5632 NgoMIV G'CCGG_C Cuts at: 2255 2255 Size: 5632 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2345 2990 4535 1132 Size: 97 1116 645 1545 2229 Fragments arranged by size: 2229 1545 1116 645 97 PasI CC'CwG_GG Cuts at: 1348 2743 1348 Size: 1395 4237 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PfoI T'CCnGG_A Cuts at: 2849 2849 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2501 2533 3701 3733 5214 5246 2501 Size: 32 1168 32 1481 32 2887 Fragments arranged by size: 2887 1481 1168 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2538 2538 Size: 5632 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PspOMI G'GGCC_C Cuts at: 2224 2798 2224 Size: 574 5058 PstI C_TGCA'G Cuts at: 830 2265 2984 830 Size: 1435 719 3478 Fragments arranged by size: 3478 1435 719 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2072 2884 2072 Size: 812 4820 SacII CC_GC'GG Cuts at: 2185 2185 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2163 2261 2706 2980 4115 4796 4987 Size: 219 1118 98 445 274 1135 681 191 Cuts at: 4987 826 Size: 1471 Fragments arranged by size: 1471 1135 1118 681 445 274 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2450 2959 1872 Size: 578 509 4545 Fragments arranged by size: 4545 578 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 3736 4637 4899 820 Size: 197 926 1793 901 262 1553 Fragments arranged by size: 1793 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2345 2990 1132 Size: 1213 645 3774 Fragments arranged by size: 3774 1213 645 SrfI GCCC'GGGC Cuts at: 2450 2450 Size: 5632 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2134 2208 2328 3071 513 Size: 552 939 130 74 120 743 3074 Fragments arranged by size: 3074 939 743 552 130 120 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2751 3878 5590 Size: 274 80 33 51 1443 774 1127 1712 Cuts at: 5590 96 Size: 138 Fragments arranged by size: 1712 1443 1127 774 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 2714 1316 Size: 1398 4234 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2206 2386 2206 Size: 180 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2448 2957 1870 Size: 578 509 4545 Fragments arranged by size: 4545 578 509 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SwaI