################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvrII BbeI BbsI BbvCI BciVI BfrI BglII BlnI BpuAI BsaBI BsaHI BseAI BseRI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI HaeII Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 149 ACACGT A^C[GA][CT]GT Alw44I 384 GTGCAC G^TGCAC ApaI 737 GGGCCC GGGCC^C ApaLI 384 GTGCAC G^TGCAC AspHI 384 GTGCAC G[AT]GC[AT]^C AspI 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 233 TGCGCA TGC^GCA BamHI 362 GGATCC G^GATCC BanI 1037 GGTGCC G^G[CT][GA]CC BcgI 332 GCAAGGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 275 TGATCA T^GATCA BglI 832 GCCGAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1131 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1131 GCTGAGC GC^T[ACGT]AGC BsaWI 359 ACCGGA [AT]^CCGG[AT] BsgI 1111 GTGCAG GTGCAG(16/14) BsiEI 536 CGGCCG CG[GA][CT]^CG BsiHKAI 384 GTGCAC G[AT]GC[AT]^C Bsp1407I 953 TGTACA T^GTACA BsrBI 463 GAGCGG CCGCTC(-3/-3) BsrDI 50 GCAATG GCAATG(2/0) BsrGI 953 TGTACA T^GTACA BssSI 847 CACGAG CACGAG(-5/-1) BstAPI 50 GCAATGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1131 GCTGAGC GC^T[ACGT]AGC EagI 536 CGGCCG C^GGCCG EclXI 536 CGGCCG C^GGCCG Eco52I 536 CGGCCG C^GGCCG EcoNI 1101 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1106 CCAAGG C^C[AT][AT]GG FbaI 275 TGATCA T^GATCA FspI 233 TGCGCA TGC^GCA HgaI 660 GCGTC GACGC(5/10) MaeIII 914 GTGAC ^GT[ACGT]AC MslI 534 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 633 CATATG CA^TATG PflFI 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 631 GACATATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 394 CTGCAG CTGCA^G SapI 255 GCTCTTC GCTCTTC(1/4) SexAI 347 ACCTGGT A^CC[AT]GGT SphI 1127 GCATGC GCATG^C SspBI 953 TGTACA T^GTACA StyI 1106 CCAAGG C^C[AT][AT]GG TfiI 1216 GATTC G^A[AT]TC Tsp45I 914 GTGAC ^GT[GC]AC Tth111I 346 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 533 CCACGGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 343 GTCGAC GT^[AC][GT]AC 610 GTCGAC AccII 987 CGCG CG^CG 1152 CGCG BalI 806 TGGCCA TGG^CCA 1103 TGGCCA BbrPI 22 CACGTG CAC^GTG 181 CACGTG BcnI 281 CCGGG CC^[GC]GG 819 CCCGG BfaI 499 CTAG C^TAG 775 CTAG BsaI 1097 GAGACC GGTCTC(1/5) 528 GGTCTC BstUI 987 CGCG CG^CG 1152 CGCG BstYI 362 GGATCC [GA]^GATC[CT] 378 GGATCT DraII 736 GGGGCCC [GA]G^G[ACGT]CC[CT] 737 GGGCCCT EarI 256 CTCTTC CTCTTC(1/4) 1201 GAAGAG EcoO109I 736 GGGGCCC [GA]G^G[ACGT]CC[CT] 737 GGGCCCT EcoRI 86 GAATTC G^AATTC 760 GAATTC FauI 788 CCCGC CCCGC(4/6) 1116 GCGGG Ksp632I 256 CTCTTC CTCTTC(1/4) 1201 GAAGAG MaeI 499 CTAG C^TAG 775 CTAG MflI 362 GGATCC [GA]^GATC[CT] 378 GGATCT MluNI 806 TGGCCA TGG^CCA 1103 TGGCCA MscI 806 TGGCCA TGG^CCA 1103 TGGCCA MseI 1052 TTAA T^TAA 1064 TTAA MvnI 987 CGCG CG^CG 1152 CGCG NciI 281 CCGGG CC^[GC]GG 819 CCCGG NspI 947 GCATGT [GA]CATG^[CT] 1127 GCATGC PleI 139 GACTC GAGTC(4/5) 38 GAGTC PmaCI 22 CACGTG CAC^GTG 181 CACGTG PmlI 22 CACGTG CAC^GTG 181 CACGTG PvuII 271 CAGCTG CAG^CTG 853 CAGCTG SalI 343 GTCGAC G^TCGAC 610 GTCGAC SfcI 246 CTATAG C^T[GA][CT]AG 394 CTGCAG StuI 839 AGGCCT AGG^CCT 1157 AGGCCT Tru9I 1052 TTAA T^TAA 1064 TTAA XhoII 362 GGATCC [GA]^GATC[CT] 378 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BanII BbvI BmyI BpmI BsaAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I