(Circular) (Six-base) MAPSORT of: pFN21AE1567.seq Check: 9057 from: 1 to: 5959 pFN21AE1567 5959 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2349 2616 3302 1937 Size: 412 267 686 4594 Fragments arranged by size: 4594 686 412 267 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 3261 4022 5406 5674 1632 Size: 514 1115 761 1384 268 1917 Fragments arranged by size: 1917 1384 1115 761 514 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 2154 4858 5835 1203 Size: 951 2704 977 1327 Fragments arranged by size: 2704 1327 977 951 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2543 1166 Size: 1377 4582 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 2414 1184 Size: 1020 210 4729 Fragments arranged by size: 4729 1020 210 AlwNI CAG_nnn'CTG Cuts at: 3702 5274 3702 Size: 1572 4387 ApaI G_GGCC'C Cuts at: 2746 2746 Size: 5959 ApaLI G'TGCA_C Cuts at: 2389 4016 5172 2389 Size: 1627 1156 3176 Fragments arranged by size: 3176 1627 1156 ApoI r'AATT_y Cuts at: 1493 2091 2507 2765 3268 3494 3646 5755 1493 Size: 598 416 258 503 226 152 2109 1697 Fragments arranged by size: 2109 1697 598 503 416 258 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 2367 3289 3656 2367 Size: 922 367 4670 Fragments arranged by size: 4670 922 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3042 3280 4735 618 Size: 325 217 627 1255 238 1455 1842 Fragments arranged by size: 1842 1455 1255 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 2220 2556 2746 3172 3278 1391 Size: 829 336 190 426 106 4072 Fragments arranged by size: 4072 829 426 336 190 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2326 2360 4150 4184 1903 Size: 34 50 34 305 34 1790 34 3678 Fragments arranged by size: 3678 1790 305 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2280 1548 Size: 732 5227 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2843 4570 136 Size: 107 122 71 631 605 1171 1727 1525 Fragments arranged by size: 1727 1525 1171 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3137 3376 3137 Size: 239 5720 Bme1580I G_kGCm'C Cuts at: 1790 2193 2393 2746 3047 4020 5176 1790 Size: 403 200 353 301 973 1156 2573 Fragments arranged by size: 2573 1156 973 403 353 301 200 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2491 2035 Size: 456 5503 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2234 3212 4619 1239 Size: 954 41 978 1407 2579 Fragments arranged by size: 2579 1407 978 954 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2539 3096 4622 882 Size: 617 1040 557 1526 2219 Fragments arranged by size: 2219 1526 1040 617 557 BsaAI yAC'GTr Cuts at: 493 1204 2029 2188 2673 3750 493 Size: 711 825 159 485 1077 2702 Fragments arranged by size: 2702 1077 825 711 485 159 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3766 4148 4783 275 Size: 53 83 186 936 2233 382 635 1451 Fragments arranged by size: 2233 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2364 4392 4790 5064 5211 5622 2364 Size: 2028 398 274 147 411 2701 Fragments arranged by size: 2701 2028 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5959 BseYI C'CCAG_C Cuts at: 2064 2521 5162 5658 2064 Size: 457 2641 496 2365 Fragments arranged by size: 2641 2365 496 457 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3137 1163 Size: 573 1401 3985 Fragments arranged by size: 3985 1401 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2544 3409 4170 5198 1540 Size: 275 186 543 865 761 1028 2301 Fragments arranged by size: 2301 1028 865 761 543 275 186 BsiHKAI G_wGCw'C Cuts at: 2393 4020 4105 5176 2393 Size: 1627 85 1071 3176 Fragments arranged by size: 3176 1627 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2220 2393 2556 2746 3047 3172 Size: 399 403 27 173 163 190 301 125 Cuts at: 3172 3278 4020 4105 5176 1391 Size: 106 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 301 190 173 163 125 106 85 27 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2470 3846 1394 Size: 429 647 1376 3507 Fragments arranged by size: 3507 1376 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2062 4454 4628 65 Size: 1183 814 2392 174 1396 Fragments arranged by size: 2392 1396 1183 814 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 2958 96 Size: 2862 3097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2852 4019 5031 2852 Size: 1167 1012 3780 Fragments arranged by size: 3780 1167 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2061 794 Size: 1267 4692 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 2426 2545 2908 2426 Size: 119 363 5477 Fragments arranged by size: 5477 363 119 BstYI r'GATC_y Cuts at: 1972 2367 2383 3289 3656 4041 4058 5510 5596 Size: 395 16 906 367 385 17 1452 86 Cuts at: 5596 5608 5954 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 906 395 385 367 346 86 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 2538 2544 2865 2929 3084 3147 3225 Size: 552 1473 6 321 64 155 63 78 Cuts at: 3225 513 Size: 3247 Fragments arranged by size: 3247 1473 552 321 155 78 64 63 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2983 3139 487 Size: 683 56 1757 156 3307 Fragments arranged by size: 3307 1757 683 156 56 BtsI GCAGTG_nn' Cuts at: 2205 3475 4269 4289 2205 Size: 1270 794 20 3875 Fragments arranged by size: 3875 1270 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2541 2811 Size: 54 1425 50 275 18 471 240 270 Cuts at: 2811 2994 3108 3150 3406 4295 5924 8 Size: 183 114 42 256 889 1629 43 Fragments arranged by size: 1629 1425 889 471 275 270 256 240 183 114 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 2541 3406 1537 Size: 275 729 865 4090 Fragments arranged by size: 4090 865 729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 3201 3889 1353 Size: 914 934 688 3423 Fragments arranged by size: 3423 934 914 688 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2396 4546 4920 5066 387 Size: 1064 138 807 2150 374 146 1280 Fragments arranged by size: 2150 1280 1064 807 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2193 2234 3212 3261 4022 4619 Size: 393 514 47 41 978 49 761 597 Cuts at: 4619 5406 5674 1239 Size: 787 268 1524 Fragments arranged by size: 1524 978 787 761 597 514 393 268 49 47 41 EcoNI CCTnn'n_nnAGG Cuts at: 3110 3110 Size: 5959 EcoO109I rG'GnC_Cy Cuts at: 2742 2743 2742 Size: 1 5958 EcoRI G'AATT_C Cuts at: 2091 2765 3268 3646 2091 Size: 674 503 378 4404 Fragments arranged by size: 4404 674 503 378 FspI TGC'GCA Cuts at: 2240 4465 2240 Size: 2225 3734 FspAI rTGC'GCAy Cuts at: 2240 2240 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 5106 1678 Size: 3428 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2846 2878 4600 Size: 32 135 32 462 32 780 32 1722 Cuts at: 4600 4632 4674 4706 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1722 780 462 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 2617 2989 3303 3549 669 Size: 988 281 412 267 372 314 246 3079 Fragments arranged by size: 3079 988 412 372 314 281 267 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2813 3110 10 Size: 54 1425 343 981 297 2859 Fragments arranged by size: 2859 1425 981 343 297 54 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3917 4276 4435 518 Size: 648 1377 1374 359 159 2042 Fragments arranged by size: 2042 1377 1374 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2278 2860 3121 3375 4052 5200 5445 1606 Size: 672 582 261 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 582 261 254 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 2639 387 Size: 2252 3707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 2956 3136 3317 4862 1132 Size: 97 1727 180 181 1545 2229 Fragments arranged by size: 2229 1727 1545 181 180 97 PasI CC'CwG_GG Cuts at: 1348 3095 1348 Size: 1747 4212 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PfoI T'CCnGG_A Cuts at: 2358 2358 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2029 2188 2029 Size: 159 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2640 1613 Size: 1027 4932 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2742 2742 Size: 5959 PstI C_TGCA'G Cuts at: 830 2403 3311 830 Size: 1573 908 3478 Fragments arranged by size: 3478 1573 908 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2278 2860 2278 Size: 582 5377 SalI G'TCGA_C Cuts at: 1936 2348 2615 3301 1936 Size: 412 267 686 4594 Fragments arranged by size: 4594 686 412 267 SapI GCTCTTCn'nnn_ Cuts at: 2267 2267 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SexAI A'CCwGG_T Cuts at: 2352 2352 Size: 5959 SfcI C'TryA_G Cuts at: 826 1045 2251 2399 3307 4442 5123 5314 826 Size: 219 1206 148 908 1135 681 191 1471 Fragments arranged by size: 1471 1206 1135 908 681 219 191 148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4063 4964 5226 820 Size: 197 926 2120 901 262 1553 Fragments arranged by size: 2120 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3136 3317 1132 Size: 2004 181 3774 Fragments arranged by size: 3774 2004 181 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 2846 3164 1517 Size: 1329 318 4312 Fragments arranged by size: 4312 1329 318 StyI C'CwwG_G Cuts at: 513 1065 3111 3398 513 Size: 552 2046 287 3074 Fragments arranged by size: 3074 2046 552 287 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 2591 3986 4003 4156 4341 4786 Size: 1255 703 308 1395 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1395 1255 703 445 308 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 2937 2958 Size: 274 80 33 51 1443 96 864 21 Cuts at: 2958 4205 5917 96 Size: 1247 1712 138 Fragments arranged by size: 1712 1443 1247 864 274 138 96 80 51 33 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3045 3077 5919 5951 3045 Size: 32 2842 32 3053 Fragments arranged by size: 3053 2842 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2354 1316 Size: 1038 4921 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2545 2545 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI