(Linear) (Six-base) MAPSORT of: KIEE1567.seq Check: 7326 from: 1 to: 1254 KIEE1567.orf With 182 enzymes: SgfI * July 15, 2010 18:42 .. AccI GT'mk_AC Cuts at: 0 344 611 1254 Size: 344 267 643 Fragments arranged by size: 643 344 267 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 1254 Size: 141 1113 AflIII A'CryG_T Cuts at: 0 149 1254 Size: 149 1105 AleI CACnn'nnGTG Cuts at: 0 538 1254 Size: 538 716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 409 1254 Size: 199 210 845 Fragments arranged by size: 845 210 199 ApaI G_GGCC'C Cuts at: 0 741 1254 Size: 741 513 ApaLI G'TGCA_C Cuts at: 0 384 1254 Size: 384 870 ApoI r'AATT_y Cuts at: 0 86 502 760 1254 Size: 86 416 258 494 Fragments arranged by size: 494 416 258 86 BamHI G'GATC_C Cuts at: 0 362 1254 Size: 362 892 BanI G'GyrC_C Cuts at: 0 1037 1254 Size: 1037 217 BanII G_rGCy'C Cuts at: 0 215 551 741 1167 1254 Size: 215 336 190 426 87 Fragments arranged by size: 426 336 215 190 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 321 355 1254 Size: 321 34 899 Fragments arranged by size: 899 321 34 BclI T'GATC_A Cuts at: 0 275 1254 Size: 275 979 BglI GCCn_nnn'nGGC Cuts at: 0 838 1254 Size: 838 416 BlpI GC'TnA_GC Cuts at: 0 1132 1254 Size: 1132 122 Bme1580I G_kGCm'C Cuts at: 0 188 388 741 1042 1254 Size: 188 200 353 301 212 Fragments arranged by size: 353 301 212 200 188 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 486 1254 Size: 30 456 768 Fragments arranged by size: 768 456 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 229 1207 1254 Size: 188 41 978 47 Fragments arranged by size: 978 188 47 41 BsaI GGTCTCn'nnnn_ Cuts at: 0 534 1091 1254 Size: 534 557 163 Fragments arranged by size: 557 534 163 BsaAI yAC'GTr Cuts at: 0 24 183 668 1254 Size: 24 159 485 586 Fragments arranged by size: 586 485 159 24 BsaWI w'CCGG_w Cuts at: 0 359 1254 Size: 359 895 BseYI C'CCAG_C Cuts at: 0 59 516 1254 Size: 59 457 738 Fragments arranged by size: 738 457 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 1254 Size: 1132 122 BsiEI CG_ry'CG Cuts at: 0 539 1254 Size: 539 715 BsiHKAI G_wGCw'C Cuts at: 0 388 1254 Size: 388 866 Bsp1286I G_dGCh'C Cuts at: 0 188 215 388 551 741 1042 1167 1254 Size: 188 27 173 163 190 301 125 87 Fragments arranged by size: 301 190 188 173 163 125 87 27 BsrBI CCG'CTC Cuts at: 0 465 1254 Size: 465 789 BsrDI GCAATG_nn' Cuts at: 0 57 1254 Size: 57 1197 BsrGI T'GTAC_A Cuts at: 0 953 1254 Size: 953 301 BssSI C'ACGA_G Cuts at: 0 847 1254 Size: 847 407 BstAPI GCAn_nnn'nTGC Cuts at: 0 56 1254 Size: 56 1198 BstXI CCAn_nnnn'nTGG Cuts at: 0 421 540 903 1254 Size: 421 119 363 351 Fragments arranged by size: 421 363 351 119 BstYI r'GATC_y Cuts at: 0 362 378 1254 Size: 362 16 876 Fragments arranged by size: 876 362 16 BtgI C'CryG_G Cuts at: 0 533 539 860 924 1079 1142 1220 1254 Size: 533 6 321 64 155 63 78 34 Fragments arranged by size: 533 321 155 78 64 63 34 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 978 1134 1254 Size: 978 156 120 Fragments arranged by size: 978 156 120 BtsI GCAGTG_nn' Cuts at: 0 200 1254 Size: 200 1054 EaeI y'GGCC_r Cuts at: 0 296 536 806 989 1103 1145 1254 Size: 296 240 270 183 114 42 109 Fragments arranged by size: 296 270 240 183 114 109 42 EagI C'GGCC_G Cuts at: 0 536 1254 Size: 536 718 EarI CTCTTCn'nnn_ Cuts at: 0 262 1196 1254 Size: 262 934 58 Fragments arranged by size: 934 262 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 391 1254 Size: 391 863 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 188 229 1207 1254 Size: 141 47 41 978 47 Fragments arranged by size: 978 141 47 47 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 1105 1254 Size: 1105 149 EcoO109I rG'GnC_Cy Cuts at: 0 737 738 1254 Size: 737 1 516 Fragments arranged by size: 737 516 1 EcoRI G'AATT_C Cuts at: 0 86 760 1254 Size: 86 674 494 Fragments arranged by size: 674 494 86 FspI TGC'GCA Cuts at: 0 235 1254 Size: 235 1019 FspAI rTGC'GCAy Cuts at: 0 235 1254 Size: 235 1019 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 61 841 873 1254 Size: 29 32 780 32 381 Fragments arranged by size: 780 381 32 32 29 HincII GTy'rAC Cuts at: 0 345 612 984 1254 Size: 345 267 372 270 Fragments arranged by size: 372 345 270 267 MscI TGG'CCA Cuts at: 0 808 1105 1254 Size: 808 297 149 Fragments arranged by size: 808 297 149 MslI CAynn'nnrTG Cuts at: 0 538 1254 Size: 538 716 MspA1I CmG'CkG Cuts at: 0 273 855 1116 1254 Size: 273 582 261 138 Fragments arranged by size: 582 273 261 138 NdeI CA'TA_TG Cuts at: 0 634 1254 Size: 634 620 NspI r_CATG'y Cuts at: 0 951 1131 1254 Size: 951 180 123 Fragments arranged by size: 951 180 123 PasI CC'CwG_GG Cuts at: 0 1090 1254 Size: 1090 164 PfoI T'CCnGG_A Cuts at: 0 353 1254 Size: 353 901 PmlI CAC'GTG Cuts at: 0 24 183 1254 Size: 24 159 1071 Fragments arranged by size: 1071 159 24 PshAI GACnn'nnGTC Cuts at: 0 635 1254 Size: 635 619 PspOMI G'GGCC_C Cuts at: 0 737 1254 Size: 737 517 PstI C_TGCA'G Cuts at: 0 398 1254 Size: 398 856 PvuII CAG'CTG Cuts at: 0 273 855 1254 Size: 273 582 399 Fragments arranged by size: 582 399 273 SalI G'TCGA_C Cuts at: 0 343 610 1254 Size: 343 267 644 Fragments arranged by size: 644 343 267 SapI GCTCTTCn'nnn_ Cuts at: 0 262 1254 Size: 262 992 SexAI A'CCwGG_T Cuts at: 0 347 1254 Size: 347 907 SfcI C'TryA_G Cuts at: 0 246 394 1254 Size: 246 148 860 Fragments arranged by size: 860 246 148 SphI G_CATG'C Cuts at: 0 1131 1254 Size: 1131 123 StuI AGG'CCT Cuts at: 0 841 1159 1254 Size: 841 318 95 Fragments arranged by size: 841 318 95 StyI C'CwwG_G Cuts at: 0 1106 1254 Size: 1106 148 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 278 586 1254 Size: 278 308 668 Fragments arranged by size: 668 308 278 TatI w'GTAC_w Cuts at: 0 68 932 953 1254 Size: 68 864 21 301 Fragments arranged by size: 864 301 68 21 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1040 1072 1254 Size: 1040 32 182 Fragments arranged by size: 1040 182 32 Tth111I GACn'n_nGTC Cuts at: 0 349 1254 Size: 349 905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 540 1254 Size: 540 714 Enzymes that do cut: AccI AcuI AflIII AleI AlfI ApaI ApaLI ApoI BamHI BanI BanII BcgI BclI BglI BlpI Bme1580I BplI BpmI BsaI BsaAI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI Hin4I HincII MscI MslI MspA1I NdeI NspI PasI PfoI PmlI PshAI PspOMI PstI PvuII SalI SapI SexAI SfcI SphI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BciVI BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FalI FseI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI