################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BbsI BbvCI BcgI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI KpnI MamI MfeI MluI MroI MslI MunI NaeI NdeI NgoMIV NruI NsiI NspI NspV PacI PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 523 GTAGAC GT^[AC][GT]AC AcsI 391 GAATTC [GA]^AATT[CT] AcyI 242 GGCGCC G[GA]^CG[CT]C AlwNI 548 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 167 GGGCCC GGGCC^C ApoI 391 GAATTC [GA]^AATT[CT] AscI 159 GGCGCGCC GG^CGCGCC AspHI 1200 GAGCAC G[AT]GC[AT]^C AvaI 1220 CTCGAG C^[CT]CG[GA]G AvaII 6 GGTCC G^G[AT]CC BalI 1191 TGGCCA TGG^CCA BamHI 403 GGATCC G^GATCC BanI 242 GGCGCC G^G[CT][GA]CC BbeI 242 GGCGCC GGCGC^C BglI 99 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 486 AGATCT A^GATCT BsaHI 242 GGCGCC G[GA]^CG[CT]C BseRI 121 CTCCTC GAGGAG(10/8) BsgI 528 CTGCAC GTGCAG(16/14) BsiHKAI 1200 GAGCAC G[AT]GC[AT]^C BsmAI 504 GTCTC GTCTC(1/5) BsmFI 7 GTCCC GGGAC(10/14) BsoBI 1220 CTCGAG C^[CT]CG[GA]G BstNI 14 CCAGG CC^[AT]GG BstXI 885 CCATCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 5 AGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 343 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 5 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 391 GAATTC G^AATTC EcoRII 14 CCAGG ^CC[AT]GG Hin1I 242 GGCGCC G[GA]^CG[CT]C HindIII 671 AAGCTT A^AGCTT HinfI 426 GAATC G^A[ACGT]TC KasI 242 GGCGCC G^GCGCC MaeII 179 ACGT A^CGT MluNI 1191 TGGCCA TGG^CCA MscI 1191 TGGCCA TGG^CCA MvaI 14 CCAGG CC^[AT]GG NarI 242 GGCGCC GG^CGCC NcoI 794 CCATGG C^CATGG NheI 111 GCTAGC G^CTAGC PaeR7I 1220 CTCGAG C^TCGAG PflMI 547 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 5 AGGTCCC [GA]G^G[AT]CC[CT] PstI 980 CTGCAG CTGCA^G PvuII 983 CAGCTG CAG^CTG SapI 850 GAAGAGC GCTCTTC(1/4) ScaI 497 AGTACT AGT^ACT SfoI 242 GGCGCC GGC^GCC TfiI 426 GAATC G^A[AT]TC Van91I 547 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1220 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### BanII 167 GGGCCC G[GA]GC[CT]^C 720 GAGCCC BclI 1062 TGATCA T^GATCA 1101 TGATCA BfaI 112 CTAG C^TAG 599 CTAG BsiEI 91 CGGCCG CG[GA][CT]^CG 97 CGGCCG BsrBI 50 CCGCTC CCGCTC(-3/-3) 118 CCGCTC BssHII 160 GCGCGC G^CGCGC 322 GCGCGC BstF5I 435 GGATG GGATG(2/0) 639 GGATG DraI 268 TTTAAA TTT^AAA 675 TTTAAA EagI 91 CGGCCG C^GGCCG 97 CGGCCG EclXI 91 CGGCCG C^GGCCG 97 CGGCCG Eco52I 91 CGGCCG C^GGCCG 97 CGGCCG EcoT14I 794 CCATGG C^C[AT][AT]GG 1041 CCAAGG FbaI 1062 TGATCA T^GATCA 1101 TGATCA FokI 435 GGATG GGATG(9/13) 639 GGATG HaeII 173 AGCGCC [GA]GCGC^[CT] 242 GGCGCC KspI 94 CCGCGG CCGC^GG 103 CCGCGG MaeI 112 CTAG C^TAG 599 CTAG NlaIII 25 CATG CATG^ 795 CATG NotI 90 GCGGCCGC GC^GGCCGC 96 GCGGCCGC SacII 94 CCGCGG CCGC^GG 103 CCGCGG SfaNI 592 GATGC GCATC(5/9) 1216 GCATC SmlI 462 CTTGAG C^T[CT][GA]AG 1220 CTCGAG SspI 752 AATATT AAT^ATT 781 AATATT StyI 794 CCATGG C^C[AT][AT]GG 1041 CCAAGG TaqI 507 TCGA T^CGA 1221 TCGA TspRI 552 CACTG CA[GC]TG[ACGT][ACGT]^ 1188 CAGTG TthHB8I 507 TCGA T^CGA 1221 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbvI BciVI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstUI BstYI Cac8I CfoI Cfr13I DpnII DsaI EaeI EarI FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvnI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I XhoII