(Circular) (Six-base) MAPSORT of: pFN21AE0732.seq Check: 870 from: 1 to: 5965 pFN21AE0732 5965 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2529 3308 1937 Size: 592 779 4594 Fragments arranged by size: 4594 779 592 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 4028 5412 5680 1632 Size: 1128 1268 1384 268 1917 Fragments arranged by size: 1917 1384 1268 1128 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 4864 5841 1203 Size: 3661 977 1327 Fragments arranged by size: 3661 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2358 1184 Size: 1174 4791 AlwNI CAG_nnn'CTG Cuts at: 2558 3708 5280 2558 Size: 1150 1572 3243 Fragments arranged by size: 3243 1572 1150 ApaI G_GGCC'C Cuts at: 2176 2176 Size: 5965 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 2396 3274 3500 3652 5761 1493 Size: 903 878 226 152 2109 1697 Fragments arranged by size: 2109 1697 903 878 226 152 AscI GG'CGCG_CC Cuts at: 2165 2165 Size: 5965 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 3225 3290 1870 Size: 73 1282 65 4545 Fragments arranged by size: 4545 1282 73 65 BamHI G'GATC_C Cuts at: 2408 3295 3662 2408 Size: 887 367 4711 Fragments arranged by size: 4711 887 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2247 3286 4741 618 Size: 325 217 627 460 1039 1455 1842 Fragments arranged by size: 1842 1455 1039 627 460 325 217 BanII G_rGCy'C Cuts at: 1391 2176 2729 3284 1391 Size: 785 553 555 4072 Fragments arranged by size: 4072 785 555 553 BbeI G_GCGC'C Cuts at: 2251 2251 Size: 5965 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4994 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2470 2725 2797 3857 5067 2470 Size: 255 72 1060 1210 3368 Fragments arranged by size: 3368 1210 1060 255 72 BclI T'GATC_A Cuts at: 1548 3067 3106 1548 Size: 1519 39 4407 Fragments arranged by size: 4407 1519 39 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2110 4576 136 Size: 107 122 71 631 605 438 2466 1525 Fragments arranged by size: 2466 1525 631 605 438 122 107 71 BglII A'GATC_T Cuts at: 2491 5960 2491 Size: 3469 2496 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 2176 4026 5182 1790 Size: 386 1850 1156 2573 Fragments arranged by size: 2573 1850 1156 386 BmgBI CAC'GTC Cuts at: 1318 2185 1318 Size: 867 5098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2568 3153 3696 Size: 416 53 83 399 24 1151 585 543 Cuts at: 3696 4654 442 Size: 958 1753 Fragments arranged by size: 1753 1151 958 585 543 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2120 1056 Size: 1064 4901 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4625 1239 Size: 3386 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2488 4090 4955 5253 2488 Size: 1602 865 298 3200 Fragments arranged by size: 3200 1602 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2248 3772 4154 4789 Size: 53 83 186 936 715 1524 382 635 Cuts at: 4789 275 Size: 1451 Fragments arranged by size: 1524 1451 936 715 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4398 4796 5070 5217 5628 4398 Size: 398 274 147 411 4735 Fragments arranged by size: 4735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5740 Fragments arranged by size: 5740 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 1186 Size: 931 5034 BseYI C'CCAG_C Cuts at: 2144 2175 2193 2472 5168 5664 2144 Size: 31 18 279 2696 496 2445 Fragments arranged by size: 2696 2445 496 279 31 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2518 1163 Size: 573 782 4610 Fragments arranged by size: 4610 782 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2099 2105 3415 4176 5204 1540 Size: 275 186 98 6 1310 761 1028 2301 Fragments arranged by size: 2301 1310 1028 761 275 186 98 6 BsiHKAI G_wGCw'C Cuts at: 3209 4026 4111 5182 3209 Size: 817 85 1071 3992 Fragments arranged by size: 3992 1071 817 85 BsmI GAATG_Cn' Cuts at: 3476 3569 3476 Size: 93 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2176 2729 3209 3284 4026 4111 5182 Size: 399 386 553 480 75 742 85 1071 Cuts at: 5182 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 553 480 399 386 85 75 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 844 Size: 2476 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2057 2125 3852 1394 Size: 429 234 68 1727 3507 Fragments arranged by size: 3507 1727 429 234 68 BsrDI GCAATG_nn' Cuts at: 65 1248 4460 4634 65 Size: 1183 3212 174 1396 Fragments arranged by size: 3212 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4609 4796 1700 Size: 110 2799 187 2869 Fragments arranged by size: 2869 2799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 2165 2327 1925 Size: 240 162 5563 Fragments arranged by size: 5563 240 162 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5965 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstXI CCAn_nnnn'nTGG Cuts at: 2897 2897 Size: 5965 BstYI r'GATC_y Cuts at: 1972 2254 2408 2491 2548 2659 3295 3662 4047 Size: 282 154 83 57 111 636 367 385 Cuts at: 4047 4064 5516 5602 5614 5960 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 636 385 367 346 282 154 111 86 83 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2099 2108 2799 513 Size: 552 939 95 9 691 3679 Fragments arranged by size: 3679 939 691 552 95 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 3481 4275 4295 3481 Size: 794 20 5151 Fragments arranged by size: 5151 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 2275 2682 3269 2275 Size: 407 587 4971 Fragments arranged by size: 4971 587 407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2354 4972 809 Size: 507 1038 2618 1802 Fragments arranged by size: 2618 1802 1038 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2102 3019 Size: 54 1425 50 275 18 266 6 917 Cuts at: 3019 3196 3412 4301 5930 8 Size: 177 216 889 1629 43 Fragments arranged by size: 1629 1425 917 889 275 266 216 177 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2096 2102 3412 1537 Size: 275 284 6 1310 4090 Fragments arranged by size: 4090 1310 284 275 6 EarI CTCTTCn'nnn_ Cuts at: 1353 2264 2818 2850 2971 3895 1353 Size: 911 554 32 121 924 3423 Fragments arranged by size: 3423 924 911 554 121 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2140 2252 2287 3144 4552 4926 Size: 1064 138 551 112 35 857 1408 374 Cuts at: 4926 5072 387 Size: 146 1280 Fragments arranged by size: 1408 1280 1064 857 551 374 146 138 112 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2760 4028 4625 5412 5680 1239 Size: 393 1128 1268 597 787 268 1524 Fragments arranged by size: 1524 1268 1128 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2011 2011 Size: 5965 EcoRI G'AATT_C Cuts at: 2396 3274 3652 2396 Size: 878 378 4709 Fragments arranged by size: 4709 878 378 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 2182 2251 5112 1678 Size: 504 69 2861 2531 Fragments arranged by size: 2861 2531 504 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2416 2432 2448 2492 Size: 32 135 32 828 16 16 16 44 Cuts at: 2492 2524 4606 4638 4680 4712 1373 Size: 32 2082 32 42 32 2626 Fragments arranged by size: 2626 2082 828 135 44 42 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3309 3555 669 Size: 988 281 1371 246 3079 Fragments arranged by size: 3079 1371 988 281 246 HindIII A'AGCT_T Cuts at: 748 2676 748 Size: 1928 4037 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KasI G'GCGC_C Cuts at: 2247 2247 Size: 5965 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5079 5263 1305 Size: 3774 184 2007 Fragments arranged by size: 3774 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3198 10 Size: 54 1425 343 1366 2777 Fragments arranged by size: 2777 1425 1366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3923 4282 4441 518 Size: 648 2757 359 159 2042 Fragments arranged by size: 2757 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2110 2306 2990 3381 4058 5206 5451 Size: 495 9 196 684 391 677 1148 245 Cuts at: 5451 1606 Size: 2120 Fragments arranged by size: 2120 1148 684 677 495 391 245 196 9 NarI GG'CG_CC Cuts at: 2248 2248 Size: 5965 NcoI C'CATG_G Cuts at: 513 1065 2004 2799 513 Size: 552 939 795 3679 Fragments arranged by size: 3679 939 795 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NheI G'CTAG_C Cuts at: 1052 2116 1052 Size: 1064 4901 NotI GC'GGCC_GC Cuts at: 2096 2102 3412 2096 Size: 6 1310 4649 Fragments arranged by size: 4649 1310 6 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 2558 1108 Size: 1450 4515 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2011 Size: 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PspOMI G'GGCC_C Cuts at: 2172 2172 Size: 5965 PstI C_TGCA'G Cuts at: 830 2989 3317 830 Size: 2159 328 3478 Fragments arranged by size: 3478 2159 328 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2990 2990 Size: 5965 SacII CC_GC'GG Cuts at: 2102 2111 2102 Size: 9 5956 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SapI GCTCTTCn'nnn_ Cuts at: 2850 2850 Size: 5965 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 2504 4213 5925 2504 Size: 1709 1712 2544 Fragments arranged by size: 2544 1712 1709 SfcI C'TryA_G Cuts at: 826 1045 2985 3115 3190 3313 4448 5129 5320 Size: 219 1940 130 75 123 1135 681 191 Cuts at: 5320 826 Size: 1471 Fragments arranged by size: 1940 1471 1135 681 219 191 130 123 75 SfoI GGC'GCC Cuts at: 2249 2249 Size: 5965 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2467 3225 4069 4970 5232 820 Size: 197 926 524 758 844 901 262 1553 Fragments arranged by size: 1553 926 901 844 758 524 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 2759 2788 3889 5 Size: 47 2707 29 1101 2081 Fragments arranged by size: 2707 2081 1101 47 29 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2004 2799 3046 3404 513 Size: 552 939 795 247 358 3074 Fragments arranged by size: 3074 939 795 552 358 247 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 3992 4009 4162 4347 4792 325 Size: 1255 1125 1287 17 153 185 445 1498 Fragments arranged by size: 1498 1287 1255 1125 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 2502 4211 Size: 274 80 33 51 1443 402 123 1709 Cuts at: 4211 5923 96 Size: 1712 138 Fragments arranged by size: 1712 1709 1443 402 274 138 123 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 3225 1943 Size: 1282 4683 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI