################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbeI BbrPI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SalI SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI TfiI Tru9I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AspI 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 767 GGATCC G^GATCC BbsI 1343 GTCTTC GAAGAC(2/6) BbvCI 1991 CCTCAGC CCTCAGC(-5/-2) BcgI 1602 CGAACAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1741 GGATAC GTATCC(6/5) BclI 1595 TGATCA T^GATCA BlpI 1154 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1154 GCTCAGC GC^T[ACGT]AGC BpuAI 1343 GTCTTC GAAGAC(2/6) BseRI 1802 GAGGAG GAGGAG(10/8) BsmI 560 GAATGC GAATGC(1/-1) BspMI 1330 GCAGGT ACCTGC(4/8) BssHII 12 GCGCGC G^CGCGC BstXI 1373 CCAGCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1154 GCTCAGC GC^T[ACGT]AGC EagI 62 CGGCCG C^GGCCG EclXI 62 CGGCCG C^GGCCG Eco52I 62 CGGCCG C^GGCCG FbaI 1595 TGATCA T^GATCA FseI 1077 GGCCGGCC GGCCGG^CC MaeII 1274 ACGT A^CGT MboII 1344 TCTTC GAAGA(8/7) NotI 61 GCGGCCGC GC^GGCCGC PaeR7I 1727 CTCGAG C^TCGAG PflFI 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 1673 CAGCTG CAG^CTG SbfI 1497 CCTGCAGG CCTGCA^GG SexAI 852 ACCTGGT A^CC[AT]GGT SmlI 1727 CTCGAG C^T[CT][GA]AG Sse8387I 1497 CCTGCAGG CCTGCA^GG StuI 351 AGGCCT AGG^CCT Tsp509I 1608 AATT ^AATT Tth111I 851 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1754 CCACTGGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1727 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 1099 GGCGTC G[GA]^CG[CT]C 1504 GGCGTC AocI 1309 CCTAAGG CC^T[ACGT]AGG 1629 CCTAAGG AspHI 974 GTGCTC G[AT]GC[AT]^C 1907 GTGCTC BalI 945 TGGCCA TGG^CCA 1143 TGGCCA BsaHI 1099 GGCGTC G[GA]^CG[CT]C 1504 GGCGTC BsiEI 62 CGGCCG CG[GA][CT]^CG 1281 CGACCG BsiHKAI 974 GTGCTC G[AT]GC[AT]^C 1907 GTGCTC BsrFI 655 GCCGGC [GA]^CCGG[CT] 1078 GCCGGC BstF5I 252 GGATG GGATG(2/0) 1592 GGATG Bsu36I 1309 CCTAAGG CC^T[ACGT]AGG 1629 CCTAAGG Cfr10I 655 GCCGGC [GA]^CCGG[CT] 1078 GCCGGC Eco81I 1309 CCTAAGG CC^T[ACGT]AGG 1629 CCTAAGG FokI 252 GGATG GGATG(9/13) 1592 GGATG HgaI 1100 GCGTC GACGC(5/10) 1505 GCGTC Hin1I 1099 GGCGTC G[GA]^CG[CT]C 1504 GGCGTC MluNI 945 TGGCCA TGG^CCA 1143 TGGCCA MscI 945 TGGCCA TGG^CCA 1143 TGGCCA NaeI 655 GCCGGC GCC^GGC 1078 GCCGGC NgoMIV 655 GCCGGC G^CCGGC 1078 GCCGGC NspI 902 GCATGC [GA]CATG^[CT] 1118 GCATGC PshAI 733 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1282 GACCGGGGTC PstI 679 CTGCAG CTGCA^G 1498 CTGCAG SfcI 679 CTGCAG C^T[GA][CT]AG 1498 CTGCAG SphI 902 GCATGC GCATG^C 1118 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstEII BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeIII MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI SacII Sau3AI Sau96I ScrFI SfaNI SfiI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI