(Circular) (Six-base) MAPSORT of: pFN21AE4559.seq Check: 1191 from: 1 to: 6757 pFN21AE4559 6757 bp With 182 enzymes: SgfI * March 28, 2012 14:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4567 278 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4100 1937 Size: 2163 4594 Acc65I G'GTAC_C Cuts at: 4078 4078 Size: 6757 AclI AA'CG_TT Cuts at: 1563 4884 5257 1563 Size: 3321 373 3063 Fragments arranged by size: 3321 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 2523 3867 4820 6204 6472 1632 Size: 872 19 1344 953 1384 268 1917 Fragments arranged by size: 1917 1384 1344 953 872 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6560 AflIII A'CryG_T Cuts at: 1203 5656 6633 1203 Size: 4453 977 1327 Fragments arranged by size: 4453 1327 977 AgeI A'CCGG_T Cuts at: 5588 5588 Size: 6757 AhdI GACnn_n'nnGTC Cuts at: 5486 5486 Size: 6757 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6757 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6757 AlwNI CAG_nnn'CTG Cuts at: 4500 6072 4500 Size: 1572 5185 ApaI G_GGCC'C Cuts at: 2184 2203 2559 2658 2693 2730 2819 2999 3172 Size: 19 356 99 35 37 89 180 173 Cuts at: 3172 3431 3486 3696 3786 3864 3970 2184 Size: 259 55 210 90 78 106 4971 Fragments arranged by size: 4971 356 259 210 180 173 106 99 90 89 78 55 37 35 19 ApaLI G'TGCA_C Cuts at: 4814 5970 4814 Size: 1156 5601 ApoI r'AATT_y Cuts at: 1493 4066 4292 4444 6553 1493 Size: 2573 226 152 2109 1697 Fragments arranged by size: 2573 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5311 160 Size: 872 4279 1606 Fragments arranged by size: 4279 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2123 2185 2329 2339 2425 2668 Size: 73 108 72 62 144 10 86 243 Cuts at: 2668 2694 2723 3479 3514 3653 3732 3779 4082 Size: 26 29 756 35 139 79 47 303 Cuts at: 4082 1870 Size: 4545 Fragments arranged by size: 4545 756 303 243 144 139 108 86 79 73 72 62 47 35 29 26 10 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3739 3772 3739 Size: 33 6724 BamHI G'GATC_C Cuts at: 2772 4087 4454 2772 Size: 1315 367 5075 Fragments arranged by size: 5075 1315 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 3302 3437 3455 3649 Size: 325 217 627 510 1005 135 18 194 Cuts at: 3649 3887 3956 4078 5533 618 Size: 238 69 122 1455 1842 Fragments arranged by size: 1842 1455 1005 627 510 325 238 217 194 135 122 69 18 BanII G_rGCy'C Cuts at: 1391 2184 2203 2559 2658 2693 2730 2819 2999 Size: 793 19 356 99 35 37 89 180 Cuts at: 2999 3161 3172 3199 3431 3486 3696 3786 3864 Size: 162 11 27 232 55 210 90 78 Cuts at: 3864 3970 4076 1391 Size: 106 106 4072 Fragments arranged by size: 4072 793 356 232 210 180 162 106 106 99 90 89 78 55 37 35 27 19 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3341 928 Size: 971 1442 4344 Fragments arranged by size: 4344 1442 971 BbvCI CC'TCA_GC Cuts at: 3997 3997 Size: 6757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3596 3630 4948 4982 1903 Size: 34 1659 34 1318 34 3678 Fragments arranged by size: 3678 1659 1318 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4649 5859 3740 Size: 909 1210 4638 Fragments arranged by size: 4638 1210 909 BclI T'GATC_A Cuts at: 1548 3600 1548 Size: 2052 4705 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2075 2106 2142 Size: 107 122 71 631 605 403 31 36 Cuts at: 2142 2341 2418 2670 2725 2931 3156 3183 3228 Size: 199 77 252 55 206 225 27 45 Cuts at: 3228 3255 3264 3435 3507 3965 5368 136 Size: 27 9 171 72 458 1403 1525 Fragments arranged by size: 1525 1403 631 605 458 403 252 225 206 199 171 122 107 77 72 71 55 45 36 31 27 27 9 BglII A'GATC_T Cuts at: 6752 6752 Size: 6757 BlpI GC'TnA_GC Cuts at: 3160 4174 3160 Size: 1014 5743 Bme1580I G_kGCm'C Cuts at: 1790 2184 2203 2302 2528 2559 2658 2693 2730 Size: 394 19 99 226 31 99 35 37 Cuts at: 2730 2819 2999 3172 3431 3486 3696 3786 3864 Size: 89 180 173 259 55 210 90 78 Cuts at: 3864 3885 3970 4818 5974 1790 Size: 21 85 848 1156 2573 Fragments arranged by size: 2573 1156 848 394 259 226 210 180 173 99 99 90 89 85 78 55 37 35 31 21 19 BmgBI CAC'GTC Cuts at: 1318 3280 1318 Size: 1962 4795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3380 3771 4488 Size: 416 53 83 399 24 1963 391 717 Cuts at: 4488 5446 442 Size: 958 1753 Fragments arranged by size: 1963 1753 958 717 416 399 391 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6757 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2108 2108 Size: 6757 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2151 3114 3132 5417 1239 Size: 912 963 18 2285 2579 Fragments arranged by size: 2579 2285 963 912 18 Bpu10I CC'TnA_GC Cuts at: 3997 3997 Size: 6757 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4882 5747 6045 4882 Size: 865 298 5594 Fragments arranged by size: 5594 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3234 3315 3768 3846 5420 882 Size: 617 1735 81 453 78 1574 2219 Fragments arranged by size: 2219 1735 1574 617 453 81 78 BsaAI yAC'GTr Cuts at: 493 1204 4548 493 Size: 711 3344 2702 Fragments arranged by size: 3344 2702 711 BsaBI GATnn'nnATC Cuts at: 6751 6751 Size: 6757 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3105 3510 4564 4946 Size: 53 83 186 936 1572 405 1054 382 Cuts at: 4946 5581 275 Size: 635 1451 Fragments arranged by size: 1572 1451 1054 936 635 405 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5190 5588 5862 6009 6420 5190 Size: 398 274 147 411 5527 Fragments arranged by size: 5527 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6532 Fragments arranged by size: 6532 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3822 1186 Size: 2636 4121 BseYI C'CCAG_C Cuts at: 2231 2344 3065 3073 3377 3424 3769 5960 6456 Size: 113 721 8 304 47 345 2191 496 Cuts at: 6456 2231 Size: 2532 Fragments arranged by size: 2532 2191 721 496 345 304 113 47 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 3289 4207 4968 5996 1540 Size: 275 186 69 1219 918 761 1028 2301 Fragments arranged by size: 2301 1219 1028 918 761 275 186 69 BsiHKAI G_wGCw'C Cuts at: 2983 3916 4818 4903 5974 2983 Size: 933 902 85 1071 3766 Fragments arranged by size: 3766 1071 933 902 85 BsmI GAATG_Cn' Cuts at: 2571 4268 4361 2571 Size: 1697 93 4967 Fragments arranged by size: 4967 1697 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2203 2302 2528 2559 2658 2693 Size: 399 394 19 99 226 31 99 35 Cuts at: 2693 2730 2819 2983 2999 3161 3172 3199 3431 Size: 37 89 164 16 162 11 27 232 Cuts at: 3431 3486 3696 3786 3864 3885 3916 3970 4076 Size: 55 210 90 78 21 31 54 106 Cuts at: 4076 4818 4903 5974 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 232 226 210 164 162 106 99 99 90 89 85 78 55 54 37 35 31 31 27 21 19 16 11 BspEI T'CCGG_A Cuts at: 6420 6420 Size: 6757 BspHI T'CATG_A Cuts at: 4646 6376 4646 Size: 1730 5027 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 4112 844 Size: 2482 786 3489 Fragments arranged by size: 3489 2482 786 BsrBI CCG'CTC Cuts at: 1394 1823 2032 2391 3029 4644 1394 Size: 429 209 359 638 1615 3507 Fragments arranged by size: 3507 1615 638 429 359 209 BsrDI GCAATG_nn' Cuts at: 65 1248 5252 5426 65 Size: 1183 4004 174 1396 Fragments arranged by size: 4004 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2660 3083 5401 5588 1700 Size: 110 850 423 2318 187 2869 Fragments arranged by size: 2869 2318 850 423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6757 BssHII G'CGCG_C Cuts at: 1925 2017 1925 Size: 92 6665 BssSI C'ACGA_G Cuts at: 4817 5829 4817 Size: 1012 5745 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6757 BstBI TT'CG_AA Cuts at: 5561 5561 Size: 6757 BstEII G'GTnAC_C Cuts at: 1184 2636 2798 2843 3719 1184 Size: 1452 162 45 876 4222 Fragments arranged by size: 4222 1452 876 162 45 BstXI CCAn_nnnn'nTGG Cuts at: 3385 3385 Size: 6757 BstYI r'GATC_y Cuts at: 1972 2772 3940 3984 4087 4454 4839 4856 6308 Size: 800 1168 44 103 367 385 17 1452 Cuts at: 6308 6394 6406 6752 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1168 800 385 367 346 103 86 44 17 12 Bsu36I CC'TnA_GG Cuts at: 3315 3635 3315 Size: 320 6437 BtgI C'CryG_G Cuts at: 513 1065 2004 2416 2830 3751 513 Size: 552 939 412 414 921 3519 Fragments arranged by size: 3519 939 921 552 414 412 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6018 Fragments arranged by size: 6018 683 56 BtsI GCAGTG_nn' Cuts at: 4273 5067 5087 4273 Size: 794 20 5943 Fragments arranged by size: 5943 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6757 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6722 DraI TTT'AAA Cuts at: 4061 4061 Size: 6757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5764 809 Size: 507 4448 1802 Fragments arranged by size: 4448 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2067 2134 Size: 54 1425 50 275 18 177 60 67 Cuts at: 2134 2883 2923 2950 3148 3901 4204 5093 6722 Size: 749 40 27 198 753 303 889 1629 Cuts at: 6722 8 Size: 43 Fragments arranged by size: 1629 1425 889 753 749 303 275 198 177 67 60 54 50 43 40 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 4204 1537 Size: 275 255 2137 4090 Fragments arranged by size: 4090 2137 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 4687 1353 Size: 3334 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5344 5718 5864 387 Size: 1064 138 3755 374 146 1280 Fragments arranged by size: 3755 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2504 2523 3114 3132 3867 4820 Size: 393 519 353 19 591 18 735 953 Cuts at: 4820 5417 6204 6472 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 953 787 735 597 591 519 393 353 268 19 18 EcoO109I rG'GnC_Cy Cuts at: 2181 2199 2200 2324 2429 2465 2546 2555 2556 Size: 18 1 124 105 36 81 9 1 Cuts at: 2556 2582 2689 2690 2727 2744 2815 2995 2996 Size: 26 107 1 37 17 71 180 1 Cuts at: 2996 3168 3169 3185 3257 3265 3311 3374 3428 Size: 172 1 16 72 8 46 63 54 Cuts at: 3428 3446 3483 3693 3701 3737 3860 3861 3869 Size: 18 37 210 8 36 123 1 8 Cuts at: 3869 3966 2181 Size: 97 4972 Fragments arranged by size: 4972 210 180 172 124 123 107 105 97 81 72 71 63 54 46 37 37 36 36 26 18 18 17 16 9 8 8 8 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 4066 4444 4066 Size: 378 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3660 3660 Size: 6757 FseI GG_CCGG'CC Cuts at: 3087 3087 Size: 6757 FspI TGC'GCA Cuts at: 5263 5263 Size: 6757 HaeII r_GCGC'y Cuts at: 1678 2511 2786 3732 5904 1678 Size: 833 275 946 2172 2531 Fragments arranged by size: 2531 2172 946 833 275 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5398 5430 5472 5504 1373 Size: 32 135 32 3826 32 42 32 2626 Fragments arranged by size: 3826 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4101 4347 669 Size: 988 281 2163 246 3079 Fragments arranged by size: 3079 2163 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6757 HpaI GTT'AAC Cuts at: 4347 4347 Size: 6757 KpnI G_GTAC'C Cuts at: 4082 4082 Size: 6757 MfeI C'AATT_G Cuts at: 4356 4356 Size: 6757 MluI A'CGCG_T Cuts at: 6633 6633 Size: 6757 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3366 3370 5871 6055 1305 Size: 2061 4 2501 184 2007 Fragments arranged by size: 2501 2061 2007 184 4 MscI TGG'CCA Cuts at: 10 64 1489 1832 2952 3150 10 Size: 54 1425 343 1120 198 3617 Fragments arranged by size: 3617 1425 1120 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 4715 5074 5233 518 Size: 648 3549 359 159 2042 Fragments arranged by size: 3549 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2418 2832 3164 3381 3462 3680 3753 4173 Size: 812 414 332 217 81 218 73 420 Cuts at: 4173 4850 5998 6243 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 812 677 420 414 332 245 218 217 81 73 NaeI GCC'GGC Cuts at: 2662 3085 2662 Size: 423 6334 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5266 Fragments arranged by size: 5266 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6757 NgoMIV G'CCGG_C Cuts at: 2660 3083 2660 Size: 423 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6757 NotI GC'GGCC_GC Cuts at: 2067 4204 2067 Size: 2137 4620 NspI r_CATG'y Cuts at: 1132 1229 2911 3127 4115 5660 1132 Size: 97 1682 216 988 1545 2229 Fragments arranged by size: 2229 1682 1545 988 216 97 PasI CC'CwG_GG Cuts at: 1348 2114 2303 2552 2651 2732 3262 3442 3689 Size: 766 189 249 99 81 530 180 247 Cuts at: 3689 3970 1348 Size: 281 4135 Fragments arranged by size: 4135 766 530 281 249 247 189 180 99 81 PciI A'CATG_T Cuts at: 5656 5656 Size: 6757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6757 PfoI T'CCnGG_A Cuts at: 2470 2983 3190 3295 3934 2470 Size: 513 207 105 639 5293 Fragments arranged by size: 5293 639 513 207 105 PmeI GTTT'AAAC Cuts at: 4061 4061 Size: 6757 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4826 4858 6339 6371 4826 Size: 32 1481 32 5212 Fragments arranged by size: 5212 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2324 2429 2465 2546 2582 2744 3374 3446 3701 Size: 105 36 81 36 162 630 72 255 Cuts at: 3701 3737 3869 2324 Size: 36 132 5212 Fragments arranged by size: 5212 630 255 162 132 105 81 72 36 36 36 PshAI GACnn'nnGTC Cuts at: 1613 2742 3291 1613 Size: 1129 549 5079 Fragments arranged by size: 5079 1129 549 PsiI TTA'TAA Cuts at: 4327 4327 Size: 6757 PspOMI G'GGCC_C Cuts at: 2180 2199 2555 2654 2689 2726 2815 2995 3168 Size: 19 356 99 35 37 89 180 173 Cuts at: 3168 3427 3482 3692 3782 3860 3966 2180 Size: 259 55 210 90 78 106 4971 Fragments arranged by size: 4971 356 259 210 180 173 106 99 90 89 78 55 37 35 19 PspXI vC'TCGA_Gb Cuts at: 3732 3732 Size: 6757 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3322 3354 3322 Size: 32 6725 PstI C_TGCA'G Cuts at: 830 2688 3507 4109 830 Size: 1858 819 602 3478 Fragments arranged by size: 3478 1858 819 602 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6757 PvuII CAG'CTG Cuts at: 3680 3680 Size: 6757 SacII CC_GC'GG Cuts at: 2419 2833 3754 2419 Size: 414 921 5422 Fragments arranged by size: 5422 921 414 SalI G'TCGA_C Cuts at: 1936 4099 1936 Size: 2163 4594 SanDI GG'GwC_CC Cuts at: 2324 2582 2744 3374 3446 2324 Size: 258 162 630 72 5635 Fragments arranged by size: 5635 630 258 162 72 SbfI CC_TGCA'GG Cuts at: 3507 4109 3507 Size: 602 6155 ScaI AGT'ACT Cuts at: 5005 6717 5005 Size: 1712 5045 SexAI A'CCwGG_T Cuts at: 2857 2857 Size: 6757 SfcI C'TryA_G Cuts at: 826 1045 2684 3503 4105 5240 5921 6112 826 Size: 219 1639 819 602 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 819 681 602 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2106 2418 2725 3183 3255 3264 2106 Size: 312 307 458 72 9 5599 Fragments arranged by size: 5599 458 312 307 72 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6757 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6757 SmaI CCC'GGG Cuts at: 1872 2125 2187 2331 2341 2427 2670 2696 3481 Size: 253 62 144 10 86 243 26 785 Cuts at: 3481 3516 3655 3781 4084 1872 Size: 35 139 126 303 4545 Fragments arranged by size: 4545 785 303 253 243 144 139 126 86 62 35 26 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 3732 4861 5762 6024 820 Size: 197 926 1789 1129 901 262 1553 Fragments arranged by size: 1789 1553 1129 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4548 493 Size: 4055 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6757 SphI G_CATG'C Cuts at: 1132 2911 3127 4115 1132 Size: 1779 216 988 3774 Fragments arranged by size: 3774 1779 988 216 SrfI GCCC'GGGC Cuts at: 2341 2341 Size: 6757 SspI AAT'ATT Cuts at: 5 52 4681 5 Size: 47 4629 2081 Fragments arranged by size: 4629 2081 47 StuI AGG'CCT Cuts at: 1517 2358 1517 Size: 841 5916 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 2838 2865 3865 3874 4006 Size: 552 939 288 546 27 1000 9 132 Cuts at: 4006 4196 513 Size: 190 3074 Fragments arranged by size: 3074 1000 939 552 546 288 190 132 27 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2502 2817 3065 3455 4784 4801 4954 Size: 1255 922 315 248 390 1329 17 153 Cuts at: 4954 5139 5584 325 Size: 185 445 1498 Fragments arranged by size: 1498 1329 1255 922 445 390 315 248 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5003 6715 96 Size: 274 80 33 51 1443 3026 1712 138 Fragments arranged by size: 3026 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2782 2814 3430 3462 6717 6749 2782 Size: 32 616 32 3255 32 2790 Fragments arranged by size: 3255 2790 616 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2859 1316 Size: 1543 5214 XbaI T'CTAG_A Cuts at: 4093 4093 Size: 6757 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3766 3766 Size: 6757 XhoI C'TCGA_G Cuts at: 1943 3732 1943 Size: 1789 4968 XmaI C'CCGG_G Cuts at: 1870 2123 2185 2329 2339 2425 2668 2694 3479 Size: 253 62 144 10 86 243 26 785 Cuts at: 3479 3514 3653 3779 4082 1870 Size: 35 139 126 303 4545 Fragments arranged by size: 4545 785 303 253 243 144 139 126 86 62 35 26 10 XmnI GAAnn'nnTTC Cuts at: 4886 4886 Size: 6757 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4565 276 Size: 53 83 186 936 3031 2468 Fragments arranged by size: 3031 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PmlI RsrII SacI SapI SfoI SwaI