(Linear) (Six-base) MAPSORT of: KIEE4559.seq Check: 8266 from: 1 to: 2052 KIEE4559.orf With 182 enzymes: SgfI * March 22, 2012 18:22 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 499 518 1862 2052 Size: 499 19 1344 190 Fragments arranged by size: 1344 499 190 19 ApaI G_GGCC'C Cuts at: 0 179 198 554 653 688 725 814 994 Size: 179 19 356 99 35 37 89 180 Cuts at: 994 1167 1426 1481 1691 1781 1859 1965 2052 Size: 173 259 55 210 90 78 106 87 Fragments arranged by size: 356 259 210 180 179 173 106 99 90 89 87 78 55 37 35 19 AvaI C'yCGr_G Cuts at: 0 46 118 180 324 334 420 663 689 Size: 46 72 62 144 10 86 243 26 Cuts at: 689 718 1474 1509 1648 1727 1774 2052 Size: 29 756 35 139 79 47 278 Fragments arranged by size: 756 278 243 144 139 86 79 72 62 47 46 35 29 26 10 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1734 1767 2052 Size: 1734 33 285 Fragments arranged by size: 1734 285 33 BamHI G'GATC_C Cuts at: 0 767 2052 Size: 767 1285 BanI G'GyrC_C Cuts at: 0 292 1297 1432 1450 1644 1882 1951 2052 Size: 292 1005 135 18 194 238 69 101 Fragments arranged by size: 1005 292 238 194 135 101 69 18 BanII G_rGCy'C Cuts at: 0 179 198 554 653 688 725 814 994 Size: 179 19 356 99 35 37 89 180 Cuts at: 994 1156 1167 1194 1426 1481 1691 1781 1859 Size: 162 11 27 232 55 210 90 78 Cuts at: 1859 1965 2052 Size: 106 87 Fragments arranged by size: 356 232 210 180 179 162 106 99 90 89 87 78 55 37 35 27 19 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 1336 2052 Size: 1336 716 BbvCI CC'TCA_GC Cuts at: 0 1992 2052 Size: 1992 60 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1591 1625 2052 Size: 1591 34 427 Fragments arranged by size: 1591 427 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1735 2052 Size: 1735 317 BclI T'GATC_A Cuts at: 0 1595 2052 Size: 1595 457 BglI GCCn_nnn'nGGC Cuts at: 0 70 101 137 336 413 665 720 926 Size: 70 31 36 199 77 252 55 206 Cuts at: 926 1151 1178 1223 1250 1259 1430 1502 1960 Size: 225 27 45 27 9 171 72 458 Cuts at: 1960 2052 Size: 92 Fragments arranged by size: 458 252 225 206 199 171 92 77 72 70 55 45 36 31 27 27 9 BlpI GC'TnA_GC Cuts at: 0 1155 2052 Size: 1155 897 Bme1580I G_kGCm'C Cuts at: 0 179 198 297 523 554 653 688 725 Size: 179 19 99 226 31 99 35 37 Cuts at: 725 814 994 1167 1426 1481 1691 1781 1859 Size: 89 180 173 259 55 210 90 78 Cuts at: 1859 1880 1965 2052 Size: 21 85 87 Fragments arranged by size: 259 226 210 180 179 173 99 99 90 89 87 85 78 55 37 35 31 21 19 BmgBI CAC'GTC Cuts at: 0 1275 2052 Size: 1275 777 BmrI ACTGGGnnnn_n' Cuts at: 0 1375 1766 2052 Size: 1375 391 286 Fragments arranged by size: 1375 391 286 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 103 2052 Size: 103 1949 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 1109 1127 2052 Size: 146 963 18 925 Fragments arranged by size: 963 925 146 18 Bpu10I CC'TnA_GC Cuts at: 0 1992 2052 Size: 1992 60 BsaI GGTCTCn'nnnn_ Cuts at: 0 1229 1310 1763 1841 2052 Size: 1229 81 453 78 211 Fragments arranged by size: 1229 453 211 81 78 BsaHI Gr'CG_yC Cuts at: 0 1100 1505 2052 Size: 1100 405 547 Fragments arranged by size: 1100 547 405 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1817 2052 Size: 1817 235 BseYI C'CCAG_C Cuts at: 0 226 339 1060 1068 1372 1419 1764 2052 Size: 226 113 721 8 304 47 345 288 Fragments arranged by size: 721 345 304 288 226 113 47 8 BsiEI CG_ry'CG Cuts at: 0 65 1284 2052 Size: 65 1219 768 Fragments arranged by size: 1219 768 65 BsiHKAI G_wGCw'C Cuts at: 0 978 1911 2052 Size: 978 933 141 Fragments arranged by size: 978 933 141 BsmI GAATG_Cn' Cuts at: 0 566 2052 Size: 566 1486 Bsp1286I G_dGCh'C Cuts at: 0 179 198 297 523 554 653 688 725 Size: 179 19 99 226 31 99 35 37 Cuts at: 725 814 978 994 1156 1167 1194 1426 1481 Size: 89 164 16 162 11 27 232 55 Cuts at: 1481 1691 1781 1859 1880 1911 1965 2052 Size: 210 90 78 21 31 54 87 Fragments arranged by size: 232 226 210 179 164 162 99 99 90 89 87 78 55 54 37 35 31 31 27 21 19 16 11 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1321 2052 Size: 1321 731 BsrBI CCG'CTC Cuts at: 0 27 386 1024 2052 Size: 27 359 638 1028 Fragments arranged by size: 1028 638 359 27 BsrFI r'CCGG_y Cuts at: 0 655 1078 2052 Size: 655 423 974 Fragments arranged by size: 974 655 423 BssHII G'CGCG_C Cuts at: 0 12 2052 Size: 12 2040 BstEII G'GTnAC_C Cuts at: 0 631 793 838 1714 2052 Size: 631 162 45 876 338 Fragments arranged by size: 876 631 338 162 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 1380 2052 Size: 1380 672 BstYI r'GATC_y Cuts at: 0 767 1935 1979 2052 Size: 767 1168 44 73 Fragments arranged by size: 1168 767 73 44 Bsu36I CC'TnA_GG Cuts at: 0 1310 1630 2052 Size: 1310 320 422 Fragments arranged by size: 1310 422 320 BtgI C'CryG_G Cuts at: 0 411 825 1746 2052 Size: 411 414 921 306 Fragments arranged by size: 921 414 411 306 EaeI y'GGCC_r Cuts at: 0 2 62 129 878 918 945 1143 1896 Size: 2 60 67 749 40 27 198 753 Cuts at: 1896 2052 Size: 156 Fragments arranged by size: 753 749 198 156 67 60 40 27 2 EagI C'GGCC_G Cuts at: 0 62 2052 Size: 62 1990 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 146 499 518 1109 1127 1862 2052 Size: 146 353 19 591 18 735 190 Fragments arranged by size: 735 591 353 190 146 19 18 EcoO109I rG'GnC_Cy Cuts at: 0 176 194 195 319 424 460 541 550 Size: 176 18 1 124 105 36 81 9 Cuts at: 550 551 577 684 685 722 739 810 990 Size: 1 26 107 1 37 17 71 180 Cuts at: 990 991 1163 1164 1180 1252 1260 1306 1369 Size: 1 172 1 16 72 8 46 63 Cuts at: 1369 1423 1441 1478 1688 1696 1732 1855 1856 Size: 54 18 37 210 8 36 123 1 Cuts at: 1856 1864 1961 2052 Size: 8 97 91 Fragments arranged by size: 210 180 176 172 124 123 107 105 97 91 81 72 71 63 54 46 37 37 36 36 26 18 18 17 16 9 8 8 8 1 1 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1655 2052 Size: 1655 397 FseI GG_CCGG'CC Cuts at: 0 1082 2052 Size: 1082 970 HaeII r_GCGC'y Cuts at: 0 506 781 1727 2052 Size: 506 275 946 325 Fragments arranged by size: 946 506 325 275 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1361 1365 2052 Size: 1361 4 687 Fragments arranged by size: 1361 687 4 MscI TGG'CCA Cuts at: 0 947 1145 2052 Size: 947 198 907 Fragments arranged by size: 947 907 198 MspA1I CmG'CkG Cuts at: 0 413 827 1159 1376 1457 1675 1748 2052 Size: 413 414 332 217 81 218 73 304 Fragments arranged by size: 414 413 332 304 218 217 81 73 NaeI GCC'GGC Cuts at: 0 657 1080 2052 Size: 657 423 972 Fragments arranged by size: 972 657 423 NgoMIV G'CCGG_C Cuts at: 0 655 1078 2052 Size: 655 423 974 Fragments arranged by size: 974 655 423 NotI GC'GGCC_GC Cuts at: 0 62 2052 Size: 62 1990 NspI r_CATG'y Cuts at: 0 906 1122 2052 Size: 906 216 930 Fragments arranged by size: 930 906 216 PasI CC'CwG_GG Cuts at: 0 109 298 547 646 727 1257 1437 1684 Size: 109 189 249 99 81 530 180 247 Cuts at: 1684 1965 2052 Size: 281 87 Fragments arranged by size: 530 281 249 247 189 180 109 99 87 81 PfoI T'CCnGG_A Cuts at: 0 465 978 1185 1290 1929 2052 Size: 465 513 207 105 639 123 Fragments arranged by size: 639 513 465 207 123 105 PpuMI rG'GwC_Cy Cuts at: 0 319 424 460 541 577 739 1369 1441 Size: 319 105 36 81 36 162 630 72 Cuts at: 1441 1696 1732 1864 2052 Size: 255 36 132 188 Fragments arranged by size: 630 319 255 188 162 132 105 81 72 36 36 36 PshAI GACnn'nnGTC Cuts at: 0 737 1286 2052 Size: 737 549 766 Fragments arranged by size: 766 737 549 PspOMI G'GGCC_C Cuts at: 0 175 194 550 649 684 721 810 990 Size: 175 19 356 99 35 37 89 180 Cuts at: 990 1163 1422 1477 1687 1777 1855 1961 2052 Size: 173 259 55 210 90 78 106 91 Fragments arranged by size: 356 259 210 180 175 173 106 99 91 90 89 78 55 37 35 19 PspXI vC'TCGA_Gb Cuts at: 0 1727 2052 Size: 1727 325 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1317 1349 2052 Size: 1317 32 703 Fragments arranged by size: 1317 703 32 PstI C_TGCA'G Cuts at: 0 683 1502 2052 Size: 683 819 550 Fragments arranged by size: 819 683 550 PvuII CAG'CTG Cuts at: 0 1675 2052 Size: 1675 377 SacII CC_GC'GG Cuts at: 0 414 828 1749 2052 Size: 414 414 921 303 Fragments arranged by size: 921 414 414 303 SanDI GG'GwC_CC Cuts at: 0 319 577 739 1369 1441 2052 Size: 319 258 162 630 72 611 Fragments arranged by size: 630 611 319 258 162 72 SbfI CC_TGCA'GG Cuts at: 0 1502 2052 Size: 1502 550 SexAI A'CCwGG_T Cuts at: 0 852 2052 Size: 852 1200 SfcI C'TryA_G Cuts at: 0 679 1498 2052 Size: 679 819 554 Fragments arranged by size: 819 679 554 SfiI GGCCn_nnn'nGGCC Cuts at: 0 101 413 720 1178 1250 1259 2052 Size: 101 312 307 458 72 9 793 Fragments arranged by size: 793 458 312 307 101 72 9 SmaI CCC'GGG Cuts at: 0 120 182 326 336 422 665 691 1476 Size: 120 62 144 10 86 243 26 785 Cuts at: 1476 1511 1650 1776 2052 Size: 35 139 126 276 Fragments arranged by size: 785 276 243 144 139 126 120 86 62 35 26 10 SmlI C'TyrA_G Cuts at: 0 1727 2052 Size: 1727 325 SphI G_CATG'C Cuts at: 0 906 1122 2052 Size: 906 216 930 Fragments arranged by size: 930 906 216 SrfI GCCC'GGGC Cuts at: 0 336 2052 Size: 336 1716 StuI AGG'CCT Cuts at: 0 353 2052 Size: 353 1699 StyI C'CwwG_G Cuts at: 0 287 833 860 1860 1869 2001 2052 Size: 287 546 27 1000 9 132 51 Fragments arranged by size: 1000 546 287 132 51 27 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 497 812 1060 1450 2052 Size: 497 315 248 390 602 Fragments arranged by size: 602 497 390 315 248 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 777 809 1425 1457 2052 Size: 777 32 616 32 595 Fragments arranged by size: 777 616 595 32 32 Tth111I GACn'n_nGTC Cuts at: 0 854 2052 Size: 854 1198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1761 2052 Size: 1761 291 XhoI C'TCGA_G Cuts at: 0 1727 2052 Size: 1727 325 XmaI C'CCGG_G Cuts at: 0 118 180 324 334 420 663 689 1474 Size: 118 62 144 10 86 243 26 785 Cuts at: 1474 1509 1648 1774 2052 Size: 35 139 126 278 Fragments arranged by size: 785 278 243 144 139 126 118 86 62 35 26 10 Enzymes that do cut: AcuI ApaI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstEII BstXI BstYI Bsu36I BtgI EaeI EagI Eco57MI EcoO109I FalI FseI HaeII MmeI MscI MspA1I NaeI NgoMIV NotI NspI PasI PfoI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacII SanDI SbfI SexAI SfcI SfiI SmaI SmlI SphI SrfI StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BbeI BfrBI BglII BmtI BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI NarI NcoI NdeI NheI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI PvuI RsrII SacI SalI SapI ScaI SfoI SgfI SgrAI SnaBI SpeI SspI SwaI TatI XbaI XmnI ZraI