(Circular) (Six-base) MAPSORT of: pFN21AE2439.seq Check: 7392 from: 1 to: 5134 pFN21AE2439 5134 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 2192 Size: 5134 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2944 278 Size: 53 83 186 936 1408 2468 Fragments arranged by size: 2468 1408 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2477 1937 Size: 540 4594 Acc65I G'GTAC_C Cuts at: 2455 2455 Size: 5134 AclI AA'CG_TT Cuts at: 1563 3261 3634 1563 Size: 1698 373 3063 Fragments arranged by size: 3063 1698 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 3197 4581 4849 1632 Size: 713 852 1384 268 1917 Fragments arranged by size: 1917 1384 852 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4937 AflIII A'CryG_T Cuts at: 1203 4033 5010 1203 Size: 2830 977 1327 Fragments arranged by size: 2830 1327 977 AgeI A'CCGG_T Cuts at: 3965 3965 Size: 5134 AhdI GACnn_n'nnGTC Cuts at: 3863 3863 Size: 5134 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5134 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5134 AlwNI CAG_nnn'CTG Cuts at: 2877 4449 2877 Size: 1572 3562 ApaI G_GGCC'C Cuts at: 2136 2194 2136 Size: 58 5076 ApaLI G'TGCA_C Cuts at: 3191 4347 3191 Size: 1156 3978 ApoI r'AATT_y Cuts at: 1493 2443 2669 2821 4930 1493 Size: 950 226 152 2109 1697 Fragments arranged by size: 2109 1697 950 226 152 AseI AT'TA_AT Cuts at: 160 1032 3688 160 Size: 872 2656 1606 Fragments arranged by size: 2656 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5134 AvaI C'yCGr_G Cuts at: 1870 1943 2101 2459 1870 Size: 73 158 358 4545 Fragments arranged by size: 4545 358 158 73 BamHI G'GATC_C Cuts at: 2464 2831 2464 Size: 367 4767 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 2218 2300 2318 2455 Size: 325 217 627 393 38 82 18 137 Cuts at: 2455 3910 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 393 325 217 137 82 38 18 BanII G_rGCy'C Cuts at: 1391 2136 2194 2453 1391 Size: 745 58 259 4072 Fragments arranged by size: 4072 745 259 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3325 3359 1903 Size: 34 50 34 1304 34 3678 Fragments arranged by size: 3678 1304 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3026 4236 3026 Size: 1210 3924 BclI T'GATC_A Cuts at: 1548 1548 Size: 5134 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 3745 136 Size: 107 122 71 631 605 376 1697 1525 Fragments arranged by size: 1697 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 5129 5129 Size: 5134 BlpI GC'TnA_GC Cuts at: 2551 2551 Size: 5134 Bme1580I G_kGCm'C Cuts at: 1790 2136 2183 2194 2223 2297 3195 4351 1790 Size: 346 47 11 29 74 898 1156 2573 Fragments arranged by size: 2573 1156 898 346 74 47 29 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2284 2865 3823 Size: 416 53 83 399 24 867 581 958 Cuts at: 3823 442 Size: 1753 Fragments arranged by size: 1753 958 867 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5134 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2039 2039 Size: 5134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3794 1239 Size: 2555 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3259 4124 4422 3259 Size: 865 298 3971 Fragments arranged by size: 3971 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 3797 882 Size: 617 852 1446 2219 Fragments arranged by size: 2219 1446 852 617 BsaAI yAC'GTr Cuts at: 493 1204 2925 493 Size: 711 1721 2702 Fragments arranged by size: 2702 1721 711 BsaBI GATnn'nnATC Cuts at: 5128 5128 Size: 5134 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2941 3323 3958 275 Size: 53 83 186 936 1408 382 635 1451 Fragments arranged by size: 1451 1408 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2208 3567 3965 4239 4386 4797 2208 Size: 1359 398 274 147 411 2545 Fragments arranged by size: 2545 1359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4909 Fragments arranged by size: 4909 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2018 2027 1186 Size: 829 3 9 4293 Fragments arranged by size: 4293 829 9 3 BseYI C'CCAG_C Cuts at: 2144 2199 4337 4833 2144 Size: 55 2138 496 2445 Fragments arranged by size: 2445 2138 496 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 2233 2365 1163 Size: 573 386 111 132 3932 Fragments arranged by size: 3932 573 386 132 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2067 2584 3345 4373 1540 Size: 275 186 66 517 761 1028 2301 Fragments arranged by size: 2301 1028 761 517 275 186 66 BsiHKAI G_wGCw'C Cuts at: 3195 3280 4351 3195 Size: 85 1071 3978 Fragments arranged by size: 3978 1071 85 BsmI GAATG_Cn' Cuts at: 2645 2738 2645 Size: 93 5041 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2183 2194 2223 2297 2453 3195 Size: 399 346 47 11 29 74 156 742 Cuts at: 3195 3280 4351 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 346 156 85 74 47 29 11 BspEI T'CCGG_A Cuts at: 4797 4797 Size: 5134 BspHI T'CATG_A Cuts at: 3023 4753 3023 Size: 1730 3404 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2192 2489 844 Size: 1348 297 3489 Fragments arranged by size: 3489 1348 297 BsrBI CCG'CTC Cuts at: 1394 1823 3021 1394 Size: 429 1198 3507 Fragments arranged by size: 3507 1198 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3629 3803 65 Size: 1183 2381 174 1396 Fragments arranged by size: 2381 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3778 3965 1700 Size: 110 1968 187 2869 Fragments arranged by size: 2869 1968 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5134 BssHII G'CGCG_C Cuts at: 1925 2333 1925 Size: 408 4726 BssSI C'ACGA_G Cuts at: 3194 4206 3194 Size: 1012 4122 BstAPI GCAn_nnn'nTGC Cuts at: 794 2157 2211 794 Size: 1363 54 3717 Fragments arranged by size: 3717 1363 54 BstBI TT'CG_AA Cuts at: 3938 3938 Size: 5134 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5134 BstYI r'GATC_y Cuts at: 1972 2464 2831 3216 3233 4685 4771 4783 5129 Size: 492 367 385 17 1452 86 12 346 Cuts at: 5129 1972 Size: 1977 Fragments arranged by size: 1977 1452 492 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2061 513 Size: 552 996 3586 Fragments arranged by size: 3586 996 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4395 Fragments arranged by size: 4395 683 56 BtsI GCAGTG_nn' Cuts at: 2650 3444 3464 2650 Size: 794 20 4320 Fragments arranged by size: 4320 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5134 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5099 DraI TTT'AAA Cuts at: 2438 2438 Size: 5134 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4141 809 Size: 507 2825 1802 Fragments arranged by size: 2825 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2581 3470 5099 Size: 54 1425 50 275 18 751 889 1629 Cuts at: 5099 8 Size: 43 Fragments arranged by size: 1629 1425 889 751 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2581 1537 Size: 275 769 4090 Fragments arranged by size: 4090 769 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3064 1353 Size: 1711 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2300 3721 4095 4241 387 Size: 1064 138 711 1421 374 146 1280 Fragments arranged by size: 1421 1280 1064 711 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 3197 3794 4581 4849 1239 Size: 393 713 852 597 787 268 1524 Fragments arranged by size: 1524 852 787 713 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2327 2327 Size: 5134 EcoO109I rG'GnC_Cy Cuts at: 2132 2133 2190 2132 Size: 1 57 5076 Fragments arranged by size: 5076 57 1 EcoRI G'AATT_C Cuts at: 2443 2821 2443 Size: 378 4756 FspI TGC'GCA Cuts at: 3640 3640 Size: 5134 HaeII r_GCGC'y Cuts at: 1678 2054 4281 1678 Size: 376 2227 2531 Fragments arranged by size: 2531 2227 376 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3775 3807 3849 3881 1373 Size: 32 135 32 2203 32 42 32 2626 Fragments arranged by size: 2626 2203 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2478 2724 669 Size: 988 281 540 246 3079 Fragments arranged by size: 3079 988 540 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5134 HpaI GTT'AAC Cuts at: 2724 2724 Size: 5134 KpnI G_GTAC'C Cuts at: 2459 2459 Size: 5134 MfeI C'AATT_G Cuts at: 2733 2733 Size: 5134 MluI A'CGCG_T Cuts at: 5010 5010 Size: 5134 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4248 4432 1305 Size: 2943 184 2007 Fragments arranged by size: 2943 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3312 Fragments arranged by size: 3312 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3092 3451 3610 518 Size: 648 1926 359 159 2042 Fragments arranged by size: 2042 1926 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2550 3227 4375 4620 1606 Size: 457 487 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 487 457 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4582 NdeI CA'TA_TG Cuts at: 387 387 Size: 5134 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5134 NotI GC'GGCC_GC Cuts at: 2581 2581 Size: 5134 NspI r_CATG'y Cuts at: 1132 1229 2492 4037 1132 Size: 97 1263 1545 2229 Fragments arranged by size: 2229 1545 1263 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5134 PciI A'CATG_T Cuts at: 4033 4033 Size: 5134 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5134 PmeI GTTT'AAAC Cuts at: 2438 2438 Size: 5134 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3203 3235 4716 4748 3203 Size: 32 1481 32 3589 Fragments arranged by size: 3589 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5134 PsiI TTA'TAA Cuts at: 2704 2704 Size: 5134 PspOMI G'GGCC_C Cuts at: 2132 2190 2132 Size: 58 5076 PstI C_TGCA'G Cuts at: 830 2486 830 Size: 1656 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5134 SacII CC_GC'GG Cuts at: 2064 2064 Size: 5134 SalI G'TCGA_C Cuts at: 1936 2476 1936 Size: 540 4594 SbfI CC_TGCA'GG Cuts at: 2486 2486 Size: 5134 ScaI AGT'ACT Cuts at: 3382 5094 3382 Size: 1712 3422 SfcI C'TryA_G Cuts at: 826 1045 2482 3617 4298 4489 826 Size: 219 1437 1135 681 191 1471 Fragments arranged by size: 1471 1437 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5134 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5134 SmaI CCC'GGG Cuts at: 1872 2103 2461 1872 Size: 231 358 4545 Fragments arranged by size: 4545 358 231 SmlI C'TyrA_G Cuts at: 820 1017 1943 3238 4139 4401 820 Size: 197 926 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2925 493 Size: 2432 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5134 SphI G_CATG'C Cuts at: 1132 2492 1132 Size: 1360 3774 SspI AAT'ATT Cuts at: 5 52 3058 5 Size: 47 3006 2081 Fragments arranged by size: 3006 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5134 StyI C'CwwG_G Cuts at: 513 1065 2314 2573 513 Size: 552 1249 259 3074 Fragments arranged by size: 3074 1249 552 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3161 3178 3331 3516 3961 325 Size: 1255 1581 17 153 185 445 1498 Fragments arranged by size: 1581 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 3380 5092 Size: 274 80 33 51 1443 282 1121 1712 Cuts at: 5092 96 Size: 138 Fragments arranged by size: 1712 1443 1121 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5094 5126 5094 Size: 32 5102 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5134 XbaI T'CTAG_A Cuts at: 2470 2470 Size: 5134 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5134 XmaI C'CCGG_G Cuts at: 1870 2101 2459 1870 Size: 231 358 4545 Fragments arranged by size: 4545 358 231 XmnI GAAnn'nnTTC Cuts at: 3263 3263 Size: 5134 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2942 276 Size: 53 83 186 936 1408 2468 Fragments arranged by size: 2468 1408 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI