(Circular) (Six-base) MAPSORT of: pFN21AB7688.seq Check: 6002 from: 1 to: 5935 pFN21AB7688 5935 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2787 2787 Size: 5935 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2511 3278 1937 Size: 574 767 4594 Fragments arranged by size: 4594 767 574 Acc65I G'GTAC_C Cuts at: 3256 3256 Size: 5935 AclI AA'CG_TT Cuts at: 1563 4062 4435 1563 Size: 2499 373 3063 Fragments arranged by size: 3063 2499 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 3998 5382 5650 1632 Size: 1292 1074 1384 268 1917 Fragments arranged by size: 1917 1384 1292 1074 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 4834 5811 1203 Size: 3631 977 1327 Fragments arranged by size: 3631 1327 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 2507 4664 2507 Size: 2157 3778 AleI CACnn'nnGTG Cuts at: 1166 2231 1166 Size: 1065 4870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2534 2566 2270 Size: 32 232 32 5639 Fragments arranged by size: 5639 232 32 32 AlwNI CAG_nnn'CTG Cuts at: 3678 5250 3678 Size: 1572 4363 ApaLI G'TGCA_C Cuts at: 3992 5148 3992 Size: 1156 4779 ApoI r'AATT_y Cuts at: 1493 2843 3244 3470 3622 5731 1493 Size: 1350 401 226 152 2109 1697 Fragments arranged by size: 2109 1697 1350 401 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 2075 2129 3260 1870 Size: 73 132 54 1131 4545 Fragments arranged by size: 4545 1131 132 73 54 BamHI G'GATC_C Cuts at: 3265 3632 3265 Size: 367 5568 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3256 4711 618 Size: 325 217 627 1469 1455 1842 Fragments arranged by size: 1842 1469 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3254 1391 Size: 1863 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2044 2816 2961 928 Size: 971 145 772 145 3902 Fragments arranged by size: 3902 971 772 145 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2414 2448 4126 4160 1903 Size: 34 477 34 1678 34 3678 Fragments arranged by size: 3678 1678 477 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 136 Size: 107 122 71 631 605 2874 1525 Fragments arranged by size: 2874 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 3996 5152 1790 Size: 2206 1156 2573 Fragments arranged by size: 2573 2206 1156 BmgBI CAC'GTC Cuts at: 1318 3063 1318 Size: 1745 4190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3666 4624 442 Size: 416 53 83 399 24 2249 958 1753 Fragments arranged by size: 2249 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2951 3014 3139 4595 1239 Size: 1712 63 125 1456 2579 Fragments arranged by size: 2579 1712 1456 125 63 Bpu10I CC'TnA_GC Cuts at: 2601 3018 2601 Size: 417 5518 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2030 4060 4925 5223 2030 Size: 2030 865 298 2742 Fragments arranged by size: 2742 2030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2203 3144 4598 882 Size: 617 704 941 1454 2219 Fragments arranged by size: 2219 1454 941 704 617 BsaAI yAC'GTr Cuts at: 493 1204 3004 3726 493 Size: 711 1800 722 2702 Fragments arranged by size: 2702 1800 722 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3169 4368 4766 5040 5187 5598 3169 Size: 1199 398 274 147 411 3506 Fragments arranged by size: 3506 1199 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2558 2564 2588 3137 Size: 30 165 30 1131 24 6 24 549 Cuts at: 3137 3167 1178 Size: 30 3946 Fragments arranged by size: 3946 1131 549 165 30 30 30 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 2650 3212 1186 Size: 1098 366 562 3909 Fragments arranged by size: 3909 1098 562 366 BseYI C'CCAG_C Cuts at: 2181 2255 5138 5634 2181 Size: 74 2883 496 2482 Fragments arranged by size: 2883 2482 496 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 2735 3126 1163 Size: 573 669 330 391 3972 Fragments arranged by size: 3972 669 573 391 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3996 4081 5152 3996 Size: 85 1071 4779 Fragments arranged by size: 4779 1071 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3254 3996 4081 5152 1391 Size: 399 1464 742 85 1071 2174 Fragments arranged by size: 2174 1464 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2787 3044 3290 844 Size: 1943 257 246 3489 Fragments arranged by size: 3489 1943 257 246 BsrBI CCG'CTC Cuts at: 1394 1823 2128 3822 1394 Size: 429 305 1694 3507 Fragments arranged by size: 3507 1694 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 4430 4604 65 Size: 1183 927 2255 174 1396 Fragments arranged by size: 2255 1396 1183 927 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2212 2724 2748 4579 4766 1700 Size: 110 208 194 512 24 1831 187 2869 Fragments arranged by size: 2869 1831 512 208 194 187 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 2096 3995 5007 2096 Size: 1899 1012 3024 Fragments arranged by size: 3024 1899 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 2143 2922 794 Size: 1221 128 779 3807 Fragments arranged by size: 3807 1221 779 128 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstXI CCAn_nnnn'nTGG Cuts at: 2977 2992 2977 Size: 15 5920 BstYI r'GATC_y Cuts at: 1972 3265 3632 4017 4034 5486 5572 5584 5930 Size: 1293 367 385 17 1452 86 12 346 Cuts at: 5930 1972 Size: 1977 Fragments arranged by size: 1977 1452 1293 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 3451 4245 4265 3451 Size: 794 20 5121 Fragments arranged by size: 5121 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 3239 3239 Size: 5935 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3172 4942 809 Size: 507 1856 1770 1802 Fragments arranged by size: 1856 1802 1770 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2535 2750 Size: 54 1425 50 275 18 270 435 215 Cuts at: 2750 2967 3120 3382 4271 5900 8 Size: 217 153 262 889 1629 43 Fragments arranged by size: 1629 1425 889 435 275 270 262 217 215 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3865 1353 Size: 2512 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 2951 3014 3139 3998 4595 5382 Size: 393 1292 27 63 125 859 597 787 Cuts at: 5382 5650 1239 Size: 268 1524 Fragments arranged by size: 1524 1292 859 787 597 393 268 125 63 27 EcoO109I rG'GnC_Cy Cuts at: 2347 2347 Size: 5935 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 5935 FspI TGC'GCA Cuts at: 4441 4441 Size: 5935 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2493 2525 4576 4608 4650 Size: 32 135 32 921 32 2051 32 42 Cuts at: 4650 4682 1373 Size: 32 2626 Fragments arranged by size: 2626 2051 921 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 2173 2797 3279 3525 669 Size: 988 281 184 51 624 482 246 3079 Fragments arranged by size: 3079 988 624 482 281 246 184 51 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 3260 3260 Size: 5935 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 3010 5049 5233 1305 Size: 1008 697 2039 184 2007 Fragments arranged by size: 2039 2007 1008 697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2537 2969 3122 10 Size: 54 1425 343 705 432 153 2823 Fragments arranged by size: 2823 1425 705 432 343 153 54 MslI CAynn'nnrTG Cuts at: 518 1166 2231 2897 3893 4252 4411 518 Size: 648 1065 666 996 359 159 2042 Fragments arranged by size: 2042 1065 996 666 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3150 3351 4028 5176 5421 1606 Size: 1544 201 677 1148 245 2120 Fragments arranged by size: 2120 1544 1148 677 245 201 NaeI GCC'GGC Cuts at: 2750 2750 Size: 5935 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4444 Fragments arranged by size: 4444 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NgoMIV G'CCGG_C Cuts at: 2748 2748 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 2732 3293 4838 1132 Size: 97 1503 561 1545 2229 Fragments arranged by size: 2229 1545 1503 561 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 4834 4834 Size: 5935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 1108 Size: 1080 4855 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2534 2566 4004 4036 5517 5549 2534 Size: 32 1438 32 1481 32 2920 Fragments arranged by size: 2920 1481 1438 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5935 PsiI TTA'TAA Cuts at: 3505 3505 Size: 5935 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2670 2702 2670 Size: 32 5903 PstI C_TGCA'G Cuts at: 830 2828 3287 830 Size: 1998 459 3478 Fragments arranged by size: 3478 1998 459 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SexAI A'CCwGG_T Cuts at: 2709 2709 Size: 5935 SfcI C'TryA_G Cuts at: 826 1045 2687 2824 3283 4418 5099 5290 826 Size: 219 1642 137 459 1135 681 191 1471 Fragments arranged by size: 1642 1471 1135 681 459 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 2077 3262 1872 Size: 205 1185 4545 Fragments arranged by size: 4545 1185 205 SmlI C'TyrA_G Cuts at: 820 1017 1943 2045 4039 4940 5202 820 Size: 197 926 102 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 2732 3293 1132 Size: 1600 561 3774 Fragments arranged by size: 3774 1600 561 SspI AAT'ATT Cuts at: 5 52 3859 5 Size: 47 3807 2081 Fragments arranged by size: 3807 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 2004 2300 2553 2985 3374 513 Size: 552 939 296 253 432 389 3074 Fragments arranged by size: 3074 939 552 432 389 296 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2776 3962 3979 4132 4317 4762 325 Size: 1255 1196 1186 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1196 1186 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3144 4181 5893 Size: 274 80 33 51 1443 1167 1037 1712 Cuts at: 5893 96 Size: 138 Fragments arranged by size: 1712 1443 1167 1037 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2558 2590 5895 5927 2558 Size: 32 3305 32 2566 Fragments arranged by size: 3305 2566 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 3271 3271 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 2075 3260 1870 Size: 205 1185 4545 Fragments arranged by size: 4545 1185 205 XmnI GAAnn'nnTTC Cuts at: 2682 4064 2682 Size: 1382 4553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI