################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII ClaI DpnII DraI DrdI Eam1105I EarI Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeIII MamI MboI MfeI MflI MluI MroI MseI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcyI 37 GGCGCC G[GA]^CG[CT]C Aor51HI 51 AGCGCT AGC^GCT BalI 296 TGGCCA TGG^CCA BbeI 37 GGCGCC GGCGC^C BbvCI 540 GCTGAGG CCTCAGC(-5/-2) BpmI 562 CTGGAG CTGGAG(16/14) BsaHI 37 GGCGCC G[GA]^CG[CT]C BsaI 350 GGTCTC GGTCTC(1/5) BsaWI 581 TCCGGT [AT]^CCGG[AT] BsgI 508 GTGCAG GTGCAG(16/14) BsmAI 351 GTCTC GTCTC(1/5) BstXI 440 CCATCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 19 CGGACCG CG^G[AT]CCG DraII 636 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 481 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 359 CGGCCG C^GGCCG EclXI 359 CGGCCG C^GGCCG Eco47III 51 AGCGCT AGC^GCT Eco52I 359 CGGCCG C^GGCCG EcoO109I 636 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 518 CCAAGG C^C[AT][AT]GG HgaI 434 GACGC GACGC(5/10) Hin1I 37 GGCGCC G[GA]^CG[CT]C HinfI 532 GAGTC G^A[ACGT]TC HphI 618 GGTGA GGTGA(8/7) KasI 37 GGCGCC G^GCGCC MaeII 82 ACGT A^CGT MboII 552 GAAGA GAAGA(8/7) MluNI 296 TGGCCA TGG^CCA MscI 296 TGGCCA TGG^CCA MslI 441 CATCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 37 GGCGCC GG^CGCC NlaIII 397 CATG CATG^ NotI 358 GCGGCCGC GC^GGCCGC PleI 532 GAGTC GAGTC(4/5) PstI 67 CTGCAG CTGCA^G PvuII 265 CAGCTG CAG^CTG RsrII 19 CGGACCG CG^G[AT]CCG SfcI 67 CTGCAG C^T[GA][CT]AG SfoI 37 GGCGCC GGC^GCC StyI 518 CCAAGG C^C[AT][AT]GG TaqI 125 TCGA T^CGA TthHB8I 125 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwNI 138 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 374 CAGCGGCTG BanI 37 GGCGCC G^G[CT][GA]CC 627 GGCACC BfaI 49 CTAG C^TAG 97 CTAG BsiEI 359 CGGCCG CG[GA][CT]^CG 456 CGACCG BsmFI 12 GGGAC GGGAC(10/14) 647 GGGAC BsrI 561 ACTGG ACTGG(1/-1) 260 CCAGT BssHII 209 GCGCGC G^CGCGC 253 GCGCGC BstAPI 373 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 480 GCACGCCGTGC DdeI 353 CTCAG C^T[ACGT]AG 541 CTGAG MaeI 49 CTAG C^TAG 97 CTAG SacI 100 GAGCTC GAGCT^C 523 GAGCTC TspRI 224 CACTG CA[GC]TG[ACGT][ACGT]^ 238 CAGTG XcmI 275 CCAACCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 518 CCAAGGAGCTCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AccII AciI AluI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DsaI EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI KspI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV SacII Sau96I ScrFI SmaI TseI XmaCI XmaI