(Circular) (Six-base) MAPSORT of: pFN21AB4992.seq Check: 3321 from: 1 to: 5548 pFN21AB4992 5548 bp With 182 enzymes: SgfI * October 20, 2009 10:59 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2489 3358 278 Size: 53 83 186 936 953 869 2468 Fragments arranged by size: 2468 953 936 869 186 83 53 AccI GT'mk_AC Cuts at: 1937 2891 1937 Size: 954 4594 Acc65I G'GTAC_C Cuts at: 2869 2869 Size: 5548 AclI AA'CG_TT Cuts at: 1563 3675 4048 1563 Size: 2112 373 3063 Fragments arranged by size: 3063 2112 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2668 3611 4995 5263 1632 Size: 1036 943 1384 268 1917 Fragments arranged by size: 1917 1384 1036 943 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5351 AflIII A'CryG_T Cuts at: 1203 4447 5424 1203 Size: 3244 977 1327 Fragments arranged by size: 3244 1327 977 AgeI A'CCGG_T Cuts at: 4379 4379 Size: 5548 AhdI GACnn_n'nnGTC Cuts at: 4277 4277 Size: 5548 AleI CACnn'nnGTG Cuts at: 1166 2030 1166 Size: 864 4684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2152 1184 Size: 968 4580 AlwNI CAG_nnn'CTG Cuts at: 3291 4863 3291 Size: 1572 3976 ApaLI G'TGCA_C Cuts at: 2171 3605 4761 2171 Size: 1434 1156 2958 Fragments arranged by size: 2958 1434 1156 ApoI r'AATT_y Cuts at: 1493 2303 2342 2607 2857 3083 3235 5344 1493 Size: 810 39 265 250 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 265 250 226 152 39 AseI AT'TA_AT Cuts at: 160 1032 4102 160 Size: 872 3070 1606 Fragments arranged by size: 3070 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 AvaI C'yCGr_G Cuts at: 1870 1943 2873 1870 Size: 73 930 4545 Fragments arranged by size: 4545 930 73 BamHI G'GATC_C Cuts at: 2878 3245 2878 Size: 367 5181 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2869 4324 618 Size: 325 217 627 1082 1455 1842 Fragments arranged by size: 1842 1455 1082 627 325 217 BanII G_rGCy'C Cuts at: 1391 2709 2867 1391 Size: 1318 158 4072 Fragments arranged by size: 4072 1318 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2838 928 Size: 971 939 3638 Fragments arranged by size: 3638 971 939 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3739 3773 1903 Size: 34 1802 34 3678 Fragments arranged by size: 3678 1802 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3440 4650 3440 Size: 1210 4338 BclI T'GATC_A Cuts at: 1548 2431 1548 Size: 883 4665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4159 136 Size: 107 122 71 631 605 2487 1525 Fragments arranged by size: 2487 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2436 5543 2436 Size: 3107 2441 BlpI GC'TnA_GC Cuts at: 2528 2965 2528 Size: 437 5111 Bme1580I G_kGCm'C Cuts at: 1790 2175 3609 4765 1790 Size: 385 1434 1156 2573 Fragments arranged by size: 2573 1434 1156 385 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5548 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3279 4237 442 Size: 416 53 83 399 24 1862 958 1753 Fragments arranged by size: 1862 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5548 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2681 4208 1239 Size: 1442 1527 2579 Fragments arranged by size: 2579 1527 1442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2197 3673 4538 4836 2197 Size: 1476 865 298 2909 Fragments arranged by size: 2909 1476 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4211 882 Size: 617 2712 2219 Fragments arranged by size: 2712 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3339 493 Size: 711 2135 2702 Fragments arranged by size: 2702 2135 711 BsaBI GATnn'nnATC Cuts at: 5542 5542 Size: 5548 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2486 3355 3737 4372 Size: 53 83 186 936 953 869 382 635 Cuts at: 4372 275 Size: 1451 Fragments arranged by size: 1451 953 936 869 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2556 3981 4379 4653 4800 5211 2556 Size: 1425 398 274 147 411 2893 Fragments arranged by size: 2893 1425 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5323 Fragments arranged by size: 5323 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5548 BseYI C'CCAG_C Cuts at: 4751 5247 4751 Size: 496 5052 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 1163 Size: 573 336 4639 Fragments arranged by size: 4639 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2998 3759 4787 1540 Size: 275 186 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2709 3609 3694 4765 2175 Size: 534 900 85 1071 2958 Fragments arranged by size: 2958 1071 900 534 85 BsmI GAATG_Cn' Cuts at: 2550 3059 3152 2550 Size: 509 93 4946 Fragments arranged by size: 4946 509 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2709 2867 3609 3694 4765 1391 Size: 399 385 534 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 534 399 385 158 85 BspEI T'CCGG_A Cuts at: 2556 5211 2556 Size: 2655 2893 BspHI T'CATG_A Cuts at: 3437 5167 3437 Size: 1730 3818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2903 844 Size: 2059 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3435 1394 Size: 429 1612 3507 Fragments arranged by size: 3507 1612 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2539 4043 4217 65 Size: 1183 1291 1504 174 1396 Fragments arranged by size: 1504 1396 1291 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2523 4192 4379 1700 Size: 110 713 1669 187 2869 Fragments arranged by size: 2869 1669 713 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5548 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5548 BssSI C'ACGA_G Cuts at: 3608 4620 3608 Size: 1012 4536 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5548 BstBI TT'CG_AA Cuts at: 4352 4352 Size: 5548 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5548 BstYI r'GATC_y Cuts at: 1972 2436 2878 3245 3630 3647 5099 5185 5197 Size: 464 442 367 385 17 1452 86 12 Cuts at: 5197 5543 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 464 442 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2799 2799 Size: 5548 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4057 Fragments arranged by size: 4057 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4809 Fragments arranged by size: 4809 683 56 BtsI GCAGTG_nn' Cuts at: 3064 3858 3878 3064 Size: 794 20 4734 Fragments arranged by size: 4734 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5548 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5513 DraI TTT'AAA Cuts at: 2852 2852 Size: 5548 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4555 809 Size: 507 3239 1802 Fragments arranged by size: 3239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2521 2587 Size: 54 1425 50 275 18 177 514 66 Cuts at: 2587 2995 3884 5513 8 Size: 408 889 1629 43 Fragments arranged by size: 1629 1425 889 514 408 275 177 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2995 1537 Size: 275 1183 4090 Fragments arranged by size: 4090 1183 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 2811 3478 1353 Size: 809 649 667 3423 Fragments arranged by size: 3423 809 667 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4509 4655 387 Size: 1064 138 2546 374 146 1280 Fragments arranged by size: 2546 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2707 2707 Size: 5548 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2668 2681 3611 4208 4995 5263 1239 Size: 393 1036 13 930 597 787 268 1524 Fragments arranged by size: 1524 1036 930 787 597 393 268 13 EcoNI CCTnn'n_nnAGG Cuts at: 2424 2424 Size: 5548 EcoRI G'AATT_C Cuts at: 2857 3235 2857 Size: 378 5170 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 2362 Size: 5548 FspI TGC'GCA Cuts at: 4054 4054 Size: 5548 HaeII r_GCGC'y Cuts at: 1678 2414 4695 1678 Size: 736 2281 2531 Fragments arranged by size: 2531 2281 736 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4189 4221 4263 4295 1373 Size: 32 135 32 2617 32 42 32 2626 Fragments arranged by size: 2626 2617 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 2266 2892 3138 669 Size: 988 281 139 189 626 246 3079 Fragments arranged by size: 3079 988 626 281 246 189 139 HindIII A'AGCT_T Cuts at: 748 748 Size: 5548 HpaI GTT'AAC Cuts at: 3138 3138 Size: 5548 KpnI G_GTAC'C Cuts at: 2873 2873 Size: 5548 MfeI C'AATT_G Cuts at: 3147 3147 Size: 5548 MluI A'CGCG_T Cuts at: 5424 5424 Size: 5548 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4662 4846 1305 Size: 3357 184 2007 Fragments arranged by size: 3357 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3726 Fragments arranged by size: 3726 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2150 2727 3506 3865 4024 518 Size: 648 864 120 577 779 359 159 2042 Fragments arranged by size: 2042 864 779 648 577 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2629 2964 3641 4789 5034 1606 Size: 1023 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 677 335 245 NaeI GCC'GGC Cuts at: 2525 2525 Size: 5548 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4057 Fragments arranged by size: 4057 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5548 NgoMIV G'CCGG_C Cuts at: 2523 2523 Size: 5548 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5548 NotI GC'GGCC_GC Cuts at: 2995 2995 Size: 5548 NspI r_CATG'y Cuts at: 1132 1229 2155 2512 2906 4451 1132 Size: 97 926 357 394 1545 2229 Fragments arranged by size: 2229 1545 926 394 357 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5548 PciI A'CATG_T Cuts at: 4447 4447 Size: 5548 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 2629 1108 Size: 1038 483 4027 Fragments arranged by size: 4027 1038 483 PmeI GTTT'AAAC Cuts at: 2852 2852 Size: 5548 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 5130 5162 3617 Size: 32 1481 32 4003 Fragments arranged by size: 4003 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5548 PsiI TTA'TAA Cuts at: 3118 3118 Size: 5548 PstI C_TGCA'G Cuts at: 830 2900 830 Size: 2070 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5548 PvuII CAG'CTG Cuts at: 2629 2629 Size: 5548 SacI G_AGCT'C Cuts at: 2709 2709 Size: 5548 SalI G'TCGA_C Cuts at: 1936 2890 1936 Size: 954 4594 SapI GCTCTTCn'nnn_ Cuts at: 2162 2811 2162 Size: 649 4899 SbfI CC_TGCA'GG Cuts at: 2900 2900 Size: 5548 ScaI AGT'ACT Cuts at: 3796 5508 3796 Size: 1712 3836 SfcI C'TryA_G Cuts at: 826 1045 2280 2896 4031 4712 4903 826 Size: 219 1235 616 1135 681 191 1471 Fragments arranged by size: 1471 1235 1135 681 616 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5548 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5548 SmaI CCC'GGG Cuts at: 1872 2875 1872 Size: 1003 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2176 3652 4553 4815 820 Size: 197 926 233 1476 901 262 1553 Fragments arranged by size: 1553 1476 926 901 262 233 197 SnaBI TAC'GTA Cuts at: 493 3339 493 Size: 2846 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5548 SphI G_CATG'C Cuts at: 1132 2906 1132 Size: 1774 3774 SspI AAT'ATT Cuts at: 5 52 3472 5 Size: 47 3420 2081 Fragments arranged by size: 3420 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5548 StyI C'CwwG_G Cuts at: 513 1065 2004 2987 513 Size: 552 939 983 3074 Fragments arranged by size: 3074 983 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2249 3575 3592 3745 3930 4375 325 Size: 1255 669 1326 17 153 185 445 1498 Fragments arranged by size: 1498 1326 1255 669 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 3794 5506 Size: 274 80 33 51 1443 42 1775 1712 Cuts at: 5506 96 Size: 138 Fragments arranged by size: 1775 1712 1443 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2458 2490 5508 5540 2458 Size: 32 3018 32 2466 Fragments arranged by size: 3018 2466 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5548 XbaI T'CTAG_A Cuts at: 2884 2884 Size: 5548 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5548 XmaI C'CCGG_G Cuts at: 1870 2873 1870 Size: 1003 4545 XmnI GAAnn'nnTTC Cuts at: 3677 3677 Size: 5548 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2487 3356 276 Size: 53 83 186 936 953 869 2468 Fragments arranged by size: 2468 953 936 869 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI