################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII AccII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KspI MamI MluI MluNI MscI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1184 GGTACC G^GTACC AccI 853 GTAGAC GT^[AC][GT]AC AccIII 1113 TCCGGA T^CCGGA AcsI 593 AAATTC [GA]^AATT[CT] AcyI 1297 GACGCC G[GA]^CG[CT]C Alw44I 1022 GTGCAC G^TGCAC AlwNI 1330 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1022 GTGCAC G^TGCAC ApoI 593 AAATTC [GA]^AATT[CT] Asp718I 1184 GGTACC G^GTACC BamHI 1110 GGATCC G^GATCC BanI 1184 GGTACC G^G[CT][GA]CC BbsI 1363 GAAGAC GAAGAC(2/6) BbvCI 196 GCTGAGG CCTCAGC(-5/-2) BciVI 439 GGATAC GTATCC(6/5) BcnI 216 CCGGG CC^[GC]GG BglII 1282 AGATCT A^GATCT BpuAI 1363 GAAGAC GAAGAC(2/6) BsaAI 433 CACGTA [CT]AC^GT[GA] BsaHI 1297 GACGCC G[GA]^CG[CT]C BsaI 682 GGTCTC GGTCTC(1/5) BseAI 1113 TCCGGA T^CCGGA BsgI 707 GTGCAG GTGCAG(16/14) BsmI 1239 GAATGC GAATGC(1/-1) Bsp1407I 794 TGTACA T^GTACA BspEI 1113 TCCGGA T^CCGGA BspHI 1137 TCATGA T^CATGA BspMI 1161 ACCTGC ACCTGC(4/8) BsrGI 794 TGTACA T^GTACA BssSI 817 CTCGTG CACGAG(-5/-1) BstXI 1100 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 238 GGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1301 CCATGG C^C[GA][CT]GG EaeI 841 CGGCCA [CT]^GGCC[GA] EcoO109I 238 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1003 GATATC GAT^ATC Hin1I 1297 GACGCC G[GA]^CG[CT]C HincII 549 GTTGAC GT[CT]^[GA]AC HindII 549 GTTGAC GT[CT]^[GA]AC KpnI 1184 GGTACC GGTAC^C MaeII 434 ACGT A^CGT MfeI 1123 CAATTG C^AATTG MroI 1113 TCCGGA T^CCGGA MunI 1123 CAATTG C^AATTG MwoI 1300 GCCATGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 216 CCGGG CC^[GC]GG NcoI 1301 CCATGG C^CATGG PflMI 339 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 238 GGGACCT [GA]G^G[AT]CC[CT] PvuII 798 CAGCTG CAG^CTG RcaI 1137 TCATGA T^CATGA SapI 566 GAAGAGC GCTCTTC(1/4) SfcI 518 CTACAG C^T[GA][CT]AG SspBI 794 TGTACA T^GTACA Van91I 339 CCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 880 TCTAGA T^CTAGA XcmI 335 CCATCCACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AciI 228 GCGG CCGC(-3/-1) 840 GCGG AflIII 857 ACATGT A^C[GA][CT]GT 992 ACATGT AlwI 1111 GATCC GGATC(4/5) 1110 GGATC Asp700I 607 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1129 GAAACCCTTC AspHI 430 GAGCAC G[AT]GC[AT]^C 1022 GTGCAC BfaI 881 CTAG C^TAG 1294 CTAG BmyI 430 GAGCAC G[AGT]GC[ACT]^C 1022 GTGCAC BpmI 162 CTCCAG CTGGAG(16/14) 1286 CTCCAG BsaWI 1113 TCCGGA [AT]^CCGG[AT] 1181 TCCGGT BsiHKAI 430 GAGCAC G[AT]GC[AT]^C 1022 GTGCAC BsmFI 238 GGGAC GGGAC(10/14) 751 GTCCC Bsp1286I 430 GAGCAC G[AGT]GC[ACT]^C 1022 GTGCAC BspLU11I 857 ACATGT A^CATGT 992 ACATGT BsrDI 399 CATTGC GCAATG(2/0) 687 CATTGC BstNI 235 CCAGG CC^[AT]GG 525 CCAGG BstYI 1110 GGATCC [GA]^GATC[CT] 1282 AGATCT EarI 140 GAAGAG CTCTTC(1/4) 566 GAAGAG EcoRII 235 CCAGG ^CC[AT]GG 525 CCAGG HgaI 813 GACGC GACGC(5/10) 1297 GACGC HphI 348 GGTGA GGTGA(8/7) 967 GGTGA Ksp632I 140 GAAGAG CTCTTC(1/4) 566 GAAGAG MaeI 881 CTAG C^TAG 1294 CTAG MflI 1110 GGATCC [GA]^GATC[CT] 1282 AGATCT MspA1I 226 CAGCGG C[AC]G^C[GT]G 798 CAGCTG MvaI 235 CCAGG CC^[AT]GG 525 CCAGG NdeI 478 CATATG CA^TATG 1067 CATATG PleI 331 GAGTC GAGTC(4/5) 877 GAGTC SfaNI 1072 GATGC GCATC(5/9) 863 GCATC TaqI 90 TCGA T^CGA 956 TCGA TthHB8I 90 TCGA T^CGA 956 TCGA XhoII 1110 GGATCC [GA]^GATC[CT] 1282 AGATCT XmnI 607 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1129 GAAACCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AfaI AluI AvaII BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I Cac8I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MseI MslI MspI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI