(Circular) (Six-base) MAPSORT of: pFN21AB4011.seq Check: 1058 from: 1 to: 6358 pFN21AB4011 6358 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2145 2145 Size: 6358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4168 278 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3701 1937 Size: 1764 4594 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 4485 4858 1563 Size: 2922 373 3063 Fragments arranged by size: 3063 2922 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2327 2432 2654 2748 2828 3215 4421 Size: 635 60 105 222 94 80 387 1206 Cuts at: 4421 5805 6073 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1206 635 387 268 222 105 94 80 60 AfeI AGC'GCT Cuts at: 3110 3110 Size: 6358 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6161 AflIII A'CryG_T Cuts at: 1203 2611 5257 6234 1203 Size: 1408 2646 977 1327 Fragments arranged by size: 2646 1408 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 3240 1166 Size: 2074 4284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 1184 Size: 1206 5152 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2989 2957 Size: 32 6326 AlwNI CAG_nnn'CTG Cuts at: 3094 3253 4101 5673 3094 Size: 159 848 1572 3779 Fragments arranged by size: 3779 1572 848 159 ApaI G_GGCC'C Cuts at: 2040 2040 Size: 6358 ApaLI G'TGCA_C Cuts at: 2158 3539 4415 5571 2158 Size: 1381 876 1156 2945 Fragments arranged by size: 2945 1381 1156 876 ApoI r'AATT_y Cuts at: 1493 3667 3893 4045 6154 1493 Size: 2174 226 152 2109 1697 Fragments arranged by size: 2174 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4912 160 Size: 872 3880 1606 Fragments arranged by size: 3880 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 2417 3683 1870 Size: 73 474 1266 4545 Fragments arranged by size: 4545 1266 474 73 BamHI G'GATC_C Cuts at: 2133 2845 3688 4055 2133 Size: 712 843 367 4436 Fragments arranged by size: 4436 843 712 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2780 3234 3340 3679 5134 Size: 325 217 627 993 454 106 339 1455 Cuts at: 5134 618 Size: 1842 Fragments arranged by size: 1842 1455 993 627 454 339 325 217 106 BanII G_rGCy'C Cuts at: 1391 2040 2127 2319 2528 3677 1391 Size: 649 87 192 209 1149 4072 Fragments arranged by size: 4072 1149 649 209 192 87 BbeI G_GCGC'C Cuts at: 3344 3344 Size: 6358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5387 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3001 3035 4549 4583 1903 Size: 34 50 34 980 34 1514 34 3678 Fragments arranged by size: 3678 1514 980 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3080 4969 136 Size: 107 122 71 631 605 1408 1889 1525 Fragments arranged by size: 1889 1525 1408 631 605 122 107 71 BglII A'GATC_T Cuts at: 6353 6353 Size: 6358 BlpI GC'TnA_GC Cuts at: 2394 3775 2394 Size: 1381 4977 Bme1580I G_kGCm'C Cuts at: 1790 2040 2162 2554 2774 3303 3543 4419 5575 Size: 250 122 392 220 529 240 876 1156 Cuts at: 5575 1790 Size: 2573 Fragments arranged by size: 2573 1156 876 529 392 250 240 220 122 BmgBI CAC'GTC Cuts at: 1318 2542 1318 Size: 1224 5134 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2564 4089 5047 Size: 416 53 83 399 24 1147 1525 958 Cuts at: 5047 442 Size: 1753 Fragments arranged by size: 1753 1525 1147 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6358 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 3533 5018 1239 Size: 869 1425 1485 2579 Fragments arranged by size: 2579 1485 1425 869 Bpu10I CC'TnA_GC Cuts at: 2059 3161 2059 Size: 1102 5256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4483 5348 5646 4483 Size: 865 298 5195 Fragments arranged by size: 5195 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2009 5021 882 Size: 617 510 3012 2219 Fragments arranged by size: 3012 2219 617 510 BsaAI yAC'GTr Cuts at: 493 1204 2614 4149 493 Size: 711 1410 1535 2702 Fragments arranged by size: 2702 1535 1410 711 BsaBI GATnn'nnATC Cuts at: 6352 6352 Size: 6358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2576 3341 4165 4547 Size: 53 83 186 936 1043 765 824 382 Cuts at: 4547 5182 275 Size: 635 1451 Fragments arranged by size: 1451 1043 936 824 765 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4791 5189 5463 5610 6021 4791 Size: 398 274 147 411 5128 Fragments arranged by size: 5128 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 2114 2144 1178 Size: 30 165 30 678 30 3 30 5392 Fragments arranged by size: 5392 678 165 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 3005 3215 1186 Size: 1705 114 210 4329 Fragments arranged by size: 4329 1705 210 114 BseYI C'CCAG_C Cuts at: 2170 3253 3490 5561 6057 2170 Size: 1083 237 2071 496 2471 Fragments arranged by size: 2471 2071 1083 496 237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3443 1163 Size: 573 1707 4078 Fragments arranged by size: 4078 1707 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2162 2319 2528 3282 3543 4419 4504 5575 2162 Size: 157 209 754 261 876 85 1071 2945 Fragments arranged by size: 2945 1071 876 754 261 209 157 85 BsmI GAATG_Cn' Cuts at: 3869 3962 3869 Size: 93 6265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2127 2162 2319 2528 2554 2774 Size: 399 250 87 35 157 209 26 220 Cuts at: 2774 3282 3303 3543 3677 4419 4504 5575 1391 Size: 508 21 240 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 250 240 220 209 157 134 87 85 35 26 21 BspEI T'CCGG_A Cuts at: 6021 6021 Size: 6358 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 3713 844 Size: 1301 1568 3489 Fragments arranged by size: 3489 1568 1301 BsrBI CCG'CTC Cuts at: 1394 1823 2058 3646 4245 1394 Size: 429 235 1588 599 3507 Fragments arranged by size: 3507 1588 599 429 235 BsrDI GCAATG_nn' Cuts at: 65 1248 2793 4853 5027 65 Size: 1183 1545 2060 174 1396 Fragments arranged by size: 2060 1545 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2175 5002 5189 1700 Size: 110 365 2827 187 2869 Fragments arranged by size: 2869 2827 365 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 3542 4418 5430 3542 Size: 876 1012 4470 Fragments arranged by size: 4470 1012 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 3094 794 Size: 2300 4058 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 3604 1184 Size: 2420 3938 BstXI CCAn_nnnn'nTGG Cuts at: 2947 2947 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2133 2721 2845 3312 3471 3549 3688 4055 Size: 161 588 124 467 159 78 139 367 Cuts at: 4055 4440 4457 5909 5995 6007 6353 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 588 467 385 367 346 161 159 139 124 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2940 3345 513 Size: 552 1875 405 3526 Fragments arranged by size: 3526 1875 552 405 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 2205 2487 2738 3055 3874 4668 4688 2205 Size: 282 251 317 819 794 20 3875 Fragments arranged by size: 3875 819 794 317 282 251 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 3662 3662 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 3072 3633 Size: 54 1425 50 275 18 492 750 561 Cuts at: 3633 3805 4694 6323 8 Size: 172 889 1629 43 Fragments arranged by size: 1629 1425 889 750 561 492 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4288 1353 Size: 2935 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3464 4945 5319 5465 387 Size: 1064 138 1875 1481 374 146 1280 Fragments arranged by size: 1875 1481 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2526 2317 Size: 209 6149 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2267 2327 2432 2654 2748 2828 Size: 393 476 159 60 105 222 94 80 Cuts at: 2828 3215 3533 4421 5018 5805 6073 1239 Size: 387 318 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 476 393 387 318 268 222 159 105 94 80 60 EcoNI CCTnn'n_nnAGG Cuts at: 2090 2090 Size: 6358 EcoO109I rG'GnC_Cy Cuts at: 2036 2097 3205 2036 Size: 61 1108 5189 Fragments arranged by size: 5189 1108 61 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 FseI GG_CCGG'CC Cuts at: 2179 2179 Size: 6358 FspI TGC'GCA Cuts at: 3087 4864 3087 Size: 1777 4581 HaeII r_GCGC'y Cuts at: 1678 3112 3344 5505 1678 Size: 1434 232 2161 2531 Fragments arranged by size: 2531 2161 1434 232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2426 2458 2476 Size: 32 135 32 509 32 313 32 18 Cuts at: 2476 2508 2817 2849 4999 5031 5073 5105 1373 Size: 32 309 32 2150 32 42 32 2626 Fragments arranged by size: 2626 2150 509 313 309 135 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3702 3948 669 Size: 988 281 1764 246 3079 Fragments arranged by size: 3079 1764 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6358 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KasI G'GCGC_C Cuts at: 3340 3340 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 3957 3957 Size: 6358 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5472 5656 1305 Size: 4167 184 2007 Fragments arranged by size: 4167 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2324 10 Size: 54 1425 343 492 4044 Fragments arranged by size: 4044 1425 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2504 2507 2756 2840 3240 4316 Size: 648 1101 237 3 249 84 400 1076 Cuts at: 4316 4675 4834 518 Size: 359 159 2042 Fragments arranged by size: 2042 1101 1076 648 400 359 249 237 159 84 3 MspA1I CmG'CkG Cuts at: 1606 2170 2714 3271 3583 3774 4451 5599 5844 Size: 564 544 557 312 191 677 1148 245 Cuts at: 5844 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 564 557 544 312 245 191 NaeI GCC'GGC Cuts at: 2177 2177 Size: 6358 NarI GG'CG_CC Cuts at: 3341 3341 Size: 6358 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5806 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NgoMIV G'CCGG_C Cuts at: 2175 2175 Size: 6358 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6358 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 2755 3016 3397 3716 5261 1132 Size: 97 1526 261 381 319 1545 2229 Fragments arranged by size: 2229 1545 1526 381 319 261 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6358 PfoI T'CCnGG_A Cuts at: 2444 2821 3318 3510 2444 Size: 377 497 192 5292 Fragments arranged by size: 5292 497 377 192 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PmlI CAC'GTG Cuts at: 2614 2614 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2981 3013 3383 3415 4427 4459 5940 5972 2981 Size: 32 370 32 1012 32 1481 32 3367 Fragments arranged by size: 3367 1481 1012 370 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 3205 2097 Size: 1108 5250 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PspOMI G'GGCC_C Cuts at: 2036 2036 Size: 6358 PstI C_TGCA'G Cuts at: 830 2730 3099 3183 3710 830 Size: 1900 369 84 527 3478 Fragments arranged by size: 3478 1900 527 369 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 PvuII CAG'CTG Cuts at: 2170 2170 Size: 6358 SacI G_AGCT'C Cuts at: 2319 2528 2319 Size: 209 6149 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SanDI GG'GwC_CC Cuts at: 2097 2097 Size: 6358 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SfcI C'TryA_G Cuts at: 826 1045 2726 3095 3179 3706 4841 5522 5713 Size: 219 1681 369 84 527 1135 681 191 Cuts at: 5713 826 Size: 1471 Fragments arranged by size: 1681 1471 1135 681 527 369 219 191 84 SfoI GGC'GCC Cuts at: 3342 3342 Size: 6358 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 2419 3685 1872 Size: 547 1266 4545 Fragments arranged by size: 4545 1266 547 SmlI C'TyrA_G Cuts at: 820 1017 1943 4462 5363 5625 820 Size: 197 926 2519 901 262 1553 Fragments arranged by size: 2519 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3016 3716 1132 Size: 1884 700 3774 Fragments arranged by size: 3774 1884 700 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6358 StyI C'CwwG_G Cuts at: 513 1065 2118 3797 513 Size: 552 1053 1679 3074 Fragments arranged by size: 3074 1679 1053 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4385 4402 4555 4740 5185 325 Size: 1255 2805 17 153 185 445 1498 Fragments arranged by size: 2805 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4604 6316 96 Size: 274 80 33 51 1443 2627 1712 138 Fragments arranged by size: 2627 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 6318 6350 2114 Size: 32 4172 32 2122 Fragments arranged by size: 4172 2122 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2689 2689 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 2417 3683 1870 Size: 547 1266 4545 Fragments arranged by size: 4545 1266 547 XmnI GAAnn'nnTTC Cuts at: 2297 3329 4487 2297 Size: 1032 1158 4168 Fragments arranged by size: 4168 1158 1032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4166 276 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI